A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae
The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of t...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Oxford University Press (OUP)
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8369 https://academic.oup.com/nargab/article/2/1/lqz022/5680725 https://doi.org/10.1093/nargab/lqz022 |
| _version_ | 1855484185584599040 |
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| author | Ferron, François Debat, Humberto Julio Shannon, Ashleigh Decroly, Etienne Canard, Bruno |
| author_browse | Canard, Bruno Debat, Humberto Julio Decroly, Etienne Ferron, François Shannon, Ashleigh |
| author_facet | Ferron, François Debat, Humberto Julio Shannon, Ashleigh Decroly, Etienne Canard, Bruno |
| author_sort | Ferron, François |
| collection | INTA Digital |
| description | The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine
and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales
order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these
families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome
locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase
with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase. |
| format | Artículo |
| id | INTA8369 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press (OUP) |
| publisherStr | Oxford University Press (OUP) |
| record_format | dspace |
| spelling | INTA83692020-12-02T17:35:39Z A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae Ferron, François Debat, Humberto Julio Shannon, Ashleigh Decroly, Etienne Canard, Bruno Coronaviridae Nidovirales Viruses Genetic Markers RNA ARN Marcadores Genéticos Virus The order Nidovirales is a diverse group of (+) RNA viruses, classified together based on their common genome organisation and conserved replicative enzymes, despite drastic differences in size and complexity. One such difference pertains to the mechanisms and enzymes responsible for generation of the proposed viral 5 RNA cap. Within the Coronaviridae family, two separate methytransferases (MTase), nsp14 and nsp16, perform the RNA-cap N7-guanine and 2 -OH methylation respectively for generation of the proposed m7GpppNm type I cap structure. For the majority of other families within the Nidovirales order, the presence, structure and key enzymes involved in 5 capping are far less clear. These viruses either lack completely an RNA MTase signature sequence, or lack an N7-guanine methyltransferase signature sequence, obscuring our understanding about how RNA-caps are N7-methylated for these families. Here, we report the discovery of a putative Rossmann fold RNA methyltransferase in 10 Tobaniviridae members in Orf1a, an unusual genome locus for this gene. Multiple sequence alignments and structural analyses lead us to propose this novel gene as a typical RNA-cap N7-guanine MTase with substrate specificity and active-site organization similar to the canonical eukaryotic RNA-cap N7-guanine MTase. Instituto de Patología Vegetal Fil: Ferron, François. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Shannon, Ashleigh. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia Fil: Decroly, Etienne. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia Fil: Canard, Bruno. CNRS and Aix-Marseille Université. Architecture et Fonction des Macromolécules Biologiques; Francia. European Virus Bioinformatics Center; Alemania 2020-12-02T17:17:47Z 2020-12-02T17:17:47Z 2019-12-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8369 https://academic.oup.com/nargab/article/2/1/lqz022/5680725 2631-9268 (online) https://doi.org/10.1093/nargab/lqz022 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford University Press (OUP) NAR Genomics and Bioinformatics 2 (1) : lqz022 (March 2020) |
| spellingShingle | Coronaviridae Nidovirales Viruses Genetic Markers RNA ARN Marcadores Genéticos Virus Ferron, François Debat, Humberto Julio Shannon, Ashleigh Decroly, Etienne Canard, Bruno A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title | A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title_full | A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title_fullStr | A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title_full_unstemmed | A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title_short | A N7-guanine RNA cap methyltransferase signature-sequence as a genetic marker of large genome, non-mammalian Tobaniviridae |
| title_sort | n7 guanine rna cap methyltransferase signature sequence as a genetic marker of large genome non mammalian tobaniviridae |
| topic | Coronaviridae Nidovirales Viruses Genetic Markers RNA ARN Marcadores Genéticos Virus |
| url | http://hdl.handle.net/20.500.12123/8369 https://academic.oup.com/nargab/article/2/1/lqz022/5680725 https://doi.org/10.1093/nargab/lqz022 |
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