Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina
Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed t...
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| Format: | Artículo |
| Language: | Inglés |
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Springer Verlag
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8143 https://link.springer.com/article/10.1007/s00436-020-06922-x https://doi.org/10.1007/s00436-020-06922-x |
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| author | Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina |
| author_browse | Canton, German Jose Dorsch, Matias Hecker, Yanina Moore, Prando Dadin Morrell, Eleonora Lidia Ortega-Mora, Luis Regidor-Cerrillo, Javier Scioli, Maria Valeria |
| author_facet | Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina |
| author_sort | Dorsch, Matias |
| collection | INTA Digital |
| description | Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
| format | Artículo |
| id | INTA8143 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Verlag |
| publisherStr | Springer Verlag |
| record_format | dspace |
| spelling | INTA81432020-10-28T21:49:25Z Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008–2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. EEA Balcarce Fil: Dorsch, Matías A. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Investigación Agropecuaria (INIA); Uruguay. Fil: Regidor-Cerrillo, Javier. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Ortega-Mora, Luis. Universidad Complutense de Madrid. Facultad de Ciencias Veterinarias; España. Fil: Scioli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morrel, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cantón, Germán Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Moore, Dadín. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Hercker, Yanina. IPADS Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2020-10-28T21:39:08Z 2020-10-28T21:39:08Z 2020-10-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8143 https://link.springer.com/article/10.1007/s00436-020-06922-x 1432-1955 0932-0113 https://doi.org/10.1007/s00436-020-06922-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Verlag Parasitology Research (2020) |
| spellingShingle | Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda Dorsch, Matias Regidor-Cerrillo, Javier Ortega-Mora, Luis Scioli, Maria Valeria Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Hecker, Yanina Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title_full | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title_fullStr | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title_full_unstemmed | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title_short | Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina |
| title_sort | microsatellite genotyping reveals extensive genetic diversity in bovine neospora caninum from the humid pampa region in argentina |
| topic | Bovina Neospora Caninum Aborto Microsatélites Argentina Bovinae Abortion Microsatellites Región Pampa Húmeda |
| url | http://hdl.handle.net/20.500.12123/8143 https://link.springer.com/article/10.1007/s00436-020-06922-x https://doi.org/10.1007/s00436-020-06922-x |
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