Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance
The worldwide-distributed leaf peach curl disease is caused by the biotroph Taphrina deformans. To characterize the plant-fungus interaction, resistant and susceptible Prunus persica genotypes grown in the orchard were studied. Asymptomatic leaves were tested for fungal presence. In all resistant le...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/793 http://www.sciencedirect.com/science/article/pii/S0981942817302139?via%3Dihub https://doi.org/10.1016/j.plaphy.2017.06.022 |
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| author | Goldy, Camila Svetaz, Laura Andrea Bustamante, Claudia Anabel Allegrini, Marco Valentini, Gabriel Hugo Drincovich, María Fabiana Fernie, Alisdair R. Lara, Maria Valeria |
| author_browse | Allegrini, Marco Bustamante, Claudia Anabel Drincovich, María Fabiana Fernie, Alisdair R. Goldy, Camila Lara, Maria Valeria Svetaz, Laura Andrea Valentini, Gabriel Hugo |
| author_facet | Goldy, Camila Svetaz, Laura Andrea Bustamante, Claudia Anabel Allegrini, Marco Valentini, Gabriel Hugo Drincovich, María Fabiana Fernie, Alisdair R. Lara, Maria Valeria |
| author_sort | Goldy, Camila |
| collection | INTA Digital |
| description | The worldwide-distributed leaf peach curl disease is caused by the biotroph Taphrina deformans. To characterize the plant-fungus interaction, resistant and susceptible Prunus persica genotypes grown in the orchard were studied. Asymptomatic leaves were tested for fungal presence. In all resistant leaves analyzed the fungus was not detected. Conversely, leaves from the susceptible genotype were categorized according to the presence or absence of the pathogen.
Comparative metabolomic analysis disclosed the metabolite composition associated with resistant and susceptible interactions, and of compounds involved in fungal growth inhibition such as chlorogenic acid, whose in vitro antifungal activity was verified in this work.
Differential proteome studies revealed that chloroplasts are important site of plant defense responses against T. deformans. Members of the Bet-v1-like family protein differentially responded to the pathogen.
Extracellular pathogenesis-related proteins, evaluated by qRT-PCR, and an enone oxidoreductase are constitutively present in leaves of resistant trees and could be related to fungal resistance.
This study is a global view of the changes in the metabolome, proteome and transcripts related to plant defense in naturally infected leaves of susceptible plants during the asymptomatic stage. Additionally, it provides clues to the successful molecular mechanisms operating in resistant plants, which neither develop the disease nor harbor the pathogen. |
| format | Artículo |
| id | INTA793 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA7932019-06-06T17:06:37Z Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance Goldy, Camila Svetaz, Laura Andrea Bustamante, Claudia Anabel Allegrini, Marco Valentini, Gabriel Hugo Drincovich, María Fabiana Fernie, Alisdair R. Lara, Maria Valeria Prunus Persica Durazno Genotipos Taphrina deformans Resistencia a la Enfermedad Peaches Genotypes Disease Resistance The worldwide-distributed leaf peach curl disease is caused by the biotroph Taphrina deformans. To characterize the plant-fungus interaction, resistant and susceptible Prunus persica genotypes grown in the orchard were studied. Asymptomatic leaves were tested for fungal presence. In all resistant leaves analyzed the fungus was not detected. Conversely, leaves from the susceptible genotype were categorized according to the presence or absence of the pathogen. Comparative metabolomic analysis disclosed the metabolite composition associated with resistant and susceptible interactions, and of compounds involved in fungal growth inhibition such as chlorogenic acid, whose in vitro antifungal activity was verified in this work. Differential proteome studies revealed that chloroplasts are important site of plant defense responses against T. deformans. Members of the Bet-v1-like family protein differentially responded to the pathogen. Extracellular pathogenesis-related proteins, evaluated by qRT-PCR, and an enone oxidoreductase are constitutively present in leaves of resistant trees and could be related to fungal resistance. This study is a global view of the changes in the metabolome, proteome and transcripts related to plant defense in naturally infected leaves of susceptible plants during the asymptomatic stage. Additionally, it provides clues to the successful molecular mechanisms operating in resistant plants, which neither develop the disease nor harbor the pathogen. EEA San Pedro Fil: Goldy, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Svetaz, Laura Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Bustamante, Claudia A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Allegrini, Marco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Fernie, Alisdair R. Max-Planck-Institut fur Molekulare Pflanzenphysiologie; Alemania Fil: Lara, Maria Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2017-07-27T12:26:50Z 2017-07-27T12:26:50Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://hdl.handle.net/20.500.12123/793 http://www.sciencedirect.com/science/article/pii/S0981942817302139?via%3Dihub 0981-9428 https://doi.org/10.1016/j.plaphy.2017.06.022 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant physiology and biochemistry 118 : 245-255. (2017) |
| spellingShingle | Prunus Persica Durazno Genotipos Taphrina deformans Resistencia a la Enfermedad Peaches Genotypes Disease Resistance Goldy, Camila Svetaz, Laura Andrea Bustamante, Claudia Anabel Allegrini, Marco Valentini, Gabriel Hugo Drincovich, María Fabiana Fernie, Alisdair R. Lara, Maria Valeria Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title | Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title_full | Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title_fullStr | Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title_full_unstemmed | Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title_short | Comparative proteomic and metabolomic studies between Prunus persica genotypes resistant and susceptible to Taphrina deformans suggest a molecular basis of resistance |
| title_sort | comparative proteomic and metabolomic studies between prunus persica genotypes resistant and susceptible to taphrina deformans suggest a molecular basis of resistance |
| topic | Prunus Persica Durazno Genotipos Taphrina deformans Resistencia a la Enfermedad Peaches Genotypes Disease Resistance |
| url | http://hdl.handle.net/20.500.12123/793 http://www.sciencedirect.com/science/article/pii/S0981942817302139?via%3Dihub https://doi.org/10.1016/j.plaphy.2017.06.022 |
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