Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae)
Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increase...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Entomological Society of America
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7860 https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/5858931 https://doi.org/10.1093/jme/tjaa099 |
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| author | Rolandi, Carmen Roca-Acevedo, Gonzalo Schilman, Pablo Ernesto Germano, Monica Daniela |
| author_browse | Germano, Monica Daniela Roca-Acevedo, Gonzalo Rolandi, Carmen Schilman, Pablo Ernesto |
| author_facet | Rolandi, Carmen Roca-Acevedo, Gonzalo Schilman, Pablo Ernesto Germano, Monica Daniela |
| author_sort | Rolandi, Carmen |
| collection | INTA Digital |
| description | Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7860 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Entomological Society of America |
| publisherStr | Entomological Society of America |
| record_format | dspace |
| spelling | INTA78602020-09-10T11:35:33Z Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) Rolandi, Carmen Roca-Acevedo, Gonzalo Schilman, Pablo Ernesto Germano, Monica Daniela Triatoma Infestans Enfermedad de Chagas Insecta Resistencia a los Insecticidas Chagas' disease Insecticide Resistance Triatoma infestans (Klug, 1834), the main vector of Chagas disease in Latin America, is regularly controlled by spraying the pyrethroid deltamethrin, to which some populations have developed resistance. The three main mechanisms of resistance are 1) metabolic resistance by overexpression or increased activity of detoxifying enzymes, 2) target site mutations, and 3) cuticle thickening/modification. We use open-flow respirometry to measure real-time H2O loss rate (V˙ H2O) and CO2 production rate (V˙ CO2), on nymphs from susceptible and resistant populations before and after exposure to the insecticide to understand the underlying mechanisms of resistance in live insects. Lack of differences in V˙ H2O between populations suggested that cuticular thickness/composition is not acting as a relevant resistance mechanism. Similarly, there was no difference in resting V˙ CO2, suggesting a trade-off between resistance mechanisms and other physiological processes. The increment in V˙ CO2 after application of deltamethrin was similar in both populations, which suggested that while enhanced enzymatic detoxification may play a role in resistance expression in this population, the main mechanism involved should be a passive one such as target site mutations. Open-flow respirometry provided useful evidence for evaluating the mechanisms involved in deltamethrin resistance. Using this technique could improve efficiency of scientific research in the area of insecticide resistance management, leading to a faster decision making and hence improved control results. Estación Experimental Agropecuaria Bariloche Fil: Rolandi, Carmen. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina Fil: Roca-Acevedo, Gonzalo. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones de Plagas e Insecticidas; Argentina Fil: Schilman, Pablo Ernesto. Universidad de Buenos Aires. Consejo Nacional de Investigaciones Científicas y Técnicas. Laboratorio de Ecofisiología de Insectos; Argentina Fil: Germano, Monica Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Campo Forestal General San Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina 2020-09-10T11:27:50Z 2020-09-10T11:27:50Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7860 https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/5858931 1938-2928 0022-2585 https://doi.org/10.1093/jme/tjaa099 eng info:eu-repo/semantics/restrictedAccess application/pdf Entomological Society of America Journal of Medical Entomology : art: tjaa099 (Junio 2020) |
| spellingShingle | Triatoma Infestans Enfermedad de Chagas Insecta Resistencia a los Insecticidas Chagas' disease Insecticide Resistance Rolandi, Carmen Roca-Acevedo, Gonzalo Schilman, Pablo Ernesto Germano, Monica Daniela Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title | Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title_full | Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title_fullStr | Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title_full_unstemmed | Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title_short | Aerobic Metabolism Alterations as an Evidence of Underlying Deltamethrin Resistance Mechanisms in Triatoma infestans (Hemiptera: Reduviidae) |
| title_sort | aerobic metabolism alterations as an evidence of underlying deltamethrin resistance mechanisms in triatoma infestans hemiptera reduviidae |
| topic | Triatoma Infestans Enfermedad de Chagas Insecta Resistencia a los Insecticidas Chagas' disease Insecticide Resistance |
| url | http://hdl.handle.net/20.500.12123/7860 https://academic.oup.com/jme/advance-article-abstract/doi/10.1093/jme/tjaa099/5858931 https://doi.org/10.1093/jme/tjaa099 |
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