Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops
Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of t...
| Autores principales: | , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8366 https://link.springer.com/article/10.1007/s42161-020-00686-0 https://doi.org/10.1007/s42161-020-00686-0 |
| _version_ | 1855036022892527616 |
|---|---|
| author | De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis |
| author_browse | Bejerman, Nicolas Esteban De Breuil, Soledad Dottori, Carolina Andrea Giolitti, Fabian Lenardon, Sergio Luis Nome Docampo, Claudia |
| author_facet | De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis |
| author_sort | De Breuil, Soledad |
| collection | INTA Digital |
| description | Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction
enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease
incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8366 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA83662020-12-02T14:36:40Z Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní Argentina is one of the main peanut (Arachis hypogaea L.) exporting countries with the major peanut-growing area located in the province of Córdoba. During the 2015–2016 growing season, severe outbreaks of an orthotospovirus-like disease occurred in a number of commercial peanut fields. The aim of this work was to determine the prevalence of orthotospoviruses and their incidence in peanut. In DAS-ELISA, all samples collected from symptomatic plants reacted with antisera to groundnut ringspot virus/tomato chlorotic spot virus (GRSV/TCSV) and tomato spotted wilt virus (TSWV). RT-PCR was performed with a single pair of primers for conserved regions of the GRSV, TCSV and TSWV nucleoprotein (N) genes, and digestion with restriction enzymes, allowed the identification of the pathogen asGRSV. The results were confirmed by sequencing of the N gene. Diseased peanut plants were observed in 30 out of 81 surveyed fields located in the north-central region of the peanut-growing area. In that region, the disease incidence was evaluated in four commercial fields, recorded values from 3% to 47.25% with a mean disease incidence of 18.69%. Our study reported for the first time a significant outbreak of GRSV in peanut in Argentina and provide information on the occurrence and distribution of some Orthotospovirus species. Instituto de Patología Vegetal Fil: De Breuil, Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Dottori, Carolina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal (IPAVE); Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina 2020-12-02T14:20:28Z 2020-12-02T14:20:28Z 2020-10-13 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8366 https://link.springer.com/article/10.1007/s42161-020-00686-0 1125-4653 2239-7264 (online) https://doi.org/10.1007/s42161-020-00686-0 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of Plant Pathology (October 2020) |
| spellingShingle | Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní De Breuil, Soledad Dottori, Carolina Andrea Bejerman, Nicolas Esteban Nome Docampo, Claudia Giolitti, Fabian Lenardon, Sergio Luis Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title | Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title_full | Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title_fullStr | Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title_full_unstemmed | Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title_short | Orthotospovirus disease epidemic: molecular characterization and incidence in peanut crops |
| title_sort | orthotospovirus disease epidemic molecular characterization and incidence in peanut crops |
| topic | Arachis Hypogaea Argentina Virus de las Plantas Plant Viruses Outbreak Orthotospovirus Maní |
| url | http://hdl.handle.net/20.500.12123/8366 https://link.springer.com/article/10.1007/s42161-020-00686-0 https://doi.org/10.1007/s42161-020-00686-0 |
| work_keys_str_mv | AT debreuilsoledad orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops AT dottoricarolinaandrea orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops AT bejermannicolasesteban orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops AT nomedocampoclaudia orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops AT giolittifabian orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops AT lenardonsergioluis orthotospovirusdiseaseepidemicmolecularcharacterizationandincidenceinpeanutcrops |