Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus
The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn wa...
| Main Authors: | , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Springer
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7783 https://link.springer.com/article/10.1007/s11262-020-01741-9 https://doi.org/10.1007/s11262-020-01741-9 |
| _version_ | 1855035919949627392 |
|---|---|
| author | Niz, José María Salvador, Ricardo Ferrelli, María Leticia Sciocco, Alicia Ines Romanowski, Víctor Berretta, Marcelo Facundo |
| author_browse | Berretta, Marcelo Facundo Ferrelli, María Leticia Niz, José María Romanowski, Víctor Salvador, Ricardo Sciocco, Alicia Ines |
| author_facet | Niz, José María Salvador, Ricardo Ferrelli, María Leticia Sciocco, Alicia Ines Romanowski, Víctor Berretta, Marcelo Facundo |
| author_sort | Niz, José María |
| collection | INTA Digital |
| description | The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7783 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA77832020-08-28T12:47:13Z Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Niz, José María Salvador, Ricardo Ferrelli, María Leticia Sciocco, Alicia Ines Romanowski, Víctor Berretta, Marcelo Facundo Spodoptera frugiperda Plagas de Plantas Control Biológico Baculovirus Pests of Plants Biological Control The fall armyworm, Spodoptera frugiperda (JE Smith) is a key pest in the Americas. Control strategies are mainly carried out by use of chemical insecticides and transgenic crops expressing Bacillus thuringiensis toxins. In the last years, resistance of S. frugiperda populations to transgenic corn was reported in different Latin American countries. The baculovirus Spodoptera frugiperda Multiple Nucleopolyhedrovirus (SfMNPV) is a pathogenic agent for the fall armyworm and a potential alternative for its control in integrated pest management strategies. In this work, we analyze some characteristics of two baculovirus isolates collected from maize (SfMNPV-M) and cotton (SfMNPV-C) fields from Argentina. The isolates were compared by restriction enzymes patterns and the analysis reveals the presence of genotypic variants in the SfMNPV-M isolate. We confirmed a deletion by sequencing fragments encompassing egt gene and most part of its contiguous gene (orf A) in a SfMNVP-M genotypic variant. Additionally, we estimated the 50% lethal dose and median survival time of each isolate in bioassays with S. frugiperda larvae. Instituto de Microbiología y Zoología Agrícola Fil: Niz, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Ferrelli, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Romanowski, Víctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola e Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-08-28T12:39:52Z 2020-08-28T12:39:52Z 2020 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7783 https://link.springer.com/article/10.1007/s11262-020-01741-9 0920-8569 1572-994X https://doi.org/10.1007/s11262-020-01741-9 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Virus Genes 56 : 401–405 (2020) |
| spellingShingle | Spodoptera frugiperda Plagas de Plantas Control Biológico Baculovirus Pests of Plants Biological Control Niz, José María Salvador, Ricardo Ferrelli, María Leticia Sciocco, Alicia Ines Romanowski, Víctor Berretta, Marcelo Facundo Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title | Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title_full | Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title_fullStr | Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title_full_unstemmed | Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title_short | Genetic variants in Argentinean isolates of Spodoptera frugiperda Multiple Nucleopolyhedrovirus |
| title_sort | genetic variants in argentinean isolates of spodoptera frugiperda multiple nucleopolyhedrovirus |
| topic | Spodoptera frugiperda Plagas de Plantas Control Biológico Baculovirus Pests of Plants Biological Control |
| url | http://hdl.handle.net/20.500.12123/7783 https://link.springer.com/article/10.1007/s11262-020-01741-9 https://doi.org/10.1007/s11262-020-01741-9 |
| work_keys_str_mv | AT nizjosemaria geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus AT salvadorricardo geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus AT ferrellimarialeticia geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus AT scioccoaliciaines geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus AT romanowskivictor geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus AT berrettamarcelofacundo geneticvariantsinargentineanisolatesofspodopterafrugiperdamultiplenucleopolyhedrovirus |