High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)

Alfalfa (Medicago sativa L.), also known as lucerne, is a major forage crop worldwide. In the United States, it has recently become the third most valuable field crop, with an estimated value of over $9.3 billion. Alfalfa is naturally infected by many different pathogens, including viruses, obligate...

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Main Authors: Bejerman, Nicolas Esteban, Roumagnac, Philippe, Nemchinov, Lev G.
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Frontiers Media 2020
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2020.553109/full
http://hdl.handle.net/20.500.12123/8365
https://doi.org/10.3389/fmicb.2020.553109
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author Bejerman, Nicolas Esteban
Roumagnac, Philippe
Nemchinov, Lev G.
author_browse Bejerman, Nicolas Esteban
Nemchinov, Lev G.
Roumagnac, Philippe
author_facet Bejerman, Nicolas Esteban
Roumagnac, Philippe
Nemchinov, Lev G.
author_sort Bejerman, Nicolas Esteban
collection INTA Digital
description Alfalfa (Medicago sativa L.), also known as lucerne, is a major forage crop worldwide. In the United States, it has recently become the third most valuable field crop, with an estimated value of over $9.3 billion. Alfalfa is naturally infected by many different pathogens, including viruses, obligate parasites that reproduce only inside living host cells. Traditionally, viral infections of alfalfa have been considered by breeders, growers, producers and researchers to be diseases of limited importance, although they are widespread in all major cultivation areas. However, over the past few years, due to the rapid development of high-throughput sequencing (HTS), viral metagenomics, bioinformatics tools for interpreting massive amounts of HTS data and the increasing accessibility of public data repositories for transcriptomic discoveries, several emerging viruses of alfalfa with the potential to cause serious yield losses have been described. They include alfalfa leaf curl virus (family Geminiviridae), alfalfa dwarf virus (family Rhabdoviridae), alfalfa enamovirus 1 (family Luteoviridae), alfalfa virus S (family Alphaflexiviridae) and others. These discoveries have called into question the assumed low economic impact of viral diseases in alfalfa and further suggested their possible contribution to the severity of complex infections involving multiple pathogens. In this review, we will focus on viruses of alfalfa recently described in different laboratories on the basis of the above research methodologies.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA83652020-12-02T14:17:26Z High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.) Bejerman, Nicolas Esteban Roumagnac, Philippe Nemchinov, Lev G. Medicago Sativa Plantas Forrajeras Plant Viruses Feed Crops Virus de las Plantas Alfalfa Cultivo Forrajero Virome High Throughput Sequencing Emerging Viruses Lucerne Alfalfa (Medicago sativa L.), also known as lucerne, is a major forage crop worldwide. In the United States, it has recently become the third most valuable field crop, with an estimated value of over $9.3 billion. Alfalfa is naturally infected by many different pathogens, including viruses, obligate parasites that reproduce only inside living host cells. Traditionally, viral infections of alfalfa have been considered by breeders, growers, producers and researchers to be diseases of limited importance, although they are widespread in all major cultivation areas. However, over the past few years, due to the rapid development of high-throughput sequencing (HTS), viral metagenomics, bioinformatics tools for interpreting massive amounts of HTS data and the increasing accessibility of public data repositories for transcriptomic discoveries, several emerging viruses of alfalfa with the potential to cause serious yield losses have been described. They include alfalfa leaf curl virus (family Geminiviridae), alfalfa dwarf virus (family Rhabdoviridae), alfalfa enamovirus 1 (family Luteoviridae), alfalfa virus S (family Alphaflexiviridae) and others. These discoveries have called into question the assumed low economic impact of viral diseases in alfalfa and further suggested their possible contribution to the severity of complex infections involving multiple pathogens. In this review, we will focus on viruses of alfalfa recently described in different laboratories on the basis of the above research methodologies. Instituto de Patología Vegetal Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Roumagnac, Philippe. CIRAD, BGPI. Montpellier; France. Université Montpellier. Institut Agro. BGPI, INRAE, CIRAD. Montpellier; Francia Fil: Nemchinov, Lev G. Plant Sciences Institute. Molecular Plant Pathology Laboratory; Estados Unidos 2020-12-02T14:00:32Z 2020-12-02T14:00:32Z 2020-09-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.frontiersin.org/articles/10.3389/fmicb.2020.553109/full http://hdl.handle.net/20.500.12123/8365 1664-302X https://doi.org/10.3389/fmicb.2020.553109 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Microbiology 11 : 553109. (2020)
spellingShingle Medicago Sativa
Plantas Forrajeras
Plant Viruses
Feed Crops
Virus de las Plantas
Alfalfa
Cultivo Forrajero
Virome
High Throughput Sequencing
Emerging Viruses
Lucerne
Bejerman, Nicolas Esteban
Roumagnac, Philippe
Nemchinov, Lev G.
High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title_full High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title_fullStr High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title_full_unstemmed High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title_short High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Medicago sativa L.)
title_sort high throughput sequencing for deciphering the virome of alfalfa medicago sativa l
topic Medicago Sativa
Plantas Forrajeras
Plant Viruses
Feed Crops
Virus de las Plantas
Alfalfa
Cultivo Forrajero
Virome
High Throughput Sequencing
Emerging Viruses
Lucerne
url https://www.frontiersin.org/articles/10.3389/fmicb.2020.553109/full
http://hdl.handle.net/20.500.12123/8365
https://doi.org/10.3389/fmicb.2020.553109
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