Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina

The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens...

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Main Authors: Flores, Fernando Sebastián, Saracho Bottero, Maria Noelia, Sebastian, Patrick Stephan, Venzal, José Manuel, Mangold, Atilio Jose, Nava, Santiago
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2020
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Online Access:http://hdl.handle.net/20.500.12123/7827
https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304155
https://doi.org/10.1016/j.ttbdis.2020.101546
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author Flores, Fernando Sebastián
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
author_browse Flores, Fernando Sebastián
Mangold, Atilio Jose
Nava, Santiago
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
author_facet Flores, Fernando Sebastián
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
author_sort Flores, Fernando Sebastián
collection INTA Digital
description The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA78272021-09-13T14:28:34Z Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina Flores, Fernando Sebastián Saracho Bottero, Maria Noelia Sebastian, Patrick Stephan Venzal, José Manuel Mangold, Atilio Jose Nava, Santiago Ixodes Ixodes ricinus Identificación Argentina Identification Garrapatas Borrelia Ticks The aim of this work was to evaluate the presence of Borrelia infection in Ixodes sp. cf. Ixodes affinis ticks from Argentina. Specimens of Ixodes sp. cf. I. affinis were collected on vegetation and birds in five locations belonging the most humid part of the Chaco Biogeographic Province. Specimens were tested for Borrelia infection by nested-PCR targeting the flaB gene and the rrfA-rrlB intergenic spacer region (IGS), sequenced and phylogenetically analyzed. A total of 48 Ixodes sp. cf. I. affinis (12 questing adults from vegetation and 20 nymphs and 16 larvae on nine bird species: Arremon flavirostris, Basileuterus culicivorus, Campylorhamphus trochilirostris, Myiothlypis leucoblephara, Tachyphonus rufus, Thlypopsis sordida, Turdus amaurochalinus, Turdus rufiventris and Troglodytes aedon) were collected. Twelve adults, 14 nymphs and 11 larvae (3 individually and 8 in 3 pools) were analyzed. Partial sequences were detected in 6 adults, 11 nymphs and 4 larvae (2 individual and 2 pools). Phylogenetically, the Borrelia found in Ixodes sp. cf. I. affinis belongs to the B. burgdorferi sensu lato (s. l.) complex. The partial sequences obtained from the borrelian gene flaB and IGS were associated to two groups formed by sequences previously detected in Ixodes fuscipes, Ixodes longiscutatus and Ixodes pararicinus from the Southern Cone of America in northern Argentina, southern Brazil and Uruguay. The results of this work suggest that the haplotypes of B. burgdorferi s. l. complex detected in the three species of the I. ricinus complex distributed in the Southern Cone of America are related and widely distributed. EEA Rafaela Fil: Flores, Fernando S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria; Uruguay Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-04T20:48:58Z 2020-09-04T20:48:58Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7827 https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304155 1877-959X https://doi.org/10.1016/j.ttbdis.2020.101546 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Ticks and Tick-borne Diseases 11 (6) : 101546 (November 2020)
spellingShingle Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
Flores, Fernando Sebastián
Saracho Bottero, Maria Noelia
Sebastian, Patrick Stephan
Venzal, José Manuel
Mangold, Atilio Jose
Nava, Santiago
Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_full Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_fullStr Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_full_unstemmed Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_short Borrelia genospecies in Ixodes sp. cf. Ixodes affinis (Acari: Ixodidae) from Argentina
title_sort borrelia genospecies in ixodes sp cf ixodes affinis acari ixodidae from argentina
topic Ixodes
Ixodes ricinus
Identificación
Argentina
Identification
Garrapatas
Borrelia
Ticks
url http://hdl.handle.net/20.500.12123/7827
https://www.sciencedirect.com/science/article/abs/pii/S1877959X20304155
https://doi.org/10.1016/j.ttbdis.2020.101546
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