Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts
There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8191 https://www.sciencedirect.com/science/article/abs/pii/S1472979214203908 https://doi.org/10.1016/j.tube.2014.08.003 |
| _version_ | 1855484150330425344 |
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| author | Morcillo, Nora Imperiale, Belen Rocio Di Giulio, Angela Beatríz Zumarraga, Martin Jose Takif, Howard Cataldi, Angel Adrian |
| author_browse | Cataldi, Angel Adrian Di Giulio, Angela Beatríz Imperiale, Belen Rocio Morcillo, Nora Takif, Howard Zumarraga, Martin Jose |
| author_facet | Morcillo, Nora Imperiale, Belen Rocio Di Giulio, Angela Beatríz Zumarraga, Martin Jose Takif, Howard Cataldi, Angel Adrian |
| author_sort | Morcillo, Nora |
| collection | INTA Digital |
| description | There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts. |
| format | Artículo |
| id | INTA8191 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA81912020-11-04T17:17:54Z Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts Morcillo, Nora Imperiale, Belen Rocio Di Giulio, Angela Beatríz Zumarraga, Martin Jose Takif, Howard Cataldi, Angel Adrian Drug Resistance Vigour Households Resistencia a Medicamentos Tuberculosis Vigor Hogares Mycobacterium tuberculosis Family Transmission Transmisión Familiar There has been an on-going debate on whether the development of drug resistance in Mycobacterium tuberculosis reduces its relative fitness and its ability to cause disease. The aim of this study was to explore this relationship. For this purpose, we evaluated the in vitro growth of clinical isolates and the transmission of the strains within the patients' households. Clinical and epidemiological data from patients in households, drug-susceptibility and genetic patterns of the isolates were collected. BACTEC MGIT 960™ system with the Epicenter™ software was used to perform fitness experiments and calculate the relative fitness (RF) comparing with the H73Rv reference strain. From 39 households, 124 patients and 388 contacts were included. Concerning transmission, 20 Multi drug-resistant (MDR) and 16 drug sensitive (DS) index cases generated 23 and 28 secondary cases, respectively. An average RF drop of 16.7% was found for MDR strains, but only mutations in rpoB codons 531 were associated with reduced fitness. When the strains were transmitted, their RF tended to decrease, and strains with low RF were less frequently transmitted. Within the limitations of this study, the results showed that the decrease in RF was associated to a limited transmission among the households' contacts. Instituto de Biotecnología Fil: Morcillo, Nora. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina Fil: Imperiale, Belen Rocio. Hospital Dr. Antonio A. Cetrángolo. Laboratorio de Referencia del Programa de Control de la Tuberculosis de la provincia de Buenos Aires; Argentina Fil: Di Giulio, Angela Beatríz. Hospital Dr. Petrona V. de Cordero. Laboratorio de Micobacterias; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Takif, Howard. Instituto Venezolano de Investigaciones Científicas; Venezuela Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-11-04T17:10:05Z 2020-11-04T17:10:05Z 2014-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8191 https://www.sciencedirect.com/science/article/abs/pii/S1472979214203908 1472-9792 https://doi.org/10.1016/j.tube.2014.08.003 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 94 (6) : 672-677 (Diciembre 2014) |
| spellingShingle | Drug Resistance Vigour Households Resistencia a Medicamentos Tuberculosis Vigor Hogares Mycobacterium tuberculosis Family Transmission Transmisión Familiar Morcillo, Nora Imperiale, Belen Rocio Di Giulio, Angela Beatríz Zumarraga, Martin Jose Takif, Howard Cataldi, Angel Adrian Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title | Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title_full | Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title_fullStr | Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title_full_unstemmed | Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title_short | Fitness of drug resistant Mycobacterium tuberculosis and the impact on the transmission among household contacts |
| title_sort | fitness of drug resistant mycobacterium tuberculosis and the impact on the transmission among household contacts |
| topic | Drug Resistance Vigour Households Resistencia a Medicamentos Tuberculosis Vigor Hogares Mycobacterium tuberculosis Family Transmission Transmisión Familiar |
| url | http://hdl.handle.net/20.500.12123/8191 https://www.sciencedirect.com/science/article/abs/pii/S1472979214203908 https://doi.org/10.1016/j.tube.2014.08.003 |
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