Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana
Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses....
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7759 https://link.springer.com/article/10.1007/s10658-020-02054-1 https://doi.org/10.1007/s10658-020-02054-1 |
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| author | Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel |
| author_browse | Díaz Ricci, Juan Carlos Hael Conrad, Veronica Moschen, Sebastian Nicolas Requena-Serra, F.J. Salazar, Sergio Miguel Tomas-Grau, Rodrigo H. |
| author_facet | Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel |
| author_sort | Hael Conrad, Veronica |
| collection | INTA Digital |
| description | Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF. |
| format | Artículo |
| id | INTA7759 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA77592020-08-24T13:24:24Z Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts Development of a sustainable agriculture is a current trend searching for alternative and more environmentally friendly strategies to control crop diseases. The necrotrophic fungus Botrytis cinerea, the causal agent of grey mould, affects crops pre- and post-harvest and cause huge economical losses. Microorganisms and microbially-derived elicitors are known to induce plant defences. Here, we study two fungal extracts derived from Colletotrichum acutatum (isolate M11). The crude extract, called CF (culture filtrate) and the semi-purified fraction ACF (axenic culture filtrate) consist in a blend of fungal-derived elicitors. Their activities were evaluated in the plant-pathogen system Arabidopsis thaliana-B. cinerea. Both CF and ACF induced local and systemic resistance, rendering A. thaliana resistant against grey mould disease. The extracts showed no antifungal activity since no inhibition of mycelium growth, changes in hyphal ultrastructure or damage in cell membrane permeability were observed. These findings would indicate that the protective effect was indirect and it was found that CF and ACF induced up-regulation of the defence-related genes PR1, WRKY70 and ERF6, as well as giving local callose deposition in treated leaves. Both fungal extracts have the potential to be used as a main component of a biologically based disease control product with activity against grey mould, with the advantage that the purification process of CF is simpler than for ACF. EEA Famaillá Fil: Hael-Conrad, Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Tomas-Grau, R.H. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Requena-Serra, F.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina Fil: Diaz Ricci, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina. Fil: Salazar, Sergio Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Universidad Nacional de Tucumán. Facultad de Agronomía y Zootecnia; Argentina 2020-08-24T13:07:40Z 2020-08-24T13:07:40Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7759 https://link.springer.com/article/10.1007/s10658-020-02054-1 0929-1873 1573-8469 https://doi.org/10.1007/s10658-020-02054-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology 158 : 45–58 (2020) |
| spellingShingle | Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts Hael Conrad, Veronica Tomas-Grau, Rodrigo H. Moschen, Sebastian Nicolas Requena-Serra, F.J. Díaz Ricci, Juan Carlos Salazar, Sergio Miguel Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title | Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_full | Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_fullStr | Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_full_unstemmed | Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_short | Fungal-derived extracts induce resistance against Botrytis cinerea in Arabidopsis thaliana |
| title_sort | fungal derived extracts induce resistance against botrytis cinerea in arabidopsis thaliana |
| topic | Enfermedades de las Plantas Control de Enfermedades Botrytis cinerea Arabidopsis thaliana Extractos Plant Diseases Diseases Control Extracts |
| url | http://hdl.handle.net/20.500.12123/7759 https://link.springer.com/article/10.1007/s10658-020-02054-1 https://doi.org/10.1007/s10658-020-02054-1 |
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