Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection
The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This stud...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Sage Publications
2020
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/8231 https://journals.sagepub.com/doi/10.1177/104063870501700303 https://doi.org/10.1177/104063870501700303 |
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| author | Zumarraga, Martin Jose Meikle, Virginia Bernardelli, Amelia Abdala, Alejandro Ariel Tarabla, Hector Dante Romano, Maria Isabel Cataldi, Angel Adrian |
| author_browse | Abdala, Alejandro Ariel Bernardelli, Amelia Cataldi, Angel Adrian Meikle, Virginia Romano, Maria Isabel Tarabla, Hector Dante Zumarraga, Martin Jose |
| author_facet | Zumarraga, Martin Jose Meikle, Virginia Bernardelli, Amelia Abdala, Alejandro Ariel Tarabla, Hector Dante Romano, Maria Isabel Cataldi, Angel Adrian |
| author_sort | Zumarraga, Martin Jose |
| collection | INTA Digital |
| description | The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification. |
| format | Artículo |
| id | INTA8231 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Sage Publications |
| publisherStr | Sage Publications |
| record_format | dspace |
| spelling | INTA82312020-11-10T15:00:55Z Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection Zumarraga, Martin Jose Meikle, Virginia Bernardelli, Amelia Abdala, Alejandro Ariel Tarabla, Hector Dante Romano, Maria Isabel Cataldi, Angel Adrian Mycobacterium bovis PCR Técnicas de Diagnosis Diagnostic Techniques The confirmatory diagnosis of Mycobacterium bovis (M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification. Instituto de Biotecnología Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Bernardelli, Amelia. Servicio Nacional de Sanidad y Calidad Agroalimentaria. Dirección de Laboratorios y Control Técnico; Argentina Fil: Abdala, Alejandro Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Tarabla, Hector Dante. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-11-10T14:55:13Z 2020-11-10T14:55:13Z 2005-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8231 https://journals.sagepub.com/doi/10.1177/104063870501700303 1943-4936 https://doi.org/10.1177/104063870501700303 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Sage Publications Journal of Veterinary Diagnostic Investigation 17 (3) : 232-238 (Mayo 2005) |
| spellingShingle | Mycobacterium bovis PCR Técnicas de Diagnosis Diagnostic Techniques Zumarraga, Martin Jose Meikle, Virginia Bernardelli, Amelia Abdala, Alejandro Ariel Tarabla, Hector Dante Romano, Maria Isabel Cataldi, Angel Adrian Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title | Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title_full | Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title_fullStr | Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title_full_unstemmed | Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title_short | Use of touch-down polymerase chain reaction to enhance the sensitivity of Mycobacterium bovis detection |
| title_sort | use of touch down polymerase chain reaction to enhance the sensitivity of mycobacterium bovis detection |
| topic | Mycobacterium bovis PCR Técnicas de Diagnosis Diagnostic Techniques |
| url | http://hdl.handle.net/20.500.12123/8231 https://journals.sagepub.com/doi/10.1177/104063870501700303 https://doi.org/10.1177/104063870501700303 |
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