Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America
To obtain information about rickettsial bacteria in ticks of the genus Ixodes from Argentina and Uruguay, specimens of I. fuscipes (previously named as I. aragaoi), Ixodes pararicinus, Ixodes sp. cf. I. affinis, and Ixodes sp. were tested targeting the rickettsial gltA and ompA genes. Rickettsial ba...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8049 https://www.sciencedirect.com/science/article/abs/pii/S0001706X19318108 https://doi.org/10.1016/j.actatropica.2020.105588 |
| _version_ | 1855484124739928064 |
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| author | Sebastian, Patrick Stephan Flores, Fernando Sebastian Saracho Bottero, Maria Noelia Tarragona, Evelina Luisa Venzal, José Manuel Nava, Santiago |
| author_browse | Flores, Fernando Sebastian Nava, Santiago Saracho Bottero, Maria Noelia Sebastian, Patrick Stephan Tarragona, Evelina Luisa Venzal, José Manuel |
| author_facet | Sebastian, Patrick Stephan Flores, Fernando Sebastian Saracho Bottero, Maria Noelia Tarragona, Evelina Luisa Venzal, José Manuel Nava, Santiago |
| author_sort | Sebastian, Patrick Stephan |
| collection | INTA Digital |
| description | To obtain information about rickettsial bacteria in ticks of the genus Ixodes from Argentina and Uruguay, specimens of I. fuscipes (previously named as I. aragaoi), Ixodes pararicinus, Ixodes sp. cf. I. affinis, and Ixodes sp. were tested targeting the rickettsial gltA and ompA genes. Rickettsial bacteria was detected in all of these species. Rickettsia found in Ixodes sp. is closely related to Ca. Rickettsia mendelii, which was previously detected in I. ricinus of the Czech Republic and later in I. brunneus from the USA. Phylogenetic analyses of the Rickettsia strains found in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis showed that these strains form a clade together with R. buchneri detected in I. scapularis from the USA, which is closely related to R. monacensis from Europe. Rickettsia buchneri, R. cooleyi and the Rickettsia detected in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis clustered together in a clade well supported, which suggest that they are different strains of R. buchneri. The phylogenetic analysis shows that Ixodes ticks that are closely related in evolutionary terms (i.e. Ixodes species from the I. ricinus complex, I. brunneus-Ixodes sp.) share closely related rickettsial strains. The results of this study show that rickettsial bacteria are present in Ixodes ticks from Argentina and describe the first detection of Ca. R. mendelii in South America. |
| format | Artículo |
| id | INTA8049 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA80492020-10-14T12:56:24Z Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America Sebastian, Patrick Stephan Flores, Fernando Sebastian Saracho Bottero, Maria Noelia Tarragona, Evelina Luisa Venzal, José Manuel Nava, Santiago Ixodes Rickettsia Enfermedades Transmitidas por Garrapatas América del Sur Tick-borne Diseases South America Garrapatas Rickettsia mendelii Ticks To obtain information about rickettsial bacteria in ticks of the genus Ixodes from Argentina and Uruguay, specimens of I. fuscipes (previously named as I. aragaoi), Ixodes pararicinus, Ixodes sp. cf. I. affinis, and Ixodes sp. were tested targeting the rickettsial gltA and ompA genes. Rickettsial bacteria was detected in all of these species. Rickettsia found in Ixodes sp. is closely related to Ca. Rickettsia mendelii, which was previously detected in I. ricinus of the Czech Republic and later in I. brunneus from the USA. Phylogenetic analyses of the Rickettsia strains found in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis showed that these strains form a clade together with R. buchneri detected in I. scapularis from the USA, which is closely related to R. monacensis from Europe. Rickettsia buchneri, R. cooleyi and the Rickettsia detected in I. fuscipes, I. pararicinus, and Ixodes sp. cf. I. affinis clustered together in a clade well supported, which suggest that they are different strains of R. buchneri. The phylogenetic analysis shows that Ixodes ticks that are closely related in evolutionary terms (i.e. Ixodes species from the I. ricinus complex, I. brunneus-Ixodes sp.) share closely related rickettsial strains. The results of this study show that rickettsial bacteria are present in Ixodes ticks from Argentina and describe the first detection of Ca. R. mendelii in South America. EEA Rafaela Fil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tarragona, Evelina Luisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria; Uruguay Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-10-14T12:53:37Z 2020-10-14T12:53:37Z 2020-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8049 https://www.sciencedirect.com/science/article/abs/pii/S0001706X19318108 0001-706X https://doi.org/10.1016/j.actatropica.2020.105588 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Acta Tropica 210 : 105588 (October 2020) |
| spellingShingle | Ixodes Rickettsia Enfermedades Transmitidas por Garrapatas América del Sur Tick-borne Diseases South America Garrapatas Rickettsia mendelii Ticks Sebastian, Patrick Stephan Flores, Fernando Sebastian Saracho Bottero, Maria Noelia Tarragona, Evelina Luisa Venzal, José Manuel Nava, Santiago Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title | Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title_full | Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title_fullStr | Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title_full_unstemmed | Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title_short | Molecular detection of rickettsial bacteria in ticks of the genus Ixodes from the Southern Cone of America |
| title_sort | molecular detection of rickettsial bacteria in ticks of the genus ixodes from the southern cone of america |
| topic | Ixodes Rickettsia Enfermedades Transmitidas por Garrapatas América del Sur Tick-borne Diseases South America Garrapatas Rickettsia mendelii Ticks |
| url | http://hdl.handle.net/20.500.12123/8049 https://www.sciencedirect.com/science/article/abs/pii/S0001706X19318108 https://doi.org/10.1016/j.actatropica.2020.105588 |
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