The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far?
Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to d...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Crimson Publishers
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/7913 https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php http://doi.org/10.31031/MCDA.2020.07.000656 |
| _version_ | 1855484098138603520 |
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| author | Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
| author_browse | Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
| author_facet | Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela |
| author_sort | Darqui, Flavia Soledad |
| collection | INTA Digital |
| description | Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies. |
| format | Artículo |
| id | INTA7913 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Crimson Publishers |
| publisherStr | Crimson Publishers |
| record_format | dspace |
| spelling | INTA79132020-09-16T17:52:28Z The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa Specific mutations in the conserved domains of the acetolactate synthase (ALS) gene conduct to different key amino acid substitutions that can confer herbicide resistance in different plant species. This outcome has been widely exploited to produce herbicide-resistant agronomic crops as well as to direct many genome editing studies. Therefore, the ALS gene has become a model sequence target to improve our technological skills for more precise CRISPR/Cas nucleotide base substitution in plants, which is essential for modulation/modification of gene function as opposed to the more general gene knock out obtained by indels in conventional genome editing studies. This review summarizes the main knowledge and experiences attained from the use of the ALS gene as a target in CRISPR/Cas studies. Instituto de Biotecnología Fil: Darqui, Flavia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular; Argentina Fil: Lopez Bilbao, Marisa Gisela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-16T17:47:50Z 2020-09-16T17:47:50Z 2020-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7913 https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php 2637-7659 http://doi.org/10.31031/MCDA.2020.07.000656 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Crimson Publishers Modern Concepts & Developments in Agronomy 7 (2) : 700-711 (Agosto 2020) |
| spellingShingle | Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa Darqui, Flavia Soledad Hopp, Horacio Esteban Lopez Bilbao, Marisa Gisela The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title | The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title_full | The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title_fullStr | The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title_full_unstemmed | The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title_short | The ALS gene as genetic target in CRISPR/ cas approaches: what have we learned so far? |
| title_sort | als gene as genetic target in crispr cas approaches what have we learned so far |
| topic | Mutagenesis Amino Acids Resistance to Herbicides Mutagénesis Aminoácidos Resistencia a los Herbicidas Genes Acetolactate Synthase Acetolactato Sintasa |
| url | http://hdl.handle.net/20.500.12123/7913 https://crimsonpublishers.com/mcda/fulltext/MCDA.000656.php http://doi.org/10.31031/MCDA.2020.07.000656 |
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