Development of diagnostic tools for IBDV detection using plants as bioreactors
Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been...
| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
SpringerOpen
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7766 https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z https://doi.org/10.1186/s13568-020-01029-z |
| _version_ | 1855484070959513600 |
|---|---|
| author | Gomez, Evangelina Raquel Cassani, María Florencia Lucero, Maria Soledad Parreño, Viviana Chimeno Zoth, Silvina Andrea Berinstein, Analia |
| author_browse | Berinstein, Analia Cassani, María Florencia Chimeno Zoth, Silvina Andrea Gomez, Evangelina Raquel Lucero, Maria Soledad Parreño, Viviana |
| author_facet | Gomez, Evangelina Raquel Cassani, María Florencia Lucero, Maria Soledad Parreño, Viviana Chimeno Zoth, Silvina Andrea Berinstein, Analia |
| author_sort | Gomez, Evangelina Raquel |
| collection | INTA Digital |
| description | Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease. |
| format | Artículo |
| id | INTA7766 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | SpringerOpen |
| publisherStr | SpringerOpen |
| record_format | dspace |
| spelling | INTA77662020-08-25T12:22:00Z Development of diagnostic tools for IBDV detection using plants as bioreactors Gomez, Evangelina Raquel Cassani, María Florencia Lucero, Maria Soledad Parreño, Viviana Chimeno Zoth, Silvina Andrea Berinstein, Analia Enfermedades de los Animales Virus Bursitis Infecciosa Diagnóstico Biorreactores Animal Diseases Infectious Bursal Disease Virus Diagnosis Bioreactors Nicotiana benthamiana Infectious bursal disease virus (IBDV) is the etiological agent of an immunosuppressive and highly contagious disease that affects young birds, thus causing important economic losses in the poultry industry. Multimeric particles with different architectures based on the capsid protein VP2 have been widely produced for different purposes. We hereby show the production and easy recovery of IBDV subviral particles (SVP) from transiently transformed Nicotiana benthamiana. The SVP, which were observed by electronic microscopy, proved to be antigenically and immunogenically similar to the virion. Indeed, anti-IBDV antibodies from samples of infected birds recognized these SVP and, when injected intramuscularly, these subviral particles also evoked a humoral immune response in chickens. We developed an in-house ELISA using SVP as coating reagent that demonstrated to be highly accurate and in good agreement with a commercial ELISA. This study demonstrates that the recombinant antigen generated and the technology used to produce it are suitable for developing a diagnostic tool against Infectious bursal disease. Instituto de Biotecnología Fil: Gomez, Evangelina Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cassani, María Florencia. Universidad Nacional de Luján; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Lucero, Maria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Berinstein, Analia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina 2020-08-25T12:18:32Z 2020-08-25T12:18:32Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7766 https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z 2191-0855 https://doi.org/10.1186/s13568-020-01029-z eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf SpringerOpen AMB Express 10 : article number: 95 (2020) |
| spellingShingle | Enfermedades de los Animales Virus Bursitis Infecciosa Diagnóstico Biorreactores Animal Diseases Infectious Bursal Disease Virus Diagnosis Bioreactors Nicotiana benthamiana Gomez, Evangelina Raquel Cassani, María Florencia Lucero, Maria Soledad Parreño, Viviana Chimeno Zoth, Silvina Andrea Berinstein, Analia Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title | Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title_full | Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title_fullStr | Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title_full_unstemmed | Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title_short | Development of diagnostic tools for IBDV detection using plants as bioreactors |
| title_sort | development of diagnostic tools for ibdv detection using plants as bioreactors |
| topic | Enfermedades de los Animales Virus Bursitis Infecciosa Diagnóstico Biorreactores Animal Diseases Infectious Bursal Disease Virus Diagnosis Bioreactors Nicotiana benthamiana |
| url | http://hdl.handle.net/20.500.12123/7766 https://amb-express.springeropen.com/articles/10.1186/s13568-020-01029-z https://doi.org/10.1186/s13568-020-01029-z |
| work_keys_str_mv | AT gomezevangelinaraquel developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors AT cassanimariaflorencia developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors AT luceromariasoledad developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors AT parrenoviviana developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors AT chimenozothsilvinaandrea developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors AT berinsteinanalia developmentofdiagnostictoolsforibdvdetectionusingplantsasbioreactors |