Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento
Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed p...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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CIAT
2020
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| Online Access: | http://www.tropicalgrasslands.info/index.php/tgft/article/view/348 http://hdl.handle.net/20.500.12123/7917 https://doi.org/10.17138/tgft(5)117-128 |
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| author | Armando, Lorena Tomas, Maria Andrea Garayalde, Antonio Francisco Carrera, Alicia Delia |
| author_browse | Armando, Lorena Carrera, Alicia Delia Garayalde, Antonio Francisco Tomas, Maria Andrea |
| author_facet | Armando, Lorena Tomas, Maria Andrea Garayalde, Antonio Francisco Carrera, Alicia Delia |
| author_sort | Armando, Lorena |
| collection | INTA Digital |
| description | Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed production and further survival of the progeny. We evaluated self- and open-pollinated progenies from different accessions by measuring the seed production of the parents and their germination performance, germination rate and seedling survival. Parents and progeny were also fingerprinted with Simple Sequence Repeats (SSR). Progeny produced through open-pollination resulted in significantly more filled seeds and superior seedling survival than self-pollination. These results indicate that accessions studied here rely heavily on cross-pollination, whereas the contribution of self-pollinated offspring to the population is likely to be low. SSR profiles showed that, on average, 85% of the progeny (arising from cross-pollination) possessed paternal specific markers and 100% of them were genetically different from the maternal genotype. All plants examined had 4x = 36 chromosomes. Overall, our findings indicate that var. makarikariense is able to generate highly polymorphic progeny through segregation and recombination. This study provides reference information for the formulation of appropriate strategies for pasture germplasm management, conservation and development of breeding programs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7917 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | CIAT |
| publisherStr | CIAT |
| record_format | dspace |
| spelling | INTA79172020-09-17T13:41:55Z Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento Armando, Lorena Tomas, Maria Andrea Garayalde, Antonio Francisco Carrera, Alicia Delia Panicum Coloratum Fitomejoramiento Variación Genética Polinización Poliploidia Plant Breeding Genetic Variation Pollination Polyploidy Panicum coloratum var. makarikariense, a perennial grass native to Africa, is adapted to a wide range of soil and climatic conditions with potential to be used as forage in tropical and semi-arid regions around the world. Our objective was to understand how the pollination mode affects viable seed production and further survival of the progeny. We evaluated self- and open-pollinated progenies from different accessions by measuring the seed production of the parents and their germination performance, germination rate and seedling survival. Parents and progeny were also fingerprinted with Simple Sequence Repeats (SSR). Progeny produced through open-pollination resulted in significantly more filled seeds and superior seedling survival than self-pollination. These results indicate that accessions studied here rely heavily on cross-pollination, whereas the contribution of self-pollinated offspring to the population is likely to be low. SSR profiles showed that, on average, 85% of the progeny (arising from cross-pollination) possessed paternal specific markers and 100% of them were genetically different from the maternal genotype. All plants examined had 4x = 36 chromosomes. Overall, our findings indicate that var. makarikariense is able to generate highly polymorphic progeny through segregation and recombination. This study provides reference information for the formulation of appropriate strategies for pasture germplasm management, conservation and development of breeding programs. Panicum coloratum var. makarikariense es una gramínea perenne nativa de África. Se adapta a un amplio rango de ambientes y posee uso potencial como forraje en distintas regiones tropicales y semiáridas del mundo. El estudio tuvo como objetivo evaluar el efecto del modo de polinización sobre la producción de semilla viable y la supervivencia de la progenie. Se evaluaron progenies de autopolinización y de polinización cruzada en diferentes accesiones midiendo la producción de semillas, germinación, tasa de germinación y supervivencia de plántulas, y se obtuvieron perfiles moleculares con Secuencias Simples Repetidas (SSR). La progenie obtenida mediante polinización cruzada mostró significativamente mayor producción de semillas llenas y supervivencia de plántulas que la de autopolinización. Esto indica que las accesiones evaluadas dependen en gran medida de la alogamia y que la contribución de la descendencia por autofertilización a la población sería escasa. Los perfiles moleculares SSR mostraron que, en promedio, 85% de la progenie (obtenida a partir de polinización cruzada) presentó marcadores específicos paternos y 100% de ella difirió del genotipo materno. Todas las plantas examinadas presentaron 4x = 36 cromosomas. En conjunto, los resultados indican que la var. makarikariense puede generar progenie altamente polimórfica a través de la segregación y recombinación. Este estudio provee información útil para el diseño de estrategias de conservación, manejo del germoplasma y programas de mejoramiento. EEA Rafaela Fil: Armando, Lorena Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina Fil: Tomas, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Garayalde, Antonio Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Matemática; Argentina Fil: Carrera, Alicia Delia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina 2020-09-17T13:30:07Z 2020-09-17T13:30:07Z 2017-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://www.tropicalgrasslands.info/index.php/tgft/article/view/348 http://hdl.handle.net/20.500.12123/7917 2346-3775 https://doi.org/10.17138/tgft(5)117-128 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf CIAT Tropical grasslands-Forrajes tropicales 5 (3) : 117–128. (2017) |
| spellingShingle | Panicum Coloratum Fitomejoramiento Variación Genética Polinización Poliploidia Plant Breeding Genetic Variation Pollination Polyploidy Armando, Lorena Tomas, Maria Andrea Garayalde, Antonio Francisco Carrera, Alicia Delia Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title | Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title_full | Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title_fullStr | Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title_full_unstemmed | Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title_short | Effect of pollination mode on progeny of Panicum coloratum var. makarikariense: Implications for conservation and breeding = Efecto del modo de polinización sobre la progenie de Panicum coloratum var. makarikariense: Implicaciones para conservación y fitomejoramiento |
| title_sort | effect of pollination mode on progeny of panicum coloratum var makarikariense implications for conservation and breeding efecto del modo de polinizacion sobre la progenie de panicum coloratum var makarikariense implicaciones para conservacion y fitomejoramiento |
| topic | Panicum Coloratum Fitomejoramiento Variación Genética Polinización Poliploidia Plant Breeding Genetic Variation Pollination Polyploidy |
| url | http://www.tropicalgrasslands.info/index.php/tgft/article/view/348 http://hdl.handle.net/20.500.12123/7917 https://doi.org/10.17138/tgft(5)117-128 |
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