Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8172 https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127 https://doi.org/10.1016/j.tube.2013.02.004 |
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| author | Blanco, Federico Carlos Bianco, María Veronica Garbaccio, Sergio Gabriel Meikle, Virginia Gravisaco, María José Montenegro, Valeria Noely Alfonseca, Edgar Singh, Mahavir G. Barandiaran, Soledad Canal, Ana María Vagnoni, Lucas Emilio Buddle, Bryce Malcom Bigi, Fabiana Cataldi, Angel Adrian |
| author_browse | Alfonseca, Edgar Barandiaran, Soledad Bianco, María Veronica Bigi, Fabiana Blanco, Federico Carlos Buddle, Bryce Malcom Canal, Ana María Cataldi, Angel Adrian Garbaccio, Sergio Gabriel Gravisaco, María José Meikle, Virginia Montenegro, Valeria Noely Singh, Mahavir G. Vagnoni, Lucas Emilio |
| author_facet | Blanco, Federico Carlos Bianco, María Veronica Garbaccio, Sergio Gabriel Meikle, Virginia Gravisaco, María José Montenegro, Valeria Noely Alfonseca, Edgar Singh, Mahavir G. Barandiaran, Soledad Canal, Ana María Vagnoni, Lucas Emilio Buddle, Bryce Malcom Bigi, Fabiana Cataldi, Angel Adrian |
| author_sort | Blanco, Federico Carlos |
| collection | INTA Digital |
| description | A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle. |
| format | Artículo |
| id | INTA8172 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA81722020-11-02T16:08:50Z Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis Blanco, Federico Carlos Bianco, María Veronica Garbaccio, Sergio Gabriel Meikle, Virginia Gravisaco, María José Montenegro, Valeria Noely Alfonseca, Edgar Singh, Mahavir G. Barandiaran, Soledad Canal, Ana María Vagnoni, Lucas Emilio Buddle, Bryce Malcom Bigi, Fabiana Cataldi, Angel Adrian Tuberculosis Infección por Mycobacterium bovis Vacuna Viva Ganado Bovino Organismos Patógenos Virulentos Mycobacterium bovis Infections Live Vaccines Cattle Virulent Pathogens A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle. Instituto de Biotecnología Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; México Fil: Singh, Mahavir G. Lionnex; Alemania Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva Zelanda Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-11-02T15:59:25Z 2020-11-02T15:59:25Z 2013-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8172 https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127 1472-9792 https://doi.org/10.1016/j.tube.2013.02.004 eng info:eu-repograntAgreement/INTA/AERG-232001/AR./Desarrollos biotecnológicos para el control de enfermedades infecciosas en animales de interés económico info:eu-repograntAgreement/INTA/AEBIO-243512/AR./Búsqueda y caracterización de biomarcadores para el control de enfermedades de interés pecuario mediante el empleo de técnicas post genómicas. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 93 (3) : 363-372 (May 2013) |
| spellingShingle | Tuberculosis Infección por Mycobacterium bovis Vacuna Viva Ganado Bovino Organismos Patógenos Virulentos Mycobacterium bovis Infections Live Vaccines Cattle Virulent Pathogens Blanco, Federico Carlos Bianco, María Veronica Garbaccio, Sergio Gabriel Meikle, Virginia Gravisaco, María José Montenegro, Valeria Noely Alfonseca, Edgar Singh, Mahavir G. Barandiaran, Soledad Canal, Ana María Vagnoni, Lucas Emilio Buddle, Bryce Malcom Bigi, Fabiana Cataldi, Angel Adrian Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title_full | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title_fullStr | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title_full_unstemmed | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title_short | Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis |
| title_sort | mycobacterium bovis dmce2 double deletion mutant protects cattle against challenge with virulent m bovis |
| topic | Tuberculosis Infección por Mycobacterium bovis Vacuna Viva Ganado Bovino Organismos Patógenos Virulentos Mycobacterium bovis Infections Live Vaccines Cattle Virulent Pathogens |
| url | http://hdl.handle.net/20.500.12123/8172 https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127 https://doi.org/10.1016/j.tube.2013.02.004 |
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