Isolation of streptomycetes causing common scab from 3-years old potato samples from South America
In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the pr...
| Autores principales: | , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Estonian Agricultural University
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8039 https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf https://doi.org/10.15159/ar.20.157 |
| _version_ | 1855035964949266432 |
|---|---|
| author | Rapoport, Daria Patrmanova, T. Kopecky, J. Mareckova, M. Clemente, Gladys Salvalaggio, Andrea Eugenia |
| author_browse | Clemente, Gladys Kopecky, J. Mareckova, M. Patrmanova, T. Rapoport, Daria Salvalaggio, Andrea Eugenia |
| author_facet | Rapoport, Daria Patrmanova, T. Kopecky, J. Mareckova, M. Clemente, Gladys Salvalaggio, Andrea Eugenia |
| author_sort | Rapoport, Daria |
| collection | INTA Digital |
| description | In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8039 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Estonian Agricultural University |
| publisherStr | Estonian Agricultural University |
| record_format | dspace |
| spelling | INTA80392020-10-13T18:01:06Z Isolation of streptomycetes causing common scab from 3-years old potato samples from South America Rapoport, Daria Patrmanova, T. Kopecky, J. Mareckova, M. Clemente, Gladys Salvalaggio, Andrea Eugenia Papa Streptomyces Scabies Enfermedades Bacterianas América del Sur Potatoes Bacterial Diseases South America Sarna Común In this paper, we aimed at assessing the best conditions for the isolation of actinobacteria from old potato samples. A set of media and pretreatments were tested. The optimal were chosen for the isolation of actinobacteria from potatoes from Peru, Chile and Argentina. Isolates were tested on the presence of thaxtomin phytotoxin by amplification of the respective gene. Phylogenetic position of strains was compared with their geographical origin, pathogenic potential and existence of common scab (CS) symptoms on potato sample. We demonstrated that RNAlater can be successfully used for the long-term preservation of potato peel for subsequent isolation of actinobacteria on R2A medium. Many streptomycetes were thaxtomin-positive, though they are distantly-related to described pathogens causing CS. Genus Nocardia was first reported to be thaxtomin-positive. Potentially pathogenic strains were isolated not only from infected potato but also from those lacking CS symptoms. Some strains from scabby potatoes were thaxtomin-negative. EEA Balcarce Fil: Rapoport, Daria. Charles University in Prague. Faculty opf Science, República Checa. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Fil: Patrmanova, T. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Fil: Kopecky, J. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Fil: Mareckova, M. Crop Research Institute, Laboratory of Epidemiology and Ecology of Microorganisms, República Checa. Czech University of Life Sciences, Faculty of Agrobiology, Food, and Natural Resources, Department of Microbiology, República Checa. Fil: Clemente, Gladys. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Salvalaggio, Andrea Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. 2020-10-13T17:53:55Z 2020-10-13T17:53:55Z 2020-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8039 https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf 1406-894X https://doi.org/10.15159/ar.20.157 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Estonian Agricultural University Agronomy Research 18 (S2) : 1433–1443 (2020) |
| spellingShingle | Papa Streptomyces Scabies Enfermedades Bacterianas América del Sur Potatoes Bacterial Diseases South America Sarna Común Rapoport, Daria Patrmanova, T. Kopecky, J. Mareckova, M. Clemente, Gladys Salvalaggio, Andrea Eugenia Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title | Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title_full | Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title_fullStr | Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title_full_unstemmed | Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title_short | Isolation of streptomycetes causing common scab from 3-years old potato samples from South America |
| title_sort | isolation of streptomycetes causing common scab from 3 years old potato samples from south america |
| topic | Papa Streptomyces Scabies Enfermedades Bacterianas América del Sur Potatoes Bacterial Diseases South America Sarna Común |
| url | http://hdl.handle.net/20.500.12123/8039 https://agronomy.emu.ee/wp-content/uploads/2020/06/AR2020_Vol18SI2_Rapoport.pdf https://doi.org/10.15159/ar.20.157 |
| work_keys_str_mv | AT rapoportdaria isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica AT patrmanovat isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica AT kopeckyj isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica AT mareckovam isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica AT clementegladys isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica AT salvalaggioandreaeugenia isolationofstreptomycetescausingcommonscabfrom3yearsoldpotatosamplesfromsouthamerica |