Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples
Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 poly...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Mary Ann Liebert Inc.
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8317 https://www.liebertpub.com/doi/10.1089/fpd.2011.0963 http://doi.org/10.1089/fpd.2011.0963 |
| _version_ | 1855036014810103808 |
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| author | Zumarraga, Martin Jose Soutullo, Adriana Garcia, Maria Ines Marini, M. Rocío Abdala, Alejandro Ariel Tarabla, Hector Dante Echaide, Susana T. de Lopez, Marcela Zervini, Elsa Canal, Ana María Cataldi, Angel Adrian |
| author_browse | Abdala, Alejandro Ariel Canal, Ana María Cataldi, Angel Adrian Echaide, Susana T. de Garcia, Maria Ines Lopez, Marcela Marini, M. Rocío Soutullo, Adriana Tarabla, Hector Dante Zervini, Elsa Zumarraga, Martin Jose |
| author_facet | Zumarraga, Martin Jose Soutullo, Adriana Garcia, Maria Ines Marini, M. Rocío Abdala, Alejandro Ariel Tarabla, Hector Dante Echaide, Susana T. de Lopez, Marcela Zervini, Elsa Canal, Ana María Cataldi, Angel Adrian |
| author_sort | Zumarraga, Martin Jose |
| collection | INTA Digital |
| description | Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8317 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Mary Ann Liebert Inc. |
| publisherStr | Mary Ann Liebert Inc. |
| record_format | dspace |
| spelling | INTA83172020-11-24T16:55:37Z Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples Zumarraga, Martin Jose Soutullo, Adriana Garcia, Maria Ines Marini, M. Rocío Abdala, Alejandro Ariel Tarabla, Hector Dante Echaide, Susana T. de Lopez, Marcela Zervini, Elsa Canal, Ana María Cataldi, Angel Adrian Mycobacterium bovis Rebaño de Leche PCR Leche Tuberculosis Argentina Dairy Herds Milk Bovine tuberculosis (bTB) is a chronic and zoonotic disease due to Mycobacterium bovis. The tuberculosis eradication campaign carried out in Argentina has considerably improved the health situation of the herds. Here we evaluated a strategy to detect M. bovis–infected herds by Touch-Down IS6110 polymerase chain reaction (PCR) in bulk tank raw milk from dairy farms. We evaluated 177 samples from herds with the official tuberculosis free certificate (TFC) and 80 from herds without the certificate, non-tuberculosis-free certificate (NTFC), from 10 departments of Santa Fe province, Argentina. To avoid the effect of Taq polymerase inhibitors, a dilution of DNA template was performed. Positive PCR results were obtained in 102 (40%) of the samples, whereas negative ones were obtained in 155 (60%) of the samples. Importantly, 44% of NTFC and 38% of TFC samples were positive. All samples were subjected to culture in Löwenstein Jensen and Stonebrink media with no positive isolation. The negative predictive value (NPV) of PCR in the TFC group was 95%, while the positive predictive value (PPV) of PCR in the NTFC group was 51%. Based on these results, this work proposes a method that should be applied regularly to detect M. bovis--infected dairy herds, complementary to the official test of tuberculin, or purifed protein derivative (PPD), to control dairy herds, especially those free of tuberculosis. Instituto de Biotecnología Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Soutullo, Adriana. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina Fil: Garcia, Maria Ines. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Cátedra de Inmunología Básica; Argentina Fil: Marini, M. Rocío. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Echaide, Susana T. de. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Lopez, Marcela. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina Fil: Zervini, Elsa. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni; Argentina Fil: Canal, Ana María. Ministerio de la Producción. Dirección General de Sanidad Animal. Laboratorio de Diagnóstico e Investigaciones Agropecuarias; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Cátedra de Patología Básica; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-11-24T16:44:41Z 2020-11-24T16:44:41Z 2012-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8317 https://www.liebertpub.com/doi/10.1089/fpd.2011.0963 1556-7125 http://doi.org/10.1089/fpd.2011.0963 eng info:eu-repo/semantics/restrictedAccess application/pdf Mary Ann Liebert Inc. Foodborne Pathogens and Disease 9 (2) : 132-137 (Febrero 2012) |
| spellingShingle | Mycobacterium bovis Rebaño de Leche PCR Leche Tuberculosis Argentina Dairy Herds Milk Zumarraga, Martin Jose Soutullo, Adriana Garcia, Maria Ines Marini, M. Rocío Abdala, Alejandro Ariel Tarabla, Hector Dante Echaide, Susana T. de Lopez, Marcela Zervini, Elsa Canal, Ana María Cataldi, Angel Adrian Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title | Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title_full | Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title_fullStr | Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title_full_unstemmed | Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title_short | Detection of Mycobacterium bovis–infected dairy herds using PCR in bulk tank milk samples |
| title_sort | detection of mycobacterium bovis infected dairy herds using pcr in bulk tank milk samples |
| topic | Mycobacterium bovis Rebaño de Leche PCR Leche Tuberculosis Argentina Dairy Herds Milk |
| url | http://hdl.handle.net/20.500.12123/8317 https://www.liebertpub.com/doi/10.1089/fpd.2011.0963 http://doi.org/10.1089/fpd.2011.0963 |
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