High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata)
In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for...
| Main Authors: | , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8145 https://link.springer.com/article/10.1007%2Fs11627-020-10098-5 https://doi.org/10.1007/s11627-020-10098-5 |
| _version_ | 1855484141994246144 |
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| author | Goytia Bertero, Valentina Beznec, Ailin Faccio, Paula Daniela Auteri, Micol Tais Arteaga, Martin Bonafede, Marcos Bossio, Maria Emilia |
| author_browse | Arteaga, Martin Auteri, Micol Tais Beznec, Ailin Bonafede, Marcos Bossio, Maria Emilia Faccio, Paula Daniela Goytia Bertero, Valentina |
| author_facet | Goytia Bertero, Valentina Beznec, Ailin Faccio, Paula Daniela Auteri, Micol Tais Arteaga, Martin Bonafede, Marcos Bossio, Maria Emilia |
| author_sort | Goytia Bertero, Valentina |
| collection | INTA Digital |
| description | In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis). |
| format | Artículo |
| id | INTA8145 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA81452020-10-28T22:35:09Z High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) Goytia Bertero, Valentina Beznec, Ailin Faccio, Paula Daniela Auteri, Micol Tais Arteaga, Martin Bonafede, Marcos Bossio, Maria Emilia Minthostachys Somatic Embryogenesis In Vitro Culture Embriogénesis Somática Cultivo in Vitro Griseb Peperina In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and medicinal species, with applications in both industrial and academic laboratories. In this study, we describe somatic embryogenesis and plant regeneration protocol for “peperina” plants (Minthostachys verticillata (Griseb.) Epling). In vitro shoots developed via shoot apex extracted from greenhouse-grown plants were cultured on shoot elongation medium (ShM) consisting of Murashige and Skoog (MS) basal salts supplemented with myoinositol, thiamine, and benzyladenine. Shoot apexes were disinfected with 70% ethanol for 5 min and 0.26 sodium hypochlorite (w/v) for 5 min, before the initiation of in vitro culture. For somatic embryo (SE) induction, leaves collected from 2-mo-old in vitro raised shoots were cultured on MS basal medium supplemented with benzyladenine and two different concentrations of coconut water (CW) until SE developed. Using 2.5% CW-supplemented medium, 100% of cultured leaves developed SE and 89.3% of the leaf explants developed plantlets. The resulting embryos germinated on the same medium, and the plantlets obtained were transferred onto ShM until they developed 3 to 4 leaves. To increase the low root development, shoots were transferred into fully hydrated perlite for rooting and then transplanted into soil-perlite containing pots for hardening. This protocol provides the first step to supply the demand and simultaneously protect the natural populations of Minthostachys verticillata from overexploitation. Furthermore, this protocol provides the first step for crop improvement by genetic modification (editing or transgenesis). Fil: Goytia Bertero, Valentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Arteaga, Martín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Auteri, Micol Tais. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Ingeniería Genética; Argentina 2020-10-28T22:16:35Z 2020-10-28T22:16:35Z 2020-07-30 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8145 https://link.springer.com/article/10.1007%2Fs11627-020-10098-5 1054-5476 https://doi.org/10.1007/s11627-020-10098-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer In Vitro Cellular & Developmental Biology - Plant (2020) |
| spellingShingle | Minthostachys Somatic Embryogenesis In Vitro Culture Embriogénesis Somática Cultivo in Vitro Griseb Peperina Goytia Bertero, Valentina Beznec, Ailin Faccio, Paula Daniela Auteri, Micol Tais Arteaga, Martin Bonafede, Marcos Bossio, Maria Emilia High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title | High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title_full | High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title_fullStr | High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title_full_unstemmed | High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title_short | High - efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of "peperina" (Minthostachys verticillata) |
| title_sort | high efficiency direst somatic embryogenesis and plant regeneration from leaf base plants of peperina minthostachys verticillata |
| topic | Minthostachys Somatic Embryogenesis In Vitro Culture Embriogénesis Somática Cultivo in Vitro Griseb Peperina |
| url | http://hdl.handle.net/20.500.12123/8145 https://link.springer.com/article/10.1007%2Fs11627-020-10098-5 https://doi.org/10.1007/s11627-020-10098-5 |
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