In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina
The aim of this work was to adapt and apply the in vitro larval bioassay packet test (LPT) to diagnose resistance of Rhipicephalus microplus ticks to fipronil in Argentina. Ten serial dilutions of fipronil were included in the LPT bioassay. Seven populations of R. microplus from six Argentinean Prov...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8077 https://link.springer.com/article/10.1007/s10493-020-00554-7 https://doi.org/10.1007/s10493-020-00554-7 |
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| author | Torrents, Jorgelina Morel, Nicolas Rossner, Maria Victoria Martinez, Norberto Claudio Toffaletti, Jose Rodolfo Nava, Santiago |
| author_browse | Martinez, Norberto Claudio Morel, Nicolas Nava, Santiago Rossner, Maria Victoria Toffaletti, Jose Rodolfo Torrents, Jorgelina |
| author_facet | Torrents, Jorgelina Morel, Nicolas Rossner, Maria Victoria Martinez, Norberto Claudio Toffaletti, Jose Rodolfo Nava, Santiago |
| author_sort | Torrents, Jorgelina |
| collection | INTA Digital |
| description | The aim of this work was to adapt and apply the in vitro larval bioassay packet test (LPT) to diagnose resistance of Rhipicephalus microplus ticks to fipronil in Argentina. Ten serial dilutions of fipronil were included in the LPT bioassay. Seven populations of R. microplus from six Argentinean Provinces were evaluated. Samples from Colonia Benítez, Quimilí and Intiyaco were unambiguously classified in the category ‘resistant’. In all these cases the 95% confidence intervals did not overlap with that of the susceptible reference strain and the slope values were lower than the slope value obtained for the susceptible reference strain. The resistance ratio (RR50) values of the populations from Colonia Benítez I and II, Quimilí and Intiyaco were 2.23, 3.49, 6.84 and 3.39, respectively. Populations from El Colorado I and II, Acambuco, Vera and Feliciano were classified as ‘susceptible’ because the 95% confidence intervals overlapped with that of the susceptible reference strain and the RR50 values never exceeded 1.32. The results of these LPT bioassays indicate the presence of fipronil resistant R. microplus populations in Argentina. |
| format | Artículo |
| id | INTA8077 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA80772020-10-19T14:25:05Z In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina Torrents, Jorgelina Morel, Nicolas Rossner, Maria Victoria Martinez, Norberto Claudio Toffaletti, Jose Rodolfo Nava, Santiago Rhipicephalus Control de Plagas Ganado Bovino Fipronil Acaricidas Argentina Experimentación in Vitro Pest Control Cattle Acaricides In Vitro Experimentation Garrapatas Rhipicephalus (Boophilus) microplus Ticks The aim of this work was to adapt and apply the in vitro larval bioassay packet test (LPT) to diagnose resistance of Rhipicephalus microplus ticks to fipronil in Argentina. Ten serial dilutions of fipronil were included in the LPT bioassay. Seven populations of R. microplus from six Argentinean Provinces were evaluated. Samples from Colonia Benítez, Quimilí and Intiyaco were unambiguously classified in the category ‘resistant’. In all these cases the 95% confidence intervals did not overlap with that of the susceptible reference strain and the slope values were lower than the slope value obtained for the susceptible reference strain. The resistance ratio (RR50) values of the populations from Colonia Benítez I and II, Quimilí and Intiyaco were 2.23, 3.49, 6.84 and 3.39, respectively. Populations from El Colorado I and II, Acambuco, Vera and Feliciano were classified as ‘susceptible’ because the 95% confidence intervals overlapped with that of the susceptible reference strain and the RR50 values never exceeded 1.32. The results of these LPT bioassays indicate the presence of fipronil resistant R. microplus populations in Argentina. EEA Rafaela Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Martinez, Norberto Claudio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista. Agencia De Extensión Rural Garabato; Argentina Fil: Toffaletti, Jose Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-10-19T14:20:54Z 2020-10-19T14:20:54Z 2020-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8077 https://link.springer.com/article/10.1007/s10493-020-00554-7 0168-8162 1572-9702 https://doi.org/10.1007/s10493-020-00554-7 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Experimental and Applied Acarology (2020) |
| spellingShingle | Rhipicephalus Control de Plagas Ganado Bovino Fipronil Acaricidas Argentina Experimentación in Vitro Pest Control Cattle Acaricides In Vitro Experimentation Garrapatas Rhipicephalus (Boophilus) microplus Ticks Torrents, Jorgelina Morel, Nicolas Rossner, Maria Victoria Martinez, Norberto Claudio Toffaletti, Jose Rodolfo Nava, Santiago In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title | In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title_full | In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title_fullStr | In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title_full_unstemmed | In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title_short | In vitro diagnosis of resistance of the cattle tick Rhipicephalus (Boophilus) microplus to fipronil in Argentina |
| title_sort | in vitro diagnosis of resistance of the cattle tick rhipicephalus boophilus microplus to fipronil in argentina |
| topic | Rhipicephalus Control de Plagas Ganado Bovino Fipronil Acaricidas Argentina Experimentación in Vitro Pest Control Cattle Acaricides In Vitro Experimentation Garrapatas Rhipicephalus (Boophilus) microplus Ticks |
| url | http://hdl.handle.net/20.500.12123/8077 https://link.springer.com/article/10.1007/s10493-020-00554-7 https://doi.org/10.1007/s10493-020-00554-7 |
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