Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies.
Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum sero...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7936 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302197 https://doi.org/10.1016/j.vprsr.2020.100438 |
| _version_ | 1855484102257410048 |
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| author | Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia |
| author_browse | Campero, Lucía María Dellarupe, Andrea Gual, Ignacio Moore, Prando Dadin Moré, Gastón Schares, Gereon Venturini, María Cecilia |
| author_facet | Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia |
| author_sort | Campero, Lucía María |
| collection | INTA Digital |
| description | Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina. |
| format | Artículo |
| id | INTA7936 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA79362020-09-22T12:18:33Z Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites Neospora caninum is an important abortifacient agent affecting mainly cattle worldwide. The aim of the present work was to describe the histopathological findings in a naturally infected beef cow and its midterm fetus caused by a genetically defined N. caninum isolate in Argentina. A N. caninum seropositive multiparous Aberdeen Angus pregnant cow and its fetus in the sixth month of gestation were submitted for histopathological, immunohistochemical, serological, and molecular studies and parasite isolation. The cow belonged to a beef herd under extensive management, with a N. caninum seroprevalence of 11%, and low level of annual abortion rate (≤ 5%). The dam had mild lymphocytic infiltrate in CNS, heart and uterus and no parasites were detected by Immunohistochemistry (IHC). No parasitic DNA was detected in the dam's brain, and gamma interferon knockout mice inoculated with brain material did not become infected. Clusters of tachyzoites and parasitic DNA were detected in the placenta by IHC and PCR, respectively. However, isolation from the placenta was unsuccessful. The fetus developed specific antibodies and an inflammatory response was detected in multiple organs. Furthermore, in vitro and in vivo isolation was achieved from gamma interferon knockout mice inoculated with CNS from the fetus. Multilocus-microsatellite typing revealed a genetically defined N. caninum isolate similar to the previously reported as MLG 72. We report the first N. caninum isolate from beef cattle in Argentina. EEA Balcarce Fil: Campero, Lucía María. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Dellarupe, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Schares, Gereon. Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Institute of Epidemiology; Alemania. Fil: Moré, Gastón. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Moore, Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2020-09-22T11:57:24Z 2020-09-22T11:57:24Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7936 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302197 2405-9390 https://doi.org/10.1016/j.vprsr.2020.100438 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology: Regional Studies and Reports 21 : 100438 (2020) |
| spellingShingle | Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites Campero, Lucía María Gual, Ignacio Dellarupe, Andrea Schares, Gereon Moré, Gastón Moore, Prando Dadin Venturini, María Cecilia Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title | Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title_full | Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title_fullStr | Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title_full_unstemmed | Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title_short | Isolation of Neospora caninum from a beef cattle fetus from Argentina: Immunopathological and molecular studies. |
| title_sort | isolation of neospora caninum from a beef cattle fetus from argentina immunopathological and molecular studies |
| topic | Ganado Bovino Neospora Caninum Virulencia Microsatélites Argentina Cattle Virulence Microsatellites |
| url | http://hdl.handle.net/20.500.12123/7936 https://www.sciencedirect.com/science/article/abs/pii/S2405939020302197 https://doi.org/10.1016/j.vprsr.2020.100438 |
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