Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina
Bovine babesiosis is a tick‐transmitted haemoparasitic disease caused by Babesia bovis and B. bigemina affecting cattle of tropical and subtropical regions around the world. Pathogens are transmitted by the tick vector Rhipicephalus microplus displaying a widespread distribution in northeastern Arge...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7780 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13435 https://doi.org/10.1111/tbed.13435 |
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| author | Ganzinelli, Sabrina Belen Benitez, Daniel Francisco Gantuya, Sambuu Guswanto, Azirwan Florin-Christensen, Mónica Schnittger, Leonhard Igarashi, Ikuo |
| author_browse | Benitez, Daniel Francisco Florin-Christensen, Mónica Gantuya, Sambuu Ganzinelli, Sabrina Belen Guswanto, Azirwan Igarashi, Ikuo Schnittger, Leonhard |
| author_facet | Ganzinelli, Sabrina Belen Benitez, Daniel Francisco Gantuya, Sambuu Guswanto, Azirwan Florin-Christensen, Mónica Schnittger, Leonhard Igarashi, Ikuo |
| author_sort | Ganzinelli, Sabrina Belen |
| collection | INTA Digital |
| description | Bovine babesiosis is a tick‐transmitted haemoparasitic disease caused by Babesia bovis and B. bigemina affecting cattle of tropical and subtropical regions around the world. Pathogens are transmitted by the tick vector Rhipicephalus microplus displaying a widespread distribution in northeastern Argentina. The disease is characterized by significant animal morbidity and mortality resulting in considerable economic loss. In this study, B. bovis and B. bigemina infection was investigated in a cattle herd of 150 adult bovines of pure Braford breed raised in a tick‐hyperendemic field using molecular and serum antibody tests. A highly sensitive nested polymerase chain reaction (nPCR) assay targeting a species‐specific region of the apocytochrome b gene resulted in direct B. bovis and B. bigemina detection in 27.3% and 54.7% of bovines, respectively. A recently developed immunochromatographic strip test (ICT) based on recombinant forms of spherical body protein 4 and the C‐terminal region of rhoptry‐associated protein 1 showed that 71.3% and 89.3% of bovines were seropositive for B. bovis and B. bigemina, respectively. The mixed infection rate as observed by direct (19.3%) and indirect detection (65.3%) coincided with those expected, respectively. Importantly, four months after sampling, nine bovines of the studied herd showed clinical signs of bovine babesiosis of which six animals eventually died. Microscopic detection of infected erythrocytes in Giemsa‐stained blood smears confirmed B. bovis infection. Our study demonstrates that although animals showed a relatively high and very high rate of immunity against infection with B. bovis (71.3%) and B. bigemina (89.3%) parasites, respectively, clinical cases and fatalities due to the infection with B. bovis were observed. It is proposed that the most adequate control measure in the studied epidemiological situation is to vaccinate animals to prevent losses and/or an outbreak of bovine babesiosis. |
| format | Artículo |
| id | INTA7780 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA77802020-08-27T17:40:24Z Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina Ganzinelli, Sabrina Belen Benitez, Daniel Francisco Gantuya, Sambuu Guswanto, Azirwan Florin-Christensen, Mónica Schnittger, Leonhard Igarashi, Ikuo Babesia bigemina Babesia bovis Babesiosis Inmunodiagnóstico Ganado Bovino Argentina PCR Immunodiagnosis Cattle Bovine babesiosis is a tick‐transmitted haemoparasitic disease caused by Babesia bovis and B. bigemina affecting cattle of tropical and subtropical regions around the world. Pathogens are transmitted by the tick vector Rhipicephalus microplus displaying a widespread distribution in northeastern Argentina. The disease is characterized by significant animal morbidity and mortality resulting in considerable economic loss. In this study, B. bovis and B. bigemina infection was investigated in a cattle herd of 150 adult bovines of pure Braford breed raised in a tick‐hyperendemic field using molecular and serum antibody tests. A highly sensitive nested polymerase chain reaction (nPCR) assay targeting a species‐specific region of the apocytochrome b gene resulted in direct B. bovis and B. bigemina detection in 27.3% and 54.7% of bovines, respectively. A recently developed immunochromatographic strip test (ICT) based on recombinant forms of spherical body protein 4 and the C‐terminal region of rhoptry‐associated protein 1 showed that 71.3% and 89.3% of bovines were seropositive for B. bovis and B. bigemina, respectively. The mixed infection rate as observed by direct (19.3%) and indirect detection (65.3%) coincided with those expected, respectively. Importantly, four months after sampling, nine bovines of the studied herd showed clinical signs of bovine babesiosis of which six animals eventually died. Microscopic detection of infected erythrocytes in Giemsa‐stained blood smears confirmed B. bovis infection. Our study demonstrates that although animals showed a relatively high and very high rate of immunity against infection with B. bovis (71.3%) and B. bigemina (89.3%) parasites, respectively, clinical cases and fatalities due to the infection with B. bovis were observed. It is proposed that the most adequate control measure in the studied epidemiological situation is to vaccinate animals to prevent losses and/or an outbreak of bovine babesiosis. Instituto de Patobiología Fil: Ganzinelli Sabrina Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Benitez, Daniel Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Gantuya, Sambuu. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japón Fil: Guswanto, Azirwan. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japón Fil: Florin-Christensen, Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Igarashi, Ikuo. Obihiro University of Agriculture and Veterinary Medicine. National Research Center for Protozoan Diseases; Japón 2020-08-27T17:35:35Z 2020-08-27T17:35:35Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7780 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13435 1865-1674 https://doi.org/10.1111/tbed.13435 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Transboundary and Emerging Diseases 67 (Supl. 2) : 159-164 (Julio 2020) |
| spellingShingle | Babesia bigemina Babesia bovis Babesiosis Inmunodiagnóstico Ganado Bovino Argentina PCR Immunodiagnosis Cattle Ganzinelli, Sabrina Belen Benitez, Daniel Francisco Gantuya, Sambuu Guswanto, Azirwan Florin-Christensen, Mónica Schnittger, Leonhard Igarashi, Ikuo Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title | Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title_full | Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title_fullStr | Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title_full_unstemmed | Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title_short | Highly sensitive nested PCR and rapid immunochromatographic detection of Babesia bovis and Babesia bigemina infection in a cattle herd with acute clinical and fatal cases in Argentina |
| title_sort | highly sensitive nested pcr and rapid immunochromatographic detection of babesia bovis and babesia bigemina infection in a cattle herd with acute clinical and fatal cases in argentina |
| topic | Babesia bigemina Babesia bovis Babesiosis Inmunodiagnóstico Ganado Bovino Argentina PCR Immunodiagnosis Cattle |
| url | http://hdl.handle.net/20.500.12123/7780 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13435 https://doi.org/10.1111/tbed.13435 |
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