Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils
Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can rapidly disperse across the planet by multiple ways. Thus, microbial plasmids,...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7848 https://www.sciencedirect.com/science/article/pii/S2213716520300163 https://doi.org/10.1016/j.jgar.2020.01.015 |
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| author | Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Soto, Gabriela Cynthia Berini, Carolina Andrea Jozefkowicz, Cintia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Berini, Carolina Andrea Brambilla, Silvina Maricel Frare, Romina Alejandra Jozefkowicz, Cintia Soto, Gabriela Cynthia Stritzler, Margarita |
| author_facet | Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Soto, Gabriela Cynthia Berini, Carolina Andrea Jozefkowicz, Cintia Ayub, Nicolás Daniel |
| author_sort | Brambilla, Silvina Maricel |
| collection | INTA Digital |
| description | Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can rapidly disperse across the planet by multiple ways. Thus, microbial plasmids, including synthetic replicons, containing antibiotic resistance genes are a serious risk to public health. In this short communication, we explored the presence of synthetic elements in alfalfa symbionts (Ensifer meliloti strains) from agricultural soils.
Methods: A total of 148 E. meliloti isolates from alfalfa plants growing under field conditions were collected from January 2015 to June 2019. Antimicrobial susceptibility testing was performed under laboratory conditions. We identified five kanamycin-resistant E. meliloti strains (named K1-K5). Whole genome sequencing analysis and conjugations were used to identify and study the plasmids of K strains.
Results: We found that the genomes of K strains contain ampicillin, kanamycin and tetracycline resistance genes, the reporter gene lacZ from Escherichia coli and multiple cloning sites. These sequences were found within <58-kb plasmids related to the self-transmissible IncP plasmid RP4 from human pathogen Pseudomonas aeruginosa. Conjugation experiments confirmed the ability of K strains to transfer antibiotic resistance via conjugation to the Pseudomonas background.
Conclusion: In addition to the traditional analysis of plant growth-promoting factors, the commercial deregulation of putative natural inoculants should also include genomic studies to ensure a reasonable balance between innovation and caution. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7848 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA78482020-09-08T16:48:03Z Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Soto, Gabriela Cynthia Berini, Carolina Andrea Jozefkowicz, Cintia Ayub, Nicolás Daniel Genetically Modified Organisms Genetically Modified Microorganisms Resistance to Antibiotics Beta Galactosidase Organismos Modificados Genéticamente Microorganismos Modificados Genéticamente Resistencia a los Antibióticos Objectives: Unlike higher organisms such as domestic animals and cultivated plants, which display a robust reproductive isolation and limited dispersal ability, microbes exhibit an extremely promiscuous gene flow and can rapidly disperse across the planet by multiple ways. Thus, microbial plasmids, including synthetic replicons, containing antibiotic resistance genes are a serious risk to public health. In this short communication, we explored the presence of synthetic elements in alfalfa symbionts (Ensifer meliloti strains) from agricultural soils. Methods: A total of 148 E. meliloti isolates from alfalfa plants growing under field conditions were collected from January 2015 to June 2019. Antimicrobial susceptibility testing was performed under laboratory conditions. We identified five kanamycin-resistant E. meliloti strains (named K1-K5). Whole genome sequencing analysis and conjugations were used to identify and study the plasmids of K strains. Results: We found that the genomes of K strains contain ampicillin, kanamycin and tetracycline resistance genes, the reporter gene lacZ from Escherichia coli and multiple cloning sites. These sequences were found within <58-kb plasmids related to the self-transmissible IncP plasmid RP4 from human pathogen Pseudomonas aeruginosa. Conjugation experiments confirmed the ability of K strains to transfer antibiotic resistance via conjugation to the Pseudomonas background. Conclusion: In addition to the traditional analysis of plant growth-promoting factors, the commercial deregulation of putative natural inoculants should also include genomic studies to ensure a reasonable balance between innovation and caution. Instituto de Genética Fil: Brambilla, Silvina Maricel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berini, Carolina Andrea. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jozefkowicz, Cintia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-08T16:40:06Z 2020-09-08T16:40:06Z 2020-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7848 https://www.sciencedirect.com/science/article/pii/S2213716520300163 2213-7173 https://doi.org/10.1016/j.jgar.2020.01.015 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of Global Antimicrobial Resistance 22 : 113-116 (Septiembre 2020) |
| spellingShingle | Genetically Modified Organisms Genetically Modified Microorganisms Resistance to Antibiotics Beta Galactosidase Organismos Modificados Genéticamente Microorganismos Modificados Genéticamente Resistencia a los Antibióticos Brambilla, Silvina Maricel Frare, Romina Alejandra Stritzler, Margarita Soto, Gabriela Cynthia Berini, Carolina Andrea Jozefkowicz, Cintia Ayub, Nicolás Daniel Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title | Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title_full | Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title_fullStr | Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title_full_unstemmed | Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title_short | Synthetic multi-antibiotic resistant plasmids in plant-associated bacteria from agricultural soils |
| title_sort | synthetic multi antibiotic resistant plasmids in plant associated bacteria from agricultural soils |
| topic | Genetically Modified Organisms Genetically Modified Microorganisms Resistance to Antibiotics Beta Galactosidase Organismos Modificados Genéticamente Microorganismos Modificados Genéticamente Resistencia a los Antibióticos |
| url | http://hdl.handle.net/20.500.12123/7848 https://www.sciencedirect.com/science/article/pii/S2213716520300163 https://doi.org/10.1016/j.jgar.2020.01.015 |
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