Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)

The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ and gut‐ extracts) of A. grandis were evaluated. Gut extract section from th...

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Autores principales: Ben Guerrero, Emiliano, Salvador, Ricardo, Talia, Paola Mónica
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/7723
https://onlinelibrary.wiley.com/doi/abs/10.1002/arch.21730
https://doi.org/10.1002/arch.21730
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author Ben Guerrero, Emiliano
Salvador, Ricardo
Talia, Paola Mónica
author_browse Ben Guerrero, Emiliano
Salvador, Ricardo
Talia, Paola Mónica
author_facet Ben Guerrero, Emiliano
Salvador, Ricardo
Talia, Paola Mónica
author_sort Ben Guerrero, Emiliano
collection INTA Digital
description The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ and gut‐ extracts) of A. grandis were evaluated. Gut extract section from third instar larvae exhibited endoglucanase, xylanase, β‐glucosidase, and pectinase activities. The endoglucanase and xylanase activities were localized in the foregut, whereas β‐glucosidase activity was mainly detected in the hindgut. In addition, no difference in pectinase activity was observed across the gut sections. Thus, A. grandis digestive system is a potentially interesting reservoir for further lignocellulolytic enzymes research.
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spelling INTA77232020-08-14T13:29:22Z Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae) Ben Guerrero, Emiliano Salvador, Ricardo Talia, Paola Mónica Anthonomus grandis Plagas de Plantas Algodón Enzimas Pests of Plants Cotton Enzymes The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ and gut‐ extracts) of A. grandis were evaluated. Gut extract section from third instar larvae exhibited endoglucanase, xylanase, β‐glucosidase, and pectinase activities. The endoglucanase and xylanase activities were localized in the foregut, whereas β‐glucosidase activity was mainly detected in the hindgut. In addition, no difference in pectinase activity was observed across the gut sections. Thus, A. grandis digestive system is a potentially interesting reservoir for further lignocellulolytic enzymes research. Instituto de Biotecnología Fil: Ben Guerrero, Emiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-08-14T13:21:52Z 2020-08-14T13:21:52Z 2020-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7723 https://onlinelibrary.wiley.com/doi/abs/10.1002/arch.21730 1520-6327 0739-4462 https://doi.org/10.1002/arch.21730 eng info:eu-repo/semantics/restrictedAccess application/pdf Wiley Archives of Insect Biochemistry and Physiology (First published: 01 August 2020)
spellingShingle Anthonomus grandis
Plagas de Plantas
Algodón
Enzimas
Pests of Plants
Cotton
Enzymes
Ben Guerrero, Emiliano
Salvador, Ricardo
Talia, Paola Mónica
Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title_full Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title_fullStr Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title_full_unstemmed Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title_short Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae)
title_sort evaluation of hydrolytic enzyme activities from digestive fluids of anthonomus grandis coleoptera curculionidae
topic Anthonomus grandis
Plagas de Plantas
Algodón
Enzimas
Pests of Plants
Cotton
Enzymes
url http://hdl.handle.net/20.500.12123/7723
https://onlinelibrary.wiley.com/doi/abs/10.1002/arch.21730
https://doi.org/10.1002/arch.21730
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