A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America
The aim of this work was to report the detection of a putative novel Ehrlichia strain associated with the tick Amblyomma triste. Free-living adult ticks determined as A. triste were collected by drag-sampling in Argentina and Uruguay. Molecular detection of Ehrlichia agents was performed targeting t...
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Taylor & Francis
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7870 https://doi.org/10.1080/20477724.2020.1795579 |
| _version_ | 1855035935039684608 |
|---|---|
| author | Cicuttin, Gabriel L. De Salvo, María Nazarena Díaz Pérez, Paula Silva, Darío Félix, María Laura Venzal, José Manuel Nava Santiago |
| author_browse | Cicuttin, Gabriel L. De Salvo, María Nazarena Díaz Pérez, Paula Félix, María Laura Nava Santiago Silva, Darío Venzal, José Manuel |
| author_facet | Cicuttin, Gabriel L. De Salvo, María Nazarena Díaz Pérez, Paula Silva, Darío Félix, María Laura Venzal, José Manuel Nava Santiago |
| author_sort | Cicuttin, Gabriel L. |
| collection | INTA Digital |
| description | The aim of this work was to report the detection of a putative novel Ehrlichia strain associated with the tick Amblyomma triste. Free-living adult ticks determined as A. triste were collected by drag-sampling in Argentina and Uruguay. Molecular detection of Ehrlichia agents was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. In total, 164 adults of A. triste (38 from INTA E.E.A Delta del Paraná in Argentina and 126 from Toledo Chico in Uruguay) were analyzed. One tick (0.6%) collected in INTA E.E.A. Delta del Paraná (Argentina) was positive. The phylogenetic analyses show that the Ehrlichia strain found in this study (named Ehrlichia sp. strain Delta) represents an independent lineage within the genus Ehrlichia, close to E. chaffeensis and E. muris. This is also the first report of an Ehrlichia agent infecting the tick A. triste. The medical and veterinary significance of Ehrlichia sp. strain Delta remains to be demonstrated. However, it is important to mention that adults of A. triste are aggressive to humans and domestic mammals. Therefore, the potential role of A. triste in the transmission of Ehrlichia agents to humans or domestic animals across its distributional range should be highlighted, even more considering that Ehrlichia sp. strain Delta is phylogenetically related to the zoonotic E. chaffeensis, which is recognized as pathogenic to both humans and animals. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7870 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Taylor & Francis |
| publisherStr | Taylor & Francis |
| record_format | dspace |
| spelling | INTA78702020-09-10T20:09:14Z A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America Cicuttin, Gabriel L. De Salvo, María Nazarena Díaz Pérez, Paula Silva, Darío Félix, María Laura Venzal, José Manuel Nava Santiago Ehrlichia Metastigmata Amblyomma América del Sur South America Ticks Garrapatas The aim of this work was to report the detection of a putative novel Ehrlichia strain associated with the tick Amblyomma triste. Free-living adult ticks determined as A. triste were collected by drag-sampling in Argentina and Uruguay. Molecular detection of Ehrlichia agents was performed targeting three different loci: 16S rRNA gene, dsb gene and a fragment of groESL heat shock operon. In total, 164 adults of A. triste (38 from INTA E.E.A Delta del Paraná in Argentina and 126 from Toledo Chico in Uruguay) were analyzed. One tick (0.6%) collected in INTA E.E.A. Delta del Paraná (Argentina) was positive. The phylogenetic analyses show that the Ehrlichia strain found in this study (named Ehrlichia sp. strain Delta) represents an independent lineage within the genus Ehrlichia, close to E. chaffeensis and E. muris. This is also the first report of an Ehrlichia agent infecting the tick A. triste. The medical and veterinary significance of Ehrlichia sp. strain Delta remains to be demonstrated. However, it is important to mention that adults of A. triste are aggressive to humans and domestic mammals. Therefore, the potential role of A. triste in the transmission of Ehrlichia agents to humans or domestic animals across its distributional range should be highlighted, even more considering that Ehrlichia sp. strain Delta is phylogenetically related to the zoonotic E. chaffeensis, which is recognized as pathogenic to both humans and animals. EEA Rafaela Fil: Cicuttin, Gabriel L. Instituto de Zoonosis Luis Pasteur; Argentina Fil: De Salvo, María Nazarena. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Díaz Pérez, Paula. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Silva, Darío. Instituto de Zoonosis Luis Pasteur; Argentina Fil: Félix, María Laura. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina 2020-09-10T17:36:52Z 2020-09-10T17:36:52Z 2020 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7870 Gabriel L. Cicuttin , María N. De Salvo , Paula Díaz Pérez , Darío Silva , María L. Félix , José M. Venzal & Santiago Nava (2020): A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America, Pathogens and Global Health, DOI: 10.1080/20477724.2020.1795579 2047-7724 2047-7732 (Online) https://doi.org/10.1080/20477724.2020.1795579 eng info:eu-repo/semantics/restrictedAccess application/pdf Taylor & Francis Pathogens and global health 114 (6) : 318-322. (2020) |
| spellingShingle | Ehrlichia Metastigmata Amblyomma América del Sur South America Ticks Garrapatas Cicuttin, Gabriel L. De Salvo, María Nazarena Díaz Pérez, Paula Silva, Darío Félix, María Laura Venzal, José Manuel Nava Santiago A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title | A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title_full | A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title_fullStr | A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title_full_unstemmed | A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title_short | A novel Ehrlichia strain (Rickettsiales: Anaplasmataceae) detected in Amblyomma triste (Acari: Ixodidae), a tick species of public health importance in the Southern Cone of America |
| title_sort | novel ehrlichia strain rickettsiales anaplasmataceae detected in amblyomma triste acari ixodidae a tick species of public health importance in the southern cone of america |
| topic | Ehrlichia Metastigmata Amblyomma América del Sur South America Ticks Garrapatas |
| url | http://hdl.handle.net/20.500.12123/7870 https://doi.org/10.1080/20477724.2020.1795579 |
| work_keys_str_mv | AT cicuttingabriell anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT desalvomarianazarena anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT diazperezpaula anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT silvadario anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT felixmarialaura anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT venzaljosemanuel anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT navasantiago anovelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT cicuttingabriell novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT desalvomarianazarena novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT diazperezpaula novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT silvadario novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT felixmarialaura novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT venzaljosemanuel novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica AT navasantiago novelehrlichiastrainrickettsialesanaplasmataceaedetectedinamblyommatristeacariixodidaeatickspeciesofpublichealthimportanceinthesouthernconeofamerica |