Protocolo de PCR para la detección de Brucella melitensis y REV-1

Primers utilizados: REV Fw 5’ CAG GCA AAC CCT CAG AAG C 3’ REV Rv 5’ GAT GTG GTA ACG CAC ACC AA 3’ Amplifican un de 218 pb en REV-1. Cita: Garcia-Yoldi, D., et al., Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19...

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Bibliographic Details
Main Authors: Alvarez, Lucia Paula, Robles, Carlos Alejandro
Format: info:ar-repo/semantics/informe técnico
Language:Español
Published: EEA Bariloche 2020
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/8083
Description
Summary:Primers utilizados: REV Fw 5’ CAG GCA AAC CCT CAG AAG C 3’ REV Rv 5’ GAT GTG GTA ACG CAC ACC AA 3’ Amplifican un de 218 pb en REV-1. Cita: Garcia-Yoldi, D., et al., Multiplex PCR assay for the identification and differentiation of all Brucella species and the vaccine strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1. Clin Chem, 2006. 52(4): p. 779-81.