Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis
Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pa...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7921 https://www.nature.com/articles/s41598-020-70315-4 https://doi.org/10.1038/s41598-020-70315-4 |
| _version_ | 1855035943718748160 |
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| author | Fass, Monica Irina Rivarola, Maximo Lisandro Ehrenbolger, Guillermo Federico Maringolo, Carla Andrea Montecchia, Juan Francisco Quiroz, Facundo Jose García‑García, Francisco Dopazo Blázquez, Joaquín Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_browse | Dopazo Blázquez, Joaquín Ehrenbolger, Guillermo Federico Fass, Monica Irina García‑García, Francisco Heinz, Ruth Amelia Hopp, Horacio Esteban Lia, Veronica Viviana Maringolo, Carla Andrea Montecchia, Juan Francisco Paniego, Norma Beatriz Quiroz, Facundo Jose Rivarola, Maximo Lisandro |
| author_facet | Fass, Monica Irina Rivarola, Maximo Lisandro Ehrenbolger, Guillermo Federico Maringolo, Carla Andrea Montecchia, Juan Francisco Quiroz, Facundo Jose García‑García, Francisco Dopazo Blázquez, Joaquín Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana |
| author_sort | Fass, Monica Irina |
| collection | INTA Digital |
| description | Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7921 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA79212020-09-17T17:05:34Z Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis Fass, Monica Irina Rivarola, Maximo Lisandro Ehrenbolger, Guillermo Federico Maringolo, Carla Andrea Montecchia, Juan Francisco Quiroz, Facundo Jose García‑García, Francisco Dopazo Blázquez, Joaquín Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Helianthus annuus Sclerotinia sclerotiorum Expresión Génica Respuesta Inmunológica Mecanismo de Defensa Secuencia de ARN Gene Expression Immune Response Defense Mechanisms RNA Sequence Sclerotinia head rot (SHR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating sunflower crop diseases. Despite its worldwide occurrence, the genetic determinants of plant resistance are still largely unknown. Here, we investigated the Sclerotinia-sunflower pathosystem by analysing temporal changes in gene expression in one susceptible and two tolerant inbred lines (IL) inoculated with the pathogen under field conditions. Differential expression analysis showed little overlapping among ILs, suggesting genotype-specific control of cell defense responses possibly related to differences in disease resistance strategies. Functional enrichment assessments yielded a similar pattern. However, all three ILs altered the expression of genes involved in the cellular redox state and cell wall remodeling, in agreement with current knowledge about the initiation of plant immune responses. Remarkably, the over-representation of long non-coding RNAs (lncRNA) was another common feature among ILs. Our findings highlight the diversity of transcriptional responses to SHR within sunflower breeding lines and provide evidence of lncRNAs playing a significant role at early stages of defense. Instituto de Biotecnología Fil: Fass, Mónica Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ehrenbolger, Guillermo Federico. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maringolo, Carla Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: Montecchia, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quiroz, Facundo Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Patología Vegetal; Argentina Fil: García‑García, Francisco. Principe Felipe Research Center. Bioinfomatics and Biostatistics Unit; España Fil: Dopazo Blázquez, Joaquín. Fundación Progreso y Salud (FPS). Clinical Bioinformatics Area; España Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lia, Veronica Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-17T16:59:34Z 2020-09-17T16:59:34Z 2020-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7921 https://www.nature.com/articles/s41598-020-70315-4 2045-2322 https://doi.org/10.1038/s41598-020-70315-4 eng info:eu-repograntAgreement/INTA/PNBIO-1131022/AR./Genómica funcional y biología de sistemas. info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Nature Scientific Reports 10 : 13347 (Agosto 2020) |
| spellingShingle | Helianthus annuus Sclerotinia sclerotiorum Expresión Génica Respuesta Inmunológica Mecanismo de Defensa Secuencia de ARN Gene Expression Immune Response Defense Mechanisms RNA Sequence Fass, Monica Irina Rivarola, Maximo Lisandro Ehrenbolger, Guillermo Federico Maringolo, Carla Andrea Montecchia, Juan Francisco Quiroz, Facundo Jose García‑García, Francisco Dopazo Blázquez, Joaquín Hopp, Horacio Esteban Heinz, Ruth Amelia Paniego, Norma Beatriz Lia, Veronica Viviana Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title | Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title_full | Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title_fullStr | Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title_full_unstemmed | Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title_short | Exploring sunflower responses to Sclerotinia head rot at early stages of infection using RNA‑seq analysis |
| title_sort | exploring sunflower responses to sclerotinia head rot at early stages of infection using rna seq analysis |
| topic | Helianthus annuus Sclerotinia sclerotiorum Expresión Génica Respuesta Inmunológica Mecanismo de Defensa Secuencia de ARN Gene Expression Immune Response Defense Mechanisms RNA Sequence |
| url | http://hdl.handle.net/20.500.12123/7921 https://www.nature.com/articles/s41598-020-70315-4 https://doi.org/10.1038/s41598-020-70315-4 |
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