A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina
Specimens of a Trypanosoma sp. were found in a haemolymph sample of Rhipicephalus microplus from Argentina. Polymerase chain reaction (PCR) was done targeting the SSU rRNA gene of Trypanosoma spp. and a fragment of 2300 base pairs (bp) was amplified, subsequently a phylogenetic analysis was conducte...
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8078 https://www.sciencedirect.com/science/article/pii/S1877959X20304428 https://doi.org/10.1016/j.ttbdis.2020.101573 |
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| author | Morel, Nicolas Thompson, Carolina Soledad Rossner, Maria Victoria Mangold, Atilio Jose Nava, Santiago |
| author_browse | Mangold, Atilio Jose Morel, Nicolas Nava, Santiago Rossner, Maria Victoria Thompson, Carolina Soledad |
| author_facet | Morel, Nicolas Thompson, Carolina Soledad Rossner, Maria Victoria Mangold, Atilio Jose Nava, Santiago |
| author_sort | Morel, Nicolas |
| collection | INTA Digital |
| description | Specimens of a Trypanosoma sp. were found in a haemolymph sample of Rhipicephalus microplus from Argentina. Polymerase chain reaction (PCR) was done targeting the SSU rRNA gene of Trypanosoma spp. and a fragment of 2300 base pairs (bp) was amplified, subsequently a phylogenetic analysis was conducted, based on an alignment of 905 bp, containing the sequence of the Argentina isolate and sequences of different Trypanosoma species retrieved from GenBank. Phylogenetic analysis revealed that this trypanosome is not related to Trypanosoma theileri as was previously thought, instead the strain of Trypanosoma detected in this study can be provisionally determined as belonging to the recently described organism Trypanosoma rhipicephalis. Furthermore, phylogenetic analysis performed in this work revealed that T. rhipicephalis belongs to a novel clade of tick-related trypanosomes, most with limited genetic data, for which essential aspects of both the vertebrate and invertebrate life cycles are lacking. The lack of basic information restricts the inferences that can be done from the present finding and, in addition, points out a clear knowledge gap in the biology of this group of trypanosomes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8078 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA80782020-10-19T14:52:37Z A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina Morel, Nicolas Thompson, Carolina Soledad Rossner, Maria Victoria Mangold, Atilio Jose Nava, Santiago Trypanosoma Rhipicephalus Identificación Ganado Bovino Argentina Identification Cattle Rhipicephalus (Boophilus) microplus Garrapatas Ticks Specimens of a Trypanosoma sp. were found in a haemolymph sample of Rhipicephalus microplus from Argentina. Polymerase chain reaction (PCR) was done targeting the SSU rRNA gene of Trypanosoma spp. and a fragment of 2300 base pairs (bp) was amplified, subsequently a phylogenetic analysis was conducted, based on an alignment of 905 bp, containing the sequence of the Argentina isolate and sequences of different Trypanosoma species retrieved from GenBank. Phylogenetic analysis revealed that this trypanosome is not related to Trypanosoma theileri as was previously thought, instead the strain of Trypanosoma detected in this study can be provisionally determined as belonging to the recently described organism Trypanosoma rhipicephalis. Furthermore, phylogenetic analysis performed in this work revealed that T. rhipicephalis belongs to a novel clade of tick-related trypanosomes, most with limited genetic data, for which essential aspects of both the vertebrate and invertebrate life cycles are lacking. The lack of basic information restricts the inferences that can be done from the present finding and, in addition, points out a clear knowledge gap in the biology of this group of trypanosomes. EEA Rafaela Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Thompson, Carolina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-10-19T14:45:40Z 2020-10-19T14:45:40Z 2020-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8078 https://www.sciencedirect.com/science/article/pii/S1877959X20304428 1877-959X https://doi.org/10.1016/j.ttbdis.2020.101573 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Ticks and Tick-borne Diseases 12 (1) : 101573 (January 2021) |
| spellingShingle | Trypanosoma Rhipicephalus Identificación Ganado Bovino Argentina Identification Cattle Rhipicephalus (Boophilus) microplus Garrapatas Ticks Morel, Nicolas Thompson, Carolina Soledad Rossner, Maria Victoria Mangold, Atilio Jose Nava, Santiago A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title | A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_full | A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_fullStr | A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_full_unstemmed | A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_short | A Trypanosoma species detected in Rhipicephalus (Boophilus) microplus ticks from Argentina |
| title_sort | trypanosoma species detected in rhipicephalus boophilus microplus ticks from argentina |
| topic | Trypanosoma Rhipicephalus Identificación Ganado Bovino Argentina Identification Cattle Rhipicephalus (Boophilus) microplus Garrapatas Ticks |
| url | http://hdl.handle.net/20.500.12123/8078 https://www.sciencedirect.com/science/article/pii/S1877959X20304428 https://doi.org/10.1016/j.ttbdis.2020.101573 |
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