A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides
Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards m...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/8135 https://www.mdpi.com/1999-4915/12/10/1161 https://doi.org/10.3390/v12101161 |
| _version_ | 1855484140074303488 |
|---|---|
| author | Jacquat, Andrés Gustavo Theumer, Martín Gustavo Cañizares, María Carmen Debat, Humberto Julio Iglesias, Juliana García Pedrajas, María Dolores Dambolena, José Sebastián |
| author_browse | Cañizares, María Carmen Dambolena, José Sebastián Debat, Humberto Julio García Pedrajas, María Dolores Iglesias, Juliana Jacquat, Andrés Gustavo Theumer, Martín Gustavo |
| author_facet | Jacquat, Andrés Gustavo Theumer, Martín Gustavo Cañizares, María Carmen Debat, Humberto Julio Iglesias, Juliana García Pedrajas, María Dolores Dambolena, José Sebastián |
| author_sort | Jacquat, Andrés Gustavo |
| collection | INTA Digital |
| description | Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring. |
| format | Artículo |
| id | INTA8135 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA81352020-10-27T17:06:35Z A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides Jacquat, Andrés Gustavo Theumer, Martín Gustavo Cañizares, María Carmen Debat, Humberto Julio Iglesias, Juliana García Pedrajas, María Dolores Dambolena, José Sebastián Maíz Gramíneas Forrajeras Sorgos Hongos Fusarium Maize Feed Grasses Sorghum Grain Fungi Mitovirus Mycovirus Fusarium verticillioides Fusarium andiyazi Mycoviruses appear to be widespread in Fusarium species worldwide. The aim of this work was to identify mycoviral infections in Fusarium spp., isolated from maize and sorghum grown in Argentina, and to estimate their potential effects on the pathogenicity and toxigenesis of the host fungus towards maize. Mycoviruses were identified in 2 out of 105 isolates analyzed; Fusarium verticillioides strain Sec505 and Fusarium andiyazi strain 162. They were characterized as members of the genus Mitovirus by high-throughput sequencing and sequence analysis. The F. verticillioides mitovirus was a novel mycovirus whereas the F. andiyazi mitovirus was found to be a new strain of a previously identified mitovirus. We have named these mitoviruses, Fusarium verticillioides mitovirus 1 (FvMV1) and Fusarium andiyazi mitovirus 1 strain 162 (FaMV1-162). To our knowledge, FvMV1 is the first mycovirus reported as naturally infecting F. verticillioides, the major causal agent of ear rot and fumonisin producer in corn. Both mitoviruses exhibited 100% vertical transmission rate to microconidia. The Fa162 strain infected with FaMV1-162 did not show phenotypic alterations. In contract, F. verticillioides Sec505 infected with FvMV1 showed increased virulence as well as microconidia and fumonisin-B1 production, compared with two uninfected strains. These results suggest that FvMV1 could have a role in modulating F. verticillioides pathogenicity and toxin production worth further exploring. EEA Pergamino Fil: Jacquat, Andrés Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Theumer, Martín Gustavo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas . Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Cañizares, María Carmen. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM — UMA — CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino. Departamento de Maíz; Argentina. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Escuela de Agrarias, Naturales y Ambientales; Argentina Fil: García Pedrajas, María Dolores. Universidad de Málaga. Consejo Superior de Investigaciones Científicas (IHSM - UMA - CSIC). Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”; España Fil: Dambolena, José Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina 2020-10-27T16:11:06Z 2020-10-27T16:11:06Z 2020-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8135 https://www.mdpi.com/1999-4915/12/10/1161 1999-4915 https://doi.org/10.3390/v12101161 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Viruses 12 (10) : 1161 (October 2020) |
| spellingShingle | Maíz Gramíneas Forrajeras Sorgos Hongos Fusarium Maize Feed Grasses Sorghum Grain Fungi Mitovirus Mycovirus Fusarium verticillioides Fusarium andiyazi Jacquat, Andrés Gustavo Theumer, Martín Gustavo Cañizares, María Carmen Debat, Humberto Julio Iglesias, Juliana García Pedrajas, María Dolores Dambolena, José Sebastián A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title | A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title_full | A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title_fullStr | A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title_full_unstemmed | A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title_short | A Survey of Mycoviral Infection in Fusarium spp. Isolated from Maize and Sorghum in Argentina Identifies the First Mycovirus from Fusarium verticillioides |
| title_sort | survey of mycoviral infection in fusarium spp isolated from maize and sorghum in argentina identifies the first mycovirus from fusarium verticillioides |
| topic | Maíz Gramíneas Forrajeras Sorgos Hongos Fusarium Maize Feed Grasses Sorghum Grain Fungi Mitovirus Mycovirus Fusarium verticillioides Fusarium andiyazi |
| url | http://hdl.handle.net/20.500.12123/8135 https://www.mdpi.com/1999-4915/12/10/1161 https://doi.org/10.3390/v12101161 |
| work_keys_str_mv | AT jacquatandresgustavo asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT theumermartingustavo asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT canizaresmariacarmen asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT debathumbertojulio asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT iglesiasjuliana asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT garciapedrajasmariadolores asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT dambolenajosesebastian asurveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT jacquatandresgustavo surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT theumermartingustavo surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT canizaresmariacarmen surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT debathumbertojulio surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT iglesiasjuliana surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT garciapedrajasmariadolores surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides AT dambolenajosesebastian surveyofmycoviralinfectioninfusariumsppisolatedfrommaizeandsorghuminargentinaidentifiesthefirstmycovirusfromfusariumverticillioides |