A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases
Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and...
| Main Authors: | , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Elsevier
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7737 https://www.sciencedirect.com/science/article/abs/pii/S1877959X19304042 https://doi.org/10.1016/j.ttbdis.2020.101374 |
| _version_ | 1855035912011907072 |
|---|---|
| author | Lu, Stephen Rocha, Leticia A. da Torquato, Ricardo J.S. Vaz Junior, Itabajara da Silva Florin-Christensen, Mónica Tanaka, Aparecida S. |
| author_browse | Florin-Christensen, Mónica Lu, Stephen Rocha, Leticia A. da Tanaka, Aparecida S. Torquato, Ricardo J.S. Vaz Junior, Itabajara da Silva |
| author_facet | Lu, Stephen Rocha, Leticia A. da Torquato, Ricardo J.S. Vaz Junior, Itabajara da Silva Florin-Christensen, Mónica Tanaka, Aparecida S. |
| author_sort | Lu, Stephen |
| collection | INTA Digital |
| description | Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7737 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77372020-08-19T13:31:43Z A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases Lu, Stephen Rocha, Leticia A. da Torquato, Ricardo J.S. Vaz Junior, Itabajara da Silva Florin-Christensen, Mónica Tanaka, Aparecida S. Rhipicephalus Ganado Bovino Cisteína Cattle Cysteine Garrapatas Rhipicephalus (Boophilus) microplus Cystatin Rhipicephalus microplus is a cattle ectoparasite found in tropical and subtropical regions around the world with great impact on livestock production. R. microplus can also harbor pathogens, such as Babesia sp. and Anaplasma sp. which further compromise cattle production. Blood meal acquisition and digestion are key steps for tick development. In ticks, digestion takes place inside midgut cells and is mediated by aspartic and cysteine peptidases and, therefore, regulated by their inhibitors. Cystatins are a family of cysteine peptidases inhibitors found in several organisms and have been associated in ticks with blood acquisition, blood digestion, modulation of host immune response and tick immunity. In this work, we characterized a novel R. microplus type 1 cystatin, named Rmcystatin-1b. The inhibitor transcripts were found to be highly expressed in the midgut of partially and fully engorged females and they appear to be modulated at different days post-detachment. Purified recombinant Rmcystatin-1b displayed inhibitory activity towards typical cysteine peptidases with high affinity. Moreover, rRmcystatin-1b was able to inhibit native R. microplus cysteine peptidases and RNAi-mediated knockdown of the cystatin transcripts resulted in increased proteolytic activity. Moreover, rRmcystatin-1b was able to interfere with B. bovis growth in vitro. Taken together our data strongly suggest that Rmcystatin-1b is a regulator of blood digestion in R. microplus midgut. Instituto de Patobiología Fil: Lu, Stephen. Universidade de Federal de São Paulo. Escola Paulista de Medicina. Department of Biochemistry; Brasil Fil: Rocha, Leticia A. da. Universidade de Federal de São Paulo. Escola Paulista de Medicina. Department of Biochemistry; Brasil Fil: Torquato, Ricardo J.S. Universidade de Federal de São Paulo. Escola Paulista de Medicina. Department of Biochemistry; Brasil Fil: Vaz Junior, Itabajara da Silva. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia e Faculdade de Veterinária; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tanaka, Aparecida S. Universidade de Federal de São Paulo. Escola Paulista de Medicina. Department of Biochemistry; Brasil. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil 2020-08-19T13:27:22Z 2020-08-19T13:27:22Z 2020-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7737 https://www.sciencedirect.com/science/article/abs/pii/S1877959X19304042 1877-959X https://doi.org/10.1016/j.ttbdis.2020.101374 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Ticks and Tick-borne Diseases 11 (3) : 101374 (May 2020) |
| spellingShingle | Rhipicephalus Ganado Bovino Cisteína Cattle Cysteine Garrapatas Rhipicephalus (Boophilus) microplus Cystatin Lu, Stephen Rocha, Leticia A. da Torquato, Ricardo J.S. Vaz Junior, Itabajara da Silva Florin-Christensen, Mónica Tanaka, Aparecida S. A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title | A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title_full | A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title_fullStr | A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title_full_unstemmed | A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title_short | A novel type 1 cystatin involved in the regulation of Rhipicephalus microplus midgut cysteine proteases |
| title_sort | novel type 1 cystatin involved in the regulation of rhipicephalus microplus midgut cysteine proteases |
| topic | Rhipicephalus Ganado Bovino Cisteína Cattle Cysteine Garrapatas Rhipicephalus (Boophilus) microplus Cystatin |
| url | http://hdl.handle.net/20.500.12123/7737 https://www.sciencedirect.com/science/article/abs/pii/S1877959X19304042 https://doi.org/10.1016/j.ttbdis.2020.101374 |
| work_keys_str_mv | AT lustephen anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT rochaleticiaada anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT torquatoricardojs anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT vazjunioritabajaradasilva anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT florinchristensenmonica anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT tanakaaparecidas anoveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT lustephen noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT rochaleticiaada noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT torquatoricardojs noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT vazjunioritabajaradasilva noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT florinchristensenmonica noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases AT tanakaaparecidas noveltype1cystatininvolvedintheregulationofrhipicephalusmicroplusmidgutcysteineproteases |