Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Royal Society of Chemistry
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7938 https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K https://doi.org/10.1039/D0CP01938K |
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| author | Pappalardo, Juan Sebastián Macairan, Jun-Ray Macina, Alexia Poulhazan, Alexandre Quattrocchi, Valeria Marcotte, Isabelle Naccache, Rafik |
| author_browse | Macairan, Jun-Ray Macina, Alexia Marcotte, Isabelle Naccache, Rafik Pappalardo, Juan Sebastián Poulhazan, Alexandre Quattrocchi, Valeria |
| author_facet | Pappalardo, Juan Sebastián Macairan, Jun-Ray Macina, Alexia Poulhazan, Alexandre Quattrocchi, Valeria Marcotte, Isabelle Naccache, Rafik |
| author_sort | Pappalardo, Juan Sebastián |
| collection | INTA Digital |
| description | Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen
content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly
remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapies |
| format | info:ar-repo/semantics/artículo |
| id | INTA7938 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Royal Society of Chemistry |
| publisherStr | Royal Society of Chemistry |
| record_format | dspace |
| spelling | INTA79382020-09-22T12:50:39Z Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo Pappalardo, Juan Sebastián Macairan, Jun-Ray Macina, Alexia Poulhazan, Alexandre Quattrocchi, Valeria Marcotte, Isabelle Naccache, Rafik Nanomedicina Nanotecnología Nanopartículas Nanomedicine Nanotechnology Nanoparticles Carbon Nanoparticles Nanopartículas Luminiscentes Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapies Estación Experimental Agropecuaria Bariloche Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Produccion Animal; Argentina Fil: Macairan, Jun-Ray. Concordia University. Department of Chemistry and Biochemistry; Canada Fil: Macina, Alexia. Concordia University. Department of Chemistry and Biochemistry; Canada Fil: Poulhazan, Alexandre. Universite du Quebec a Montreal. Department of Chemistry; Canada Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnologia Agropecuaria (INTA). Instituto de Virologia e Innovaciones Tecnológicas. Laboratorio de Bionanotecnología; Argentina Fil: Marcotte, Isabelle. Universite du Quebec a Montreal. Department of Chemistry; Canada Fil: Naccache, Rafik. Concordia University. Department of Chemistry and Biochemistry; Canada 2020-09-22T12:35:08Z 2020-09-22T12:35:08Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7938 https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K 1463-9084 https://doi.org/10.1039/D0CP01938K eng info:eu-repo/semantics/restrictedAccess application/pdf Royal Society of Chemistry Physical Chemistry Chemical Physics 22 : 16595 (2020) |
| spellingShingle | Nanomedicina Nanotecnología Nanopartículas Nanomedicine Nanotechnology Nanoparticles Carbon Nanoparticles Nanopartículas Luminiscentes Pappalardo, Juan Sebastián Macairan, Jun-Ray Macina, Alexia Poulhazan, Alexandre Quattrocchi, Valeria Marcotte, Isabelle Naccache, Rafik Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title | Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title_full | Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title_fullStr | Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title_full_unstemmed | Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title_short | Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo |
| title_sort | effects of polydopamine passivation on the optical properties of carbon dots and its potential use in vivo |
| topic | Nanomedicina Nanotecnología Nanopartículas Nanomedicine Nanotechnology Nanoparticles Carbon Nanoparticles Nanopartículas Luminiscentes |
| url | http://hdl.handle.net/20.500.12123/7938 https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K https://doi.org/10.1039/D0CP01938K |
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