Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth
Chloris gayana is a warm-season grass, often cultivated in areas where soil salinity is a major constraint for forage production. Five cultivars (2 unselected populations and 3 synthetic varieties) were evaluated through agronomic traits as well as Inter Simple Sequence Repeats (ISSRs) and Sequence-...
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| Format: | Artículo |
| Language: | Inglés |
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CIAT
2020
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| Online Access: | https://www.tropicalgrasslands.info/index.php/tgft/article/view/449 http://hdl.handle.net/20.500.12123/8342 https://doi.org/10.17138/tgft(7)14-24 |
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| author | Ribotta, Andrea Noemi Lopez Colomba, Eliana Bollati, Graciela P. Striker, Gustavo Gabriel Carloni, Edgardo José Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina |
| author_browse | Bollati, Graciela P. Carloni, Edgardo José Griffa, Sabrina Mariana Grunberg, Karina Lopez Colomba, Eliana Quiroga, Mariana Paola Ribotta, Andrea Noemi Striker, Gustavo Gabriel Tommasino, Exequiel Arturo |
| author_facet | Ribotta, Andrea Noemi Lopez Colomba, Eliana Bollati, Graciela P. Striker, Gustavo Gabriel Carloni, Edgardo José Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina |
| author_sort | Ribotta, Andrea Noemi |
| collection | INTA Digital |
| description | Chloris gayana is a warm-season grass, often cultivated in areas where soil salinity is a major constraint for forage production. Five cultivars (2 unselected populations and 3 synthetic varieties) were evaluated through agronomic traits as well as Inter Simple Sequence Repeats (ISSRs) and Sequence-related Amplified Polymorphism (SRAP) molecular markers. The consensus between both agronomic and molecular data sets was high (>99%) suggesting that both systems provided similar estimates of genetic relationships. The analysis revealed that synthetic varieties, Finecut, Topcut and Santana, were the most genetically different cultivars, whereas the unselected populations, Pioneer and Katambora, were closely related. Responses to salinity stress during germination and early vegetative growth stages were evaluated in only the synthetic varieties. The results showed that Finecut and Santana were able to germinate in the same proportion as controls even at concentrations of 200 mM NaCl. Under hydroponic conditions, Santana attained approximately 20% higher total dry weight than the other 2 varieties and the longest roots. Finecut presented the highest root dry weight. These results suggested that Santana and Finecut showed high salinity tolerance at germination and early vegetative growth stages, both crucial phases when seeking a successful pasture establishment, particularly in saline environments. Further studies in the field are needed to determine if these hydroponic results are reproduced under field conditions. |
| format | Artículo |
| id | INTA8342 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | CIAT |
| publisherStr | CIAT |
| record_format | dspace |
| spelling | INTA83422020-11-30T11:37:29Z Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth Ribotta, Andrea Noemi Lopez Colomba, Eliana Bollati, Graciela P. Striker, Gustavo Gabriel Carloni, Edgardo José Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina Chloris gayana Crecimiento Marcadores Genéticos Tolerancia a la Sal Variedades Growth Genetic Markers Salt Tolerance Varieties Marcadores Moleculares Chloris gayana is a warm-season grass, often cultivated in areas where soil salinity is a major constraint for forage production. Five cultivars (2 unselected populations and 3 synthetic varieties) were evaluated through agronomic traits as well as Inter Simple Sequence Repeats (ISSRs) and Sequence-related Amplified Polymorphism (SRAP) molecular markers. The consensus between both agronomic and molecular data sets was high (>99%) suggesting that both systems provided similar estimates of genetic relationships. The analysis revealed that synthetic varieties, Finecut, Topcut and Santana, were the most genetically different cultivars, whereas the unselected populations, Pioneer and Katambora, were closely related. Responses to salinity stress during germination and early vegetative growth stages were evaluated in only the synthetic varieties. The results showed that Finecut and Santana were able to germinate in the same proportion as controls even at concentrations of 200 mM NaCl. Under hydroponic conditions, Santana attained approximately 20% higher total dry weight than the other 2 varieties and the longest roots. Finecut presented the highest root dry weight. These results suggested that Santana and Finecut showed high salinity tolerance at germination and early vegetative growth stages, both crucial phases when seeking a successful pasture establishment, particularly in saline environments. Further studies in the field are needed to determine if these hydroponic results are reproduced under field conditions. Chloris gayana es una gramínea subtropical, frecuentemente cultivada en áreas donde la salinidad del suelo es una limitación importante para la producción forrajera. En Córdoba, Argentina, fueron evaluadas por sus características agronómicas y moleculares (ISSR y SRAP) cinco cultivares (dos poblaciones no-selectas y tres variedades sintéticas) de esta especie. El consenso entre ambos tipos de datos fue alto (>99%) sugiriendo que las caracterizaciones proporcionaron estimaciones similares de las relaciones genéticas. Los resultados mostraron que las variedades sintéticas, Finecut, Topcut y Santana, fueron las más genéticamente diferentes mientras que las poblaciones no-selectas, Pioneer y Katambora, estuvieron estrechamente relacionadas. En las tres variedades sintéticas se evaluó también la respuesta a salinidad en germinación y plántula. Los resultados mostraron que Finecut y Santana germinaron en la misma proporción que los controles, incluso a concentraciones de 200 mM NaCl. En condiciones de hidroponía, Santana alcanzó 20% más de peso seco total y raíces más largas que las otras dos variedades. Finecut presentó el mayor peso seco de raíz. Estos resultados sugieren que Santana y Finecut mostraron alta tolerancia a salinidad en germinación y plántula, etapas cruciales cuando se piensa en el establecimiento exitoso de una pastura, particularmente en ambientes salinos. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Bollati, Graciela P. Universidad Católica de Córdoba. Facultad de Ciencias Agropecuarias; Argentina Fil: Striker, Gustavo Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Fisiológicas y Ecológicas vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas vinculadas a la Agricultura; Argentina Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina 2020-11-30T11:30:31Z 2020-11-30T11:30:31Z 2019-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.tropicalgrasslands.info/index.php/tgft/article/view/449 http://hdl.handle.net/20.500.12123/8342 2346-3775 https://doi.org/10.17138/tgft(7)14-24 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf CIAT Tropical Grasslands-Forrajes Tropicales 7 (1) : 14–24 (2019) |
| spellingShingle | Chloris gayana Crecimiento Marcadores Genéticos Tolerancia a la Sal Variedades Growth Genetic Markers Salt Tolerance Varieties Marcadores Moleculares Ribotta, Andrea Noemi Lopez Colomba, Eliana Bollati, Graciela P. Striker, Gustavo Gabriel Carloni, Edgardo José Griffa, Sabrina Mariana Quiroga, Mariana Paola Tommasino, Exequiel Arturo Grunberg, Karina Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title | Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title_full | Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title_fullStr | Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title_full_unstemmed | Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title_short | Agronomic and molecular characterization of Chloris gayana cultivars and salinity response during germination and early vegetative growth |
| title_sort | agronomic and molecular characterization of chloris gayana cultivars and salinity response during germination and early vegetative growth |
| topic | Chloris gayana Crecimiento Marcadores Genéticos Tolerancia a la Sal Variedades Growth Genetic Markers Salt Tolerance Varieties Marcadores Moleculares |
| url | https://www.tropicalgrasslands.info/index.php/tgft/article/view/449 http://hdl.handle.net/20.500.12123/8342 https://doi.org/10.17138/tgft(7)14-24 |
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