Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species
This paper presents the first acute toxicity data of the natural insecticide spinosad in amphibians. The sensitivity of two neotropical sympatric anuran species, Boana pulchella and Rhinella arenarum, to spinosad-based formulation Tracer™ was evaluated. Lethal effects are reported in tadpoles of B....
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8343 https://link.springer.com/article/10.1007/s11356-020-09808-8 https://doi.org/10.1007/s11356-020-09808-8 |
| _version_ | 1855484179628687360 |
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| author | Bahl, María Florencia Brodeur, Celine Marie Salgado Costa, Carolina D'andrea, María Florencia Sansiñena, Jesica Alejandra Marino, Damian Jose Gabriel Natale, Guillermo Sebastián |
| author_browse | Bahl, María Florencia Brodeur, Celine Marie D'andrea, María Florencia Marino, Damian Jose Gabriel Natale, Guillermo Sebastián Salgado Costa, Carolina Sansiñena, Jesica Alejandra |
| author_facet | Bahl, María Florencia Brodeur, Celine Marie Salgado Costa, Carolina D'andrea, María Florencia Sansiñena, Jesica Alejandra Marino, Damian Jose Gabriel Natale, Guillermo Sebastián |
| author_sort | Bahl, María Florencia |
| collection | INTA Digital |
| description | This paper presents the first acute toxicity data of the natural insecticide spinosad in amphibians. The sensitivity of two neotropical sympatric anuran species, Boana pulchella and Rhinella arenarum, to spinosad-based formulation Tracer™ was evaluated. Lethal effects are reported in tadpoles of B. pulchella stage 25 between 2.81 and 35.44 mg spinosad/L, while for the same concentration range no lethal effects were detected in tadpoles of R. arenarum of the same stage. In addition, Tracer™ produced sublethal effects at the individual level on the swimming activity, morphology (growth and presence of abnormalities), and development of B. pulchella from 2.81 to 5.78 mg spinosad/L, while in R. arenarum effects were only detected in the swimming activity and growth from 2.78 and 6.22 mg/L, respectively. At the biochemical level, Tracer™ produced inhibition of different enzymatic activities, among them, catalase activity at 2.81 mg spinosad/L, glutathione S- transferase activity from 2.81 to 2.98 mg spinosad/L, and acetylcholinesterase activity at 2.81 mg spinosad/L. These findings allow us to conclude that B. pulchella is more sensitive than R. arenarum to spinosad-based formulation Tracer™. The effects demonstrated here are not consistent with those expected since spinosad is supposed to be an environmental healthy alternative. This paper provides useful and necessary information to implement regulations on the use of new compounds entering the market and its associated risks. |
| format | Artículo |
| id | INTA8343 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA83432024-02-29T16:28:02Z Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species Bahl, María Florencia Brodeur, Celine Marie Salgado Costa, Carolina D'andrea, María Florencia Sansiñena, Jesica Alejandra Marino, Damian Jose Gabriel Natale, Guillermo Sebastián Anura Espinosad Insecticidas Mortalidad Spinosad Insecticides Mortality This paper presents the first acute toxicity data of the natural insecticide spinosad in amphibians. The sensitivity of two neotropical sympatric anuran species, Boana pulchella and Rhinella arenarum, to spinosad-based formulation Tracer™ was evaluated. Lethal effects are reported in tadpoles of B. pulchella stage 25 between 2.81 and 35.44 mg spinosad/L, while for the same concentration range no lethal effects were detected in tadpoles of R. arenarum of the same stage. In addition, Tracer™ produced sublethal effects at the individual level on the swimming activity, morphology (growth and presence of abnormalities), and development of B. pulchella from 2.81 to 5.78 mg spinosad/L, while in R. arenarum effects were only detected in the swimming activity and growth from 2.78 and 6.22 mg/L, respectively. At the biochemical level, Tracer™ produced inhibition of different enzymatic activities, among them, catalase activity at 2.81 mg spinosad/L, glutathione S- transferase activity from 2.81 to 2.98 mg spinosad/L, and acetylcholinesterase activity at 2.81 mg spinosad/L. These findings allow us to conclude that B. pulchella is more sensitive than R. arenarum to spinosad-based formulation Tracer™. The effects demonstrated here are not consistent with those expected since spinosad is supposed to be an environmental healthy alternative. This paper provides useful and necessary information to implement regulations on the use of new compounds entering the market and its associated risks. Instituto de Recursos Biológicos Fil: Bahl, María Florencia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Brodeur, Celine Marie Julie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Bilológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Salgado Costa, Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: D'Andrea, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Bilológicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Sansiñena, Jesica Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Marino, Damián José. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Natale, Guillermo S. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Química. Centro de Investigaciones del Medio Ambiente; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2020-11-30T12:20:44Z 2020-11-30T12:20:44Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8343 https://link.springer.com/article/10.1007/s11356-020-09808-8 0944-1344 1614-7499 https://doi.org/10.1007/s11356-020-09808-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Environmental Science and Pollution Research (2020) |
| spellingShingle | Anura Espinosad Insecticidas Mortalidad Spinosad Insecticides Mortality Bahl, María Florencia Brodeur, Celine Marie Salgado Costa, Carolina D'andrea, María Florencia Sansiñena, Jesica Alejandra Marino, Damian Jose Gabriel Natale, Guillermo Sebastián Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title | Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title_full | Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title_fullStr | Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title_full_unstemmed | Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title_short | Lethal and sublethal effects of the natural and healthy spinosad-based formulation Tracer™ on tadpoles of two neotropical species |
| title_sort | lethal and sublethal effects of the natural and healthy spinosad based formulation tracer™ on tadpoles of two neotropical species |
| topic | Anura Espinosad Insecticidas Mortalidad Spinosad Insecticides Mortality |
| url | http://hdl.handle.net/20.500.12123/8343 https://link.springer.com/article/10.1007/s11356-020-09808-8 https://doi.org/10.1007/s11356-020-09808-8 |
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