Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences
Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Ja...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7894 https://link.springer.com/article/10.1007/s00436-019-06591-5 https://doi.org/10.1007/s00436-019-06591-5 |
| _version_ | 1855035939171074048 |
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| author | Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_browse | Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Liendo, María Clara Muntaabski, Irina Palacio, María Alejandra Russo, Romina Maria Scannapieco, Alejandra Carla |
| author_facet | Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
| author_sort | Muntaabski, Irina |
| collection | INTA Digital |
| description | Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7894 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA78942020-09-15T11:52:57Z Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla Genetic Variation Genetic Markers Variación Genética Marcadores Genéticos Apis mellifera Varroa destructor Argentina Haplotypes Heteroplasmy Haplotipos Heteroplasmia Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor. Instituto de Genética Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Palacio, María Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-15T11:35:37Z 2020-09-15T11:35:37Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7894 https://link.springer.com/article/10.1007/s00436-019-06591-5 1432-1955 https://doi.org/10.1007/s00436-019-06591-5 eng info:eu-repograntAgreement/INTA/PNAPI-1112042/AR./Estrategias multidisciplinarias para mitigar el efecto del nuevo contexto ambiental y productivo sobre la colmena. info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 119 (2) : 411-421 (Febrero 2020) |
| spellingShingle | Genetic Variation Genetic Markers Variación Genética Marcadores Genéticos Apis mellifera Varroa destructor Argentina Haplotypes Heteroplasmy Haplotipos Heteroplasmia Muntaabski, Irina Russo, Romina Maria Liendo, María Clara Palacio, María Alejandra Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title | Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title_full | Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title_fullStr | Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title_full_unstemmed | Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title_short | Genetic variation and heteroplasmy of Varroa destructor inferred from ND4 mtDNA sequences |
| title_sort | genetic variation and heteroplasmy of varroa destructor inferred from nd4 mtdna sequences |
| topic | Genetic Variation Genetic Markers Variación Genética Marcadores Genéticos Apis mellifera Varroa destructor Argentina Haplotypes Heteroplasmy Haplotipos Heteroplasmia |
| url | http://hdl.handle.net/20.500.12123/7894 https://link.springer.com/article/10.1007/s00436-019-06591-5 https://doi.org/10.1007/s00436-019-06591-5 |
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