Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity
Caprine brucellosis is an infectious, contagious zoonotic disease caused by Brucella melitensis. Multiple factors, including host genetics, can influence the outcome of the exposure to Brucella; and it is expected that genetic variants that affect the host innate immune response could have a key rol...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8136 https://www.sciencedirect.com/science/article/pii/S088875432030269X https://doi.org/10.1016/j.ygeno.2020.06.050 |
| _version_ | 1855035981956120576 |
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| author | Hasenauer, Flavia Carolina Rossi, Ursula Caffaro, María Eugenia Raschia, Maria Agustina Maurizio, Estefanía Poli, Mario Andres Rossetti, Carlos Alberto |
| author_browse | Caffaro, María Eugenia Hasenauer, Flavia Carolina Maurizio, Estefanía Poli, Mario Andres Raschia, Maria Agustina Rossetti, Carlos Alberto Rossi, Ursula |
| author_facet | Hasenauer, Flavia Carolina Rossi, Ursula Caffaro, María Eugenia Raschia, Maria Agustina Maurizio, Estefanía Poli, Mario Andres Rossetti, Carlos Alberto |
| author_sort | Hasenauer, Flavia Carolina |
| collection | INTA Digital |
| description | Caprine brucellosis is an infectious, contagious zoonotic disease caused by Brucella melitensis. Multiple factors, including host genetics, can influence the outcome of the exposure to Brucella; and it is expected that genetic variants that affect the host innate immune response could have a key role in Brucella infection and pathogenesis. In this study, we evaluated if polymorphisms in innate immunity-related genes are associated with results of Brucella infection in goats. Nine polymorphisms within interferon gamma (IFNG), tumor necrosis factor (TNF), MyD88 innate immune signal transduction adaptor (MYD88), interleukin 10 (IL10) and IL-10 receptor subunit alpha (IL10RA) genes and two molecular markers (BMS2753 and INRA111) were resolved by PCR-capillary electrophoresis in samples from 81 seronegative and 61 seropositive goats for brucellosis. A heterozygous genotype at INRA111, a microsatellite near the VRK serine/threonine kinase 2 (VRK2) gene, was associated with absence of Brucella-specific antibodies in goats naturally exposed to the pathogen (P = .004). Conversely, variants in the TNF gene (rs668920841) and near the IFN gamma receptor 1 (IFNGR1) gene (microsatellite BMS2753) were significantly associated with presence of Brucella-specific antibodies at allelic (P = .042 and P = .046) and genotypic level (P = .012 and P = .041, respectively). Moreover, an in silico analysis predicted a functional role of the insertion-deletion polymorphism rs668920841 on the transcriptional regulation of the caprine TNF gene. Altogether, these results contribute to the identification of genetic factors that have a putative effect on the resistance / susceptibility phenotype of goats to Brucella infection. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8136 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA81362020-10-27T17:15:37Z Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity Hasenauer, Flavia Carolina Rossi, Ursula Caffaro, María Eugenia Raschia, Maria Agustina Maurizio, Estefanía Poli, Mario Andres Rossetti, Carlos Alberto Genotypes Genetic Markers Goats Polymorphism Brucellosis Immunity Genotipos Marcadores Genéticos Caprinos Polimorfismo Brucelosis Inmunidad Candidate Genes Innate Immunity Genes Candidatos Inmunidad Innata Caprine brucellosis is an infectious, contagious zoonotic disease caused by Brucella melitensis. Multiple factors, including host genetics, can influence the outcome of the exposure to Brucella; and it is expected that genetic variants that affect the host innate immune response could have a key role in Brucella infection and pathogenesis. In this study, we evaluated if polymorphisms in innate immunity-related genes are associated with results of Brucella infection in goats. Nine polymorphisms within interferon gamma (IFNG), tumor necrosis factor (TNF), MyD88 innate immune signal transduction adaptor (MYD88), interleukin 10 (IL10) and IL-10 receptor subunit alpha (IL10RA) genes and two molecular markers (BMS2753 and INRA111) were resolved by PCR-capillary electrophoresis in samples from 81 seronegative and 61 seropositive goats for brucellosis. A heterozygous genotype at INRA111, a microsatellite near the VRK serine/threonine kinase 2 (VRK2) gene, was associated with absence of Brucella-specific antibodies in goats naturally exposed to the pathogen (P = .004). Conversely, variants in the TNF gene (rs668920841) and near the IFN gamma receptor 1 (IFNGR1) gene (microsatellite BMS2753) were significantly associated with presence of Brucella-specific antibodies at allelic (P = .042 and P = .046) and genotypic level (P = .012 and P = .041, respectively). Moreover, an in silico analysis predicted a functional role of the insertion-deletion polymorphism rs668920841 on the transcriptional regulation of the caprine TNF gene. Altogether, these results contribute to the identification of genetic factors that have a putative effect on the resistance / susceptibility phenotype of goats to Brucella infection. Instituto de Patobiología Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2020-10-27T17:07:55Z 2020-10-27T17:07:55Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8136 https://www.sciencedirect.com/science/article/pii/S088875432030269X 0888-7543 https://doi.org/10.1016/j.ygeno.2020.06.050 eng info:eu-repograntAgreement/INTA/PNBIO-1131033/AR./Genómica y biotecnología aplicada a la cría animal. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Genomics 112 (6) : 3925-3932 (Noviembre 2020) |
| spellingShingle | Genotypes Genetic Markers Goats Polymorphism Brucellosis Immunity Genotipos Marcadores Genéticos Caprinos Polimorfismo Brucelosis Inmunidad Candidate Genes Innate Immunity Genes Candidatos Inmunidad Innata Hasenauer, Flavia Carolina Rossi, Ursula Caffaro, María Eugenia Raschia, Maria Agustina Maurizio, Estefanía Poli, Mario Andres Rossetti, Carlos Alberto Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title | Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title_full | Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title_fullStr | Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title_full_unstemmed | Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title_short | Association of TNF rs668920841 and INRA111 polymorphisms with caprine brucellosis: A case-control study of candidate genes involved in innate immunity |
| title_sort | association of tnf rs668920841 and inra111 polymorphisms with caprine brucellosis a case control study of candidate genes involved in innate immunity |
| topic | Genotypes Genetic Markers Goats Polymorphism Brucellosis Immunity Genotipos Marcadores Genéticos Caprinos Polimorfismo Brucelosis Inmunidad Candidate Genes Innate Immunity Genes Candidatos Inmunidad Innata |
| url | http://hdl.handle.net/20.500.12123/8136 https://www.sciencedirect.com/science/article/pii/S088875432030269X https://doi.org/10.1016/j.ygeno.2020.06.050 |
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