Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina
Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, fie...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Elsevier
2020
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/7736 https://www.sciencedirect.com/science/article/abs/pii/S0034528820309255 https://doi.org/10.1016/j.rvsc.2020.07.012 |
| _version_ | 1855035911832600576 |
|---|---|
| author | Torrents, Jorgelina Sarli, Macarena Rossner, Maria Victoria Toffaletti, Jose Rodolfo Morel, Nicolas Martinez, Norberto Claudio Webster, Anelise Mangold, Atilio Jose Guglielmone, Alberto Nava, Santiago |
| author_browse | Guglielmone, Alberto Mangold, Atilio Jose Martinez, Norberto Claudio Morel, Nicolas Nava, Santiago Rossner, Maria Victoria Sarli, Macarena Toffaletti, Jose Rodolfo Torrents, Jorgelina Webster, Anelise |
| author_facet | Torrents, Jorgelina Sarli, Macarena Rossner, Maria Victoria Toffaletti, Jose Rodolfo Morel, Nicolas Martinez, Norberto Claudio Webster, Anelise Mangold, Atilio Jose Guglielmone, Alberto Nava, Santiago |
| author_sort | Torrents, Jorgelina |
| collection | INTA Digital |
| description | Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, field trials to control natural infestations of R. microplus on cattle with a commercial formulation of ivermectin 3.15% were carried and jointly analyzed with the in vitro assays. The phenotypic response of the populations analyzed was not uniform. Five of them were classified as susceptible, four populations as resistant, and one in the category “incipient resistance”. Regarding the field trials, the therapeutic efficacy in a population classified with LIT as susceptible achieved values higher than 94% two weeks after treatment, and no reproductively viable females were observed after the second day post-treatment. Conversely, the values of efficacy percentage in a population (named as “San Martín”) classified with LIT in the category “incipient resistance” never exceeded the 70.8%, and engorged females were collected in practically all counts. The population “San Martín” was classified in the category “incipient resistant” with LIT analysis, but the field trial unambiguously shows that this tick population is resistant. The comparison of the results obtained with LIT in vitro assays and through field trials shows that biased estimations of resistance levels may occur when resistance ratios (RR) values are ≤2, and additional field efficacy trials could be needed to know with precision the status of the tick populations evaluated. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7736 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77362020-08-19T12:36:26Z Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina Torrents, Jorgelina Sarli, Macarena Rossner, Maria Victoria Toffaletti, Jose Rodolfo Morel, Nicolas Martinez, Norberto Claudio Webster, Anelise Mangold, Atilio Jose Guglielmone, Alberto Nava, Santiago Ganado Bovino Rhipicephalus Plagas de Animales Control de Plagas Ivermectina Resistencia a los Acaricidas Argentina Cattle Pests of Animals Pest Control Ivermectin Resistance to Acaricides Garrapatas Rhipicephalus (Boophilus) microplus Resistance to ivermectin in populations of the cattle tick Rhipicephalus microplus in Argentina was diagnosed in this work. The in vitro larval immersion test (LIT) was used to determine quantitatively the levels of resistance to ivermectin in different populations of R. microplus. Additionally, field trials to control natural infestations of R. microplus on cattle with a commercial formulation of ivermectin 3.15% were carried and jointly analyzed with the in vitro assays. The phenotypic response of the populations analyzed was not uniform. Five of them were classified as susceptible, four populations as resistant, and one in the category “incipient resistance”. Regarding the field trials, the therapeutic efficacy in a population classified with LIT as susceptible achieved values higher than 94% two weeks after treatment, and no reproductively viable females were observed after the second day post-treatment. Conversely, the values of efficacy percentage in a population (named as “San Martín”) classified with LIT in the category “incipient resistance” never exceeded the 70.8%, and engorged females were collected in practically all counts. The population “San Martín” was classified in the category “incipient resistant” with LIT analysis, but the field trial unambiguously shows that this tick population is resistant. The comparison of the results obtained with LIT in vitro assays and through field trials shows that biased estimations of resistance levels may occur when resistance ratios (RR) values are ≤2, and additional field efficacy trials could be needed to know with precision the status of the tick populations evaluated. EEA Rafaela Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Rossner, Maria Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Colonia Benitez; Argentina Fil: Toffaletti, Jose Rodolfo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria El Colorado; Argentina Fil: Morel, Nicolas. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Martinez, Norberto Claudio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista. Agencia De Extensión Rural Garabato; Argentina Fil: Webster, Anelise. Instituto de Pesquisas Veterinárias Desidério Finamor; Brasil Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Guglielmone, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-08-19T12:28:21Z 2020-08-19T12:28:21Z 2020 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7736 https://www.sciencedirect.com/science/article/abs/pii/S0034528820309255 0034-5288 https://doi.org/10.1016/j.rvsc.2020.07.012 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in Veterinary Science 132 : 332-337 (October 2020) |
| spellingShingle | Ganado Bovino Rhipicephalus Plagas de Animales Control de Plagas Ivermectina Resistencia a los Acaricidas Argentina Cattle Pests of Animals Pest Control Ivermectin Resistance to Acaricides Garrapatas Rhipicephalus (Boophilus) microplus Torrents, Jorgelina Sarli, Macarena Rossner, Maria Victoria Toffaletti, Jose Rodolfo Morel, Nicolas Martinez, Norberto Claudio Webster, Anelise Mangold, Atilio Jose Guglielmone, Alberto Nava, Santiago Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title_full | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title_fullStr | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title_full_unstemmed | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title_short | Resistance of the cattle tick Rhipicephalus (Boophilus) microplus to ivermectin in Argentina |
| title_sort | resistance of the cattle tick rhipicephalus boophilus microplus to ivermectin in argentina |
| topic | Ganado Bovino Rhipicephalus Plagas de Animales Control de Plagas Ivermectina Resistencia a los Acaricidas Argentina Cattle Pests of Animals Pest Control Ivermectin Resistance to Acaricides Garrapatas Rhipicephalus (Boophilus) microplus |
| url | http://hdl.handle.net/20.500.12123/7736 https://www.sciencedirect.com/science/article/abs/pii/S0034528820309255 https://doi.org/10.1016/j.rvsc.2020.07.012 |
| work_keys_str_mv | AT torrentsjorgelina resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT sarlimacarena resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT rossnermariavictoria resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT toffalettijoserodolfo resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT morelnicolas resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT martineznorbertoclaudio resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT websteranelise resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT mangoldatiliojose resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT guglielmonealberto resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina AT navasantiago resistanceofthecattletickrhipicephalusboophilusmicroplustoivermectininargentina |