Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7745 https://www.sciencedirect.com/science/article/pii/S0145305X19305178 https://doi.org/10.1016/j.dci.2019.103606 |
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| author | Pavanelo, Daniel Brisotto Schröder, Nicolas C.H. Pin Viso, Natalia Daniela Martins, Larissa A. Malossi, Camila Dantas Galletti, Maria Fernanda B.M. Labruna, Marcelo B. Daffre, Sirlei Farber, Marisa Diana Fogaça, Andréa C. |
| author_browse | Daffre, Sirlei Farber, Marisa Diana Fogaça, Andréa C. Galletti, Maria Fernanda B.M. Labruna, Marcelo B. Malossi, Camila Dantas Martins, Larissa A. Pavanelo, Daniel Brisotto Pin Viso, Natalia Daniela Schröder, Nicolas C.H. |
| author_facet | Pavanelo, Daniel Brisotto Schröder, Nicolas C.H. Pin Viso, Natalia Daniela Martins, Larissa A. Malossi, Camila Dantas Galletti, Maria Fernanda B.M. Labruna, Marcelo B. Daffre, Sirlei Farber, Marisa Diana Fogaça, Andréa C. |
| author_sort | Pavanelo, Daniel Brisotto |
| collection | INTA Digital |
| description | Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission. |
| format | Artículo |
| id | INTA7745 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77452020-08-20T13:23:13Z Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii Pavanelo, Daniel Brisotto Schröder, Nicolas C.H. Pin Viso, Natalia Daniela Martins, Larissa A. Malossi, Camila Dantas Galletti, Maria Fernanda B.M. Labruna, Marcelo B. Daffre, Sirlei Farber, Marisa Diana Fogaça, Andréa C. Rickettsia Vectores Relaciones Huésped Parásito Flora Microbiana Vectors Host Parasite Relations Microbial Flora Garrapatas Rickettsia rickettsii Microbiota Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission. Instituto de Biotecnología Fil: Pavanelo, Daniel Brisotto. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Schröder, Nicolas C.H. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Martins, Larissa A. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Malossi, Camila Dantas. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Galletti, Maria Fernanda B.M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Labruna, Marcelo B. University of São Paulo. Faculty of Veterinary Medicine and Husbandry. Department of Preventive Veterinary Medicine and Animal Health; Brasil Fil: Daffre, Sirlei. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Fogaça, Andréa C. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil 2020-08-20T13:17:47Z 2020-08-20T13:17:47Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7745 https://www.sciencedirect.com/science/article/pii/S0145305X19305178 0145-305X https://doi.org/10.1016/j.dci.2019.103606 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Developmental & Comparative Immunology 106 : 103606 (May 2020) |
| spellingShingle | Rickettsia Vectores Relaciones Huésped Parásito Flora Microbiana Vectors Host Parasite Relations Microbial Flora Garrapatas Rickettsia rickettsii Microbiota Pavanelo, Daniel Brisotto Schröder, Nicolas C.H. Pin Viso, Natalia Daniela Martins, Larissa A. Malossi, Camila Dantas Galletti, Maria Fernanda B.M. Labruna, Marcelo B. Daffre, Sirlei Farber, Marisa Diana Fogaça, Andréa C. Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title | Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title_full | Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title_fullStr | Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title_full_unstemmed | Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title_short | Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii |
| title_sort | comparative analysis of the midgut microbiota of two natural tick vectors of rickettsia rickettsii |
| topic | Rickettsia Vectores Relaciones Huésped Parásito Flora Microbiana Vectors Host Parasite Relations Microbial Flora Garrapatas Rickettsia rickettsii Microbiota |
| url | http://hdl.handle.net/20.500.12123/7745 https://www.sciencedirect.com/science/article/pii/S0145305X19305178 https://doi.org/10.1016/j.dci.2019.103606 |
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