Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively
Entomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and pathogenesis. Here, we performed an Azan trichrome stain for light microscopic vi...
| Autores principales: | , , |
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| Formato: | info:eu-repo/semantics/article |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/785 http://www.scielo.org.ar/pdf/ram/v48n4/v48n4a17.pdf http://ac.els-cdn.com/S0325754116300505/1-s2.0-S0325754116300505-main.pdf?_tid=23199db6-720f-11e7-8d75-00000aab0f27&acdnat=1501079679_d9fb0fa263a66265937d595ac24e3e5a http://dx.doi.org/10.1016/j.ram.2016.04.008 |
| _version_ | 1855034702858027008 |
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| author | Arneodo Larochette, Joel Demian Taibo, Catalina Beatriz Sciocco, Alicia Ines |
| author_browse | Arneodo Larochette, Joel Demian Sciocco, Alicia Ines Taibo, Catalina Beatriz |
| author_facet | Arneodo Larochette, Joel Demian Taibo, Catalina Beatriz Sciocco, Alicia Ines |
| author_sort | Arneodo Larochette, Joel Demian |
| collection | INTA Digital |
| description | Entomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and pathogenesis. Here, we performed an Azan trichrome stain for light microscopic visualization of pathogens in the larval tissues of two Neotropical lepidopterans.
Epinotia aporema larvae exhibiting symptoms of microsporidiosis were fortuitously detected in a rearing facility (IMyZA-INTA, Argentina), while Helicoverpa gelotopoeon specimens were experimentally infected with an Argentinean isolate of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Caterpillars were fixed in Duboscq-Brasil and paraffin embedded. Microtome sections (5 μm-thick) were deparaffinized and stained2 for observation under a Nikon Eclipse 80i microscope equipped with a Nikon DS-U3 digital camera. |
| format | info:eu-repo/semantics/article |
| id | INTA785 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA7852018-01-11T15:10:34Z Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively Arneodo Larochette, Joel Demian Taibo, Catalina Beatriz Sciocco, Alicia Ines Plagas de plantas Microscopia Epinotia aporema Helicoverpa Pest of Plants Microscopy Entomopatógenos Entomopathogens help regulate natural insect populations, and their use in pest management is the subject of active research. Classical and molecular techniques are currently available to elucidate disease etiology and pathogenesis. Here, we performed an Azan trichrome stain for light microscopic visualization of pathogens in the larval tissues of two Neotropical lepidopterans. Epinotia aporema larvae exhibiting symptoms of microsporidiosis were fortuitously detected in a rearing facility (IMyZA-INTA, Argentina), while Helicoverpa gelotopoeon specimens were experimentally infected with an Argentinean isolate of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Caterpillars were fixed in Duboscq-Brasil and paraffin embedded. Microtome sections (5 μm-thick) were deparaffinized and stained2 for observation under a Nikon Eclipse 80i microscope equipped with a Nikon DS-U3 digital camera. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Servicio de Microscopía; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina 2017-07-26T15:31:20Z 2017-07-26T15:31:20Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/785 http://www.scielo.org.ar/pdf/ram/v48n4/v48n4a17.pdf http://ac.els-cdn.com/S0325754116300505/1-s2.0-S0325754116300505-main.pdf?_tid=23199db6-720f-11e7-8d75-00000aab0f27&acdnat=1501079679_d9fb0fa263a66265937d595ac24e3e5a 1851-7617 http://dx.doi.org/10.1016/j.ram.2016.04.008 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Revista Argentina de Microbiología 48 (4) : 358-359 |
| spellingShingle | Plagas de plantas Microscopia Epinotia aporema Helicoverpa Pest of Plants Microscopy Entomopatógenos Arneodo Larochette, Joel Demian Taibo, Catalina Beatriz Sciocco, Alicia Ines Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title | Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title_full | Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title_fullStr | Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title_full_unstemmed | Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title_short | Light microscopy of microsporidian spores and baculovirus occlusion bodies in Epinotia aporema and Helicoverpa gelotopoeon larval tissues, respectively |
| title_sort | light microscopy of microsporidian spores and baculovirus occlusion bodies in epinotia aporema and helicoverpa gelotopoeon larval tissues respectively |
| topic | Plagas de plantas Microscopia Epinotia aporema Helicoverpa Pest of Plants Microscopy Entomopatógenos |
| url | http://hdl.handle.net/20.500.12123/785 http://www.scielo.org.ar/pdf/ram/v48n4/v48n4a17.pdf http://ac.els-cdn.com/S0325754116300505/1-s2.0-S0325754116300505-main.pdf?_tid=23199db6-720f-11e7-8d75-00000aab0f27&acdnat=1501079679_d9fb0fa263a66265937d595ac24e3e5a http://dx.doi.org/10.1016/j.ram.2016.04.008 |
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