Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein
Bacillus thuringiensis toxins of the Cry1I class have dual specificity for insects in the orders Coleoptera and Lepidoptera. We assessed the toxicity of a Cry1Ia protein from an Argentinian B. thuringiensis strain against agricultural pests in the families Tenebrionidae, Curculionidae, Noctuidae and...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7784 https://www.sciencedirect.com/science/article/abs/pii/S0022201120300604 https://doi.org/10.1016/j.jip.2020.107354 |
| _version_ | 1855484074182836224 |
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| author | Berretta, Marcelo Facundo Pedarros, Analía Soledad Sauka, Diego Hernan Perez, Melisa Paula Onco, Maria Ines Benintende, Graciela Beatriz |
| author_browse | Benintende, Graciela Beatriz Berretta, Marcelo Facundo Onco, Maria Ines Pedarros, Analía Soledad Perez, Melisa Paula Sauka, Diego Hernan |
| author_facet | Berretta, Marcelo Facundo Pedarros, Analía Soledad Sauka, Diego Hernan Perez, Melisa Paula Onco, Maria Ines Benintende, Graciela Beatriz |
| author_sort | Berretta, Marcelo Facundo |
| collection | INTA Digital |
| description | Bacillus thuringiensis toxins of the Cry1I class have dual specificity for insects in the orders Coleoptera and Lepidoptera. We assessed the toxicity of a Cry1Ia protein from an Argentinian B. thuringiensis strain against agricultural pests in the families Tenebrionidae, Curculionidae, Noctuidae and Tortricidae. Three recombinant protein variants were produced that differed in length and fusion tag position to rule out artifactual results. The protein was toxic to Cydia pomonella and Rachiplusia nu. In contrast, Alphitobius diaperinus, Anthonomus grandis and Spodoptera frugiperda were not susceptible. The results are discussed with respect to previous studies and the prospective use of Cry1Ia in strategies to control major cotton pests in the region. |
| format | Artículo |
| id | INTA7784 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77842020-08-28T13:25:08Z Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein Berretta, Marcelo Facundo Pedarros, Analía Soledad Sauka, Diego Hernan Perez, Melisa Paula Onco, Maria Ines Benintende, Graciela Beatriz Bacillus thuringiensis Plagas de Plantas Control de Plagas América del Sur Pests of Plants Pest Control South America Cry1Ia Bacillus thuringiensis toxins of the Cry1I class have dual specificity for insects in the orders Coleoptera and Lepidoptera. We assessed the toxicity of a Cry1Ia protein from an Argentinian B. thuringiensis strain against agricultural pests in the families Tenebrionidae, Curculionidae, Noctuidae and Tortricidae. Three recombinant protein variants were produced that differed in length and fusion tag position to rule out artifactual results. The protein was toxic to Cydia pomonella and Rachiplusia nu. In contrast, Alphitobius diaperinus, Anthonomus grandis and Spodoptera frugiperda were not susceptible. The results are discussed with respect to previous studies and the prospective use of Cry1Ia in strategies to control major cotton pests in the region. Instituto de Microbiología y Zoología Agrícola Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola e Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pedarros, Analía Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología; Argentina Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología e Instituto de Biotecnología; Argentina Fil: Perez, Melisa Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología e Instituto de Biotecnología; Argentina Fil: Onco, Maria Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología; Argentina 2020-08-28T13:21:07Z 2020-08-28T13:21:07Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7784 https://www.sciencedirect.com/science/article/abs/pii/S0022201120300604 0022-2011 https://doi.org/10.1016/j.jip.2020.107354 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Invertebrate Pathology 172 : 107354 (May 2020) |
| spellingShingle | Bacillus thuringiensis Plagas de Plantas Control de Plagas América del Sur Pests of Plants Pest Control South America Cry1Ia Berretta, Marcelo Facundo Pedarros, Analía Soledad Sauka, Diego Hernan Perez, Melisa Paula Onco, Maria Ines Benintende, Graciela Beatriz Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title | Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title_full | Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title_fullStr | Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title_full_unstemmed | Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title_short | Susceptibility of agricultural pests of regional importance in South America to a Bacillus thuringiensis Cry1Ia protein |
| title_sort | susceptibility of agricultural pests of regional importance in south america to a bacillus thuringiensis cry1ia protein |
| topic | Bacillus thuringiensis Plagas de Plantas Control de Plagas América del Sur Pests of Plants Pest Control South America Cry1Ia |
| url | http://hdl.handle.net/20.500.12123/7784 https://www.sciencedirect.com/science/article/abs/pii/S0022201120300604 https://doi.org/10.1016/j.jip.2020.107354 |
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