Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobact...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7787 https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034 https://doi.org/10.1016/j.tvjl.2020.105426 |
| _version_ | 1855035920675241984 |
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| author | Griffa, Natanael Moyano, Roberto Damián Canal, Ana María Traveria, Gabriel Eduardo Santangelo, María De La Paz Alonso, Maria Natalia Romano, Maria Isabel |
| author_browse | Alonso, Maria Natalia Canal, Ana María Griffa, Natanael Moyano, Roberto Damián Romano, Maria Isabel Santangelo, María De La Paz Traveria, Gabriel Eduardo |
| author_facet | Griffa, Natanael Moyano, Roberto Damián Canal, Ana María Traveria, Gabriel Eduardo Santangelo, María De La Paz Alonso, Maria Natalia Romano, Maria Isabel |
| author_sort | Griffa, Natanael |
| collection | INTA Digital |
| description | Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina.
The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7787 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77872020-08-28T16:52:04Z Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis Griffa, Natanael Moyano, Roberto Damián Canal, Ana María Traveria, Gabriel Eduardo Santangelo, María De La Paz Alonso, Maria Natalia Romano, Maria Isabel Tuberculosis Infeccion por Mycobacterium bovis ELISA Mycobacterium bovis Infections Tests Humoral Response Respuesta Humoral Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health. Instituto de Biotecnologìa Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Traveria, Gabriel Eduardo. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-08-28T16:46:14Z 2020-08-28T16:46:14Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7787 https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034 1090-0233 https://doi.org/10.1016/j.tvjl.2020.105426 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier The Veterinary Journal 256 : 105426 (Febrero 2020) |
| spellingShingle | Tuberculosis Infeccion por Mycobacterium bovis ELISA Mycobacterium bovis Infections Tests Humoral Response Respuesta Humoral Griffa, Natanael Moyano, Roberto Damián Canal, Ana María Traveria, Gabriel Eduardo Santangelo, María De La Paz Alonso, Maria Natalia Romano, Maria Isabel Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title | Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title_full | Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title_fullStr | Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title_full_unstemmed | Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title_short | Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis |
| title_sort | development and diagnostic validation of an elisa based on an antigenic mixture for the detection of bovine tuberculosis |
| topic | Tuberculosis Infeccion por Mycobacterium bovis ELISA Mycobacterium bovis Infections Tests Humoral Response Respuesta Humoral |
| url | http://hdl.handle.net/20.500.12123/7787 https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034 https://doi.org/10.1016/j.tvjl.2020.105426 |
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