Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system
The role of the local innate immune response in the neuropathogenesis of bovine herpesvirus (BoHV) type 1 and 5 remains largely unknown. This study determined the gene transcriptional expression of relevant bovine cathelicidins, TNFα and IFNβ in the nervous system of experimentally-infected cattle d...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7790 https://www.sciencedirect.com/science/article/abs/pii/S0161589020301620 https://doi.org/10.1016/j.molimm.2020.04.017 |
| _version_ | 1855035921232035840 |
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| author | Burucúa, Mercedes María Perez, Sandra Odeon, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange |
| author_browse | Burucúa, Mercedes María Cobo, Eduardo Ruben Marin, Maia Solange Odeon, Anselmo Carlos Perez, Sandra Quintana, Silvina |
| author_facet | Burucúa, Mercedes María Perez, Sandra Odeon, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange |
| author_sort | Burucúa, Mercedes María |
| collection | INTA Digital |
| description | The role of the local innate immune response in the neuropathogenesis of bovine herpesvirus (BoHV) type 1 and 5 remains largely unknown. This study determined the gene transcriptional expression of relevant bovine cathelicidins, TNFα and IFNβ in the nervous system of experimentally-infected cattle during the different stages of BoHV-1 and BoHV-5 infectious cycle. We studied the modulation of bovine myeloid antimicrobial peptide (BMAP) 27 and 28 by alpha-herpesviruses during acute infection of the central nervous system (CNS). However, BMAP 28 was the main cathelicidin modulated.. BoHV-5 supressed BMAP28 expression mainly in frontal cortex and cervical medulla whereas BoHV-1 slightly induced the expression of cathelicidins in the olfactory and posterior cortex. The differences in the regulation of the innate response are likely related to distinct replication rates of both alpha-herpesviruses in the CNS. During latency and reactivation, BoHV-1 and -5 decreased BMAP28 and BMAP27 expression, accompanied by high levels of TNFα and IFNβ transcripts in the posterior brain region and medulla during BoHV reactivation. In terms of cytokines, a remarkably overexpression of IFNβ was induced by BoHV-5 (133.8-fold). In trigeminal ganglion (TG) both alpha-herpesviruses induced cathelidicins gene expression at all stages of the infection cycle, while only acute BoHV-5 infection increased TNFα (129-fold) mRNA levels. This study suggests that the pronounced downregulation of BMAP28 in BoHV-5-acutely-infected CNS is due to a decreased immune stimulation during viral infection, favouring its establishment in the CNS with a low replication rate until latency. Thus, cathelicidins, together with IFNβ and TNFα, are differentially regulated by BoHV-5 and BoHV-1 infections and this regulation is dependent on the stage of virus infection in the bovine nervous system. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7790 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77902020-08-31T11:30:36Z Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system Burucúa, Mercedes María Perez, Sandra Odeon, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange Bovina Herpes Virus Bovino Neuropéptidos Respuesta Inmunológica Sistema Nervioso Bovinae Bovine Herpesvirus Neuropeptides Immune Response Nervous System The role of the local innate immune response in the neuropathogenesis of bovine herpesvirus (BoHV) type 1 and 5 remains largely unknown. This study determined the gene transcriptional expression of relevant bovine cathelicidins, TNFα and IFNβ in the nervous system of experimentally-infected cattle during the different stages of BoHV-1 and BoHV-5 infectious cycle. We studied the modulation of bovine myeloid antimicrobial peptide (BMAP) 27 and 28 by alpha-herpesviruses during acute infection of the central nervous system (CNS). However, BMAP 28 was the main cathelicidin modulated.. BoHV-5 supressed BMAP28 expression mainly in frontal cortex and cervical medulla whereas BoHV-1 slightly induced the expression of cathelicidins in the olfactory and posterior cortex. The differences in the regulation of the innate response are likely related to distinct replication rates of both alpha-herpesviruses in the CNS. During latency and reactivation, BoHV-1 and -5 decreased BMAP28 and BMAP27 expression, accompanied by high levels of TNFα and IFNβ transcripts in the posterior brain region and medulla during BoHV reactivation. In terms of cytokines, a remarkably overexpression of IFNβ was induced by BoHV-5 (133.8-fold). In trigeminal ganglion (TG) both alpha-herpesviruses induced cathelidicins gene expression at all stages of the infection cycle, while only acute BoHV-5 infection increased TNFα (129-fold) mRNA levels. This study suggests that the pronounced downregulation of BMAP28 in BoHV-5-acutely-infected CNS is due to a decreased immune stimulation during viral infection, favouring its establishment in the CNS with a low replication rate until latency. Thus, cathelicidins, together with IFNβ and TNFα, are differentially regulated by BoHV-5 and BoHV-1 infections and this regulation is dependent on the stage of virus infection in the bovine nervous system. EEA Balcarce Fil: Burucúa, Mercedes María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Odeón Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine; Canadá Fil: Quintana, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. 2020-08-31T11:21:36Z 2020-08-31T11:21:36Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7790 https://www.sciencedirect.com/science/article/abs/pii/S0161589020301620 0161-5890 1872-9142 https://doi.org/10.1016/j.molimm.2020.04.017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Molecular Immunology 122 : 148-155 (2020) |
| spellingShingle | Bovina Herpes Virus Bovino Neuropéptidos Respuesta Inmunológica Sistema Nervioso Bovinae Bovine Herpesvirus Neuropeptides Immune Response Nervous System Burucúa, Mercedes María Perez, Sandra Odeon, Anselmo Carlos Cobo, Eduardo Ruben Quintana, Silvina Marin, Maia Solange Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title | Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title_full | Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title_fullStr | Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title_full_unstemmed | Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title_short | Cathelicidin bovine myeloid antimicrobial peptide (BMAP) 28 is involved in the inflammatory response against alpha-herpesviruses in the bovine nervous system |
| title_sort | cathelicidin bovine myeloid antimicrobial peptide bmap 28 is involved in the inflammatory response against alpha herpesviruses in the bovine nervous system |
| topic | Bovina Herpes Virus Bovino Neuropéptidos Respuesta Inmunológica Sistema Nervioso Bovinae Bovine Herpesvirus Neuropeptides Immune Response Nervous System |
| url | http://hdl.handle.net/20.500.12123/7790 https://www.sciencedirect.com/science/article/abs/pii/S0161589020301620 https://doi.org/10.1016/j.molimm.2020.04.017 |
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