Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations
In this study, we sequenced two complete mitochondrial genomes of Amblyomma ovale, a tick of public health importance. Sequencing two distinct individuals, the resulting mitochondrial genomes were 14,756 and 14,760 bp in length and maintained the same gene order previously reported in Amblyomma. The...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8056 https://link.springer.com/article/10.1007/s10493-020-00512-3 https://doi.org/10.1007/s10493-020-00512-3 |
| _version_ | 1855484125930061824 |
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| author | Uribe, Juan E. Nava, Santiago Murphy, Katherine R. Tarragona, Evelina Luisa Castro, Lyda R. |
| author_browse | Castro, Lyda R. Murphy, Katherine R. Nava, Santiago Tarragona, Evelina Luisa Uribe, Juan E. |
| author_facet | Uribe, Juan E. Nava, Santiago Murphy, Katherine R. Tarragona, Evelina Luisa Castro, Lyda R. |
| author_sort | Uribe, Juan E. |
| collection | INTA Digital |
| description | In this study, we sequenced two complete mitochondrial genomes of Amblyomma ovale, a tick of public health importance. Sequencing two distinct individuals, the resulting mitochondrial genomes were 14,756 and 14,760 bp in length and maintained the same gene order previously reported in Amblyomma. These were combined with RNA-seq derived mitochondrial sequences from three additional species, Amblyomma aureolatum, Amblyomma maculatum, and Amblyomma moreliae, to carry out mitogenome comparative and evolutionary analyses against all previously published tick mitochondrial genomes. We described a derivative genome rearrangement that isolates Ixodes from the remaining Ixodidae and consists of both a reverse translocation as well as an event of Tandem Duplication Random Loss. Genetic distance analyses indicated that cox2, nd1, nd5, and 16S are good candidates for future population studies in A. ovale. The phylogenetic analyses corroborated the utility of complete mitochondrial genomes as phylogenetic markers within the group. This study further supplements the genome information available for Amblyomma and facilitates future evolutionary and population genetic studies within the genus. |
| format | Artículo |
| id | INTA8056 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA80562020-10-15T12:33:01Z Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations Uribe, Juan E. Nava, Santiago Murphy, Katherine R. Tarragona, Evelina Luisa Castro, Lyda R. Amblyomma Genomas Filogenia Marcadores Genéticos Genomes Phylogeny Genetic Markers Garrapatas Amblyomma ovale Marcadores Moleculares Ticks In this study, we sequenced two complete mitochondrial genomes of Amblyomma ovale, a tick of public health importance. Sequencing two distinct individuals, the resulting mitochondrial genomes were 14,756 and 14,760 bp in length and maintained the same gene order previously reported in Amblyomma. These were combined with RNA-seq derived mitochondrial sequences from three additional species, Amblyomma aureolatum, Amblyomma maculatum, and Amblyomma moreliae, to carry out mitogenome comparative and evolutionary analyses against all previously published tick mitochondrial genomes. We described a derivative genome rearrangement that isolates Ixodes from the remaining Ixodidae and consists of both a reverse translocation as well as an event of Tandem Duplication Random Loss. Genetic distance analyses indicated that cox2, nd1, nd5, and 16S are good candidates for future population studies in A. ovale. The phylogenetic analyses corroborated the utility of complete mitochondrial genomes as phylogenetic markers within the group. This study further supplements the genome information available for Amblyomma and facilitates future evolutionary and population genetic studies within the genus. EEA Rafaela Fil: Uribe, Juan E. Museo Nacional de Ciencias Naturales. Department of Biodiversity and Evolutionary Biology; España. Smithsonian Institution. National Museum of Natural History. Department of Invertebrate Zoology; Estados Unidos Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Murphy, Katherine R. Smithsonian Institution. National Museum of Natural History. Laboratories of Analytical Biology; Estados Unidos Fil: Tarragona, Evelina Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Castro, Lyda R. Universidad del Magdalena. Grupo de Investigación Evolución, Sistemática y Ecología Molecular; Colombia 2020-10-15T12:28:51Z 2020-10-15T12:28:51Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8056 https://link.springer.com/article/10.1007/s10493-020-00512-3 0168-8162 1572-9702 https://doi.org/10.1007/s10493-020-00512-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Experimental and Applied Acarology 81 : 421–439, (2020) |
| spellingShingle | Amblyomma Genomas Filogenia Marcadores Genéticos Genomes Phylogeny Genetic Markers Garrapatas Amblyomma ovale Marcadores Moleculares Ticks Uribe, Juan E. Nava, Santiago Murphy, Katherine R. Tarragona, Evelina Luisa Castro, Lyda R. Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title | Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title_full | Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title_fullStr | Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title_full_unstemmed | Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title_short | Characterization of the complete mitochondrial genome of Amblyomma ovale, comparative analyses and phylogenetic considerations |
| title_sort | characterization of the complete mitochondrial genome of amblyomma ovale comparative analyses and phylogenetic considerations |
| topic | Amblyomma Genomas Filogenia Marcadores Genéticos Genomes Phylogeny Genetic Markers Garrapatas Amblyomma ovale Marcadores Moleculares Ticks |
| url | http://hdl.handle.net/20.500.12123/8056 https://link.springer.com/article/10.1007/s10493-020-00512-3 https://doi.org/10.1007/s10493-020-00512-3 |
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