BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology
Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7706 https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624 https://doi.org/10.1016/j.rvsc.2020.03.001 |
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| author | Eirin, Maria Emilia Carignano, Hugo Adrian Shimizu, Ernesto Pando, Maria de los Angeles Zumarraga, Martin Jose Magnano, Gabriel Macías, Analía Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Morsella, Claudia Graciela Ferrara Muñiz, Ximena Cataldi, Angel Adrian Paolicchi, Fernando Poli, Mario Andres |
| author_browse | Carignano, Hugo Adrian Cataldi, Angel Adrian Eirin, Maria Emilia Ferrara Muñiz, Ximena Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Macías, Analía Magnano, Gabriel Morsella, Claudia Graciela Pando, Maria de los Angeles Paolicchi, Fernando Poli, Mario Andres Shimizu, Ernesto Zumarraga, Martin Jose |
| author_facet | Eirin, Maria Emilia Carignano, Hugo Adrian Shimizu, Ernesto Pando, Maria de los Angeles Zumarraga, Martin Jose Magnano, Gabriel Macías, Analía Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Morsella, Claudia Graciela Ferrara Muñiz, Ximena Cataldi, Angel Adrian Paolicchi, Fernando Poli, Mario Andres |
| author_sort | Eirin, Maria Emilia |
| collection | INTA Digital |
| description | Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7706 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA77062020-08-11T17:07:20Z BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology Eirin, Maria Emilia Carignano, Hugo Adrian Shimizu, Ernesto Pando, Maria de los Angeles Zumarraga, Martin Jose Magnano, Gabriel Macías, Analía Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Morsella, Claudia Graciela Ferrara Muñiz, Ximena Cataldi, Angel Adrian Paolicchi, Fernando Poli, Mario Andres Ganado Bovino Enfermedades de los Animales Polimorfismo Tuberculosis Mycobacterium bovis Cattle Animal Diseases Polymorphism Mycobacterium bovis Infections Antigens Infeccion por Mycobacterium bovis Antígenos Tuberculosis Bovina Bovine Leucocyte Antigen Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and disseminated worldwide. In Argentina, the highest prevalence occurs in dairy areas. BoLA DRB3.2 is related to the adaptive immunity in mycobacterial infections. Genetic polymorphisms of this marker have been associated with resistance or susceptibility to bovine diseases. We evaluated the association between BoLA DRB3.2 polymorphisms and bTB pathology scores in dairy and beef cattle breeds of Argentina. Most bovines exhibited visible lesions compatible with tuberculosis and, furthermore, 150 (85.7%) were also positive by bacteriology. A pathology index showed a variable degree of disease, from 3 to 76 (median pathology score = 9 (IQR: 7–15)). Thirty-five BoLA DRB3.2 alleles were identified with an associated frequency from 16% to 0.3%, distributed 73% (n = 128) in heterozygosis and 27% (n = 47) in homozygosis, with 12 BoLA DRB3.2 alleles (*0101, *1101, *1501, *0201, *2707 *1001, *1002, *1201, *14011, *0501 *0902 and *0701) representing the 74.7% of the population variability. A functional analysis grouped them in 4 out of 5 clusters (A-D), suggesting a functional overlapping. Among the 90 identified genotypes, *1101/*1101, *1101/*1501 and *0101/*0101 were the most frequent (10%, 8.9% and 8.9%, respectively). No association was detected between the pathology scores and a specific DRB3.2 allele (p > .05). Animals infected with M. bovis spoligotype SB0153 showed a significantly higher pathology score than those affected by the spoligotype SB0145 (p = .018). Furthermore, the Aberdeen Angus breed exhibited highest pathological scores (p < .0001), which were associated with disseminated lesion, thus suggesting that the host component could be important to the disease progression. Instituto de Biotecnología Fil: Eirin, Maria Emilia.Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Shimizu, Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pando, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Patología Animal; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Huertas, Pablo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata; Argentina Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2020-08-11T17:00:55Z 2020-08-11T17:00:55Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7706 https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624 0034-5288 https://doi.org/10.1016/j.rvsc.2020.03.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Research in Veterinary Science 130 : 118-125 (June 2020) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Polimorfismo Tuberculosis Mycobacterium bovis Cattle Animal Diseases Polymorphism Mycobacterium bovis Infections Antigens Infeccion por Mycobacterium bovis Antígenos Tuberculosis Bovina Bovine Leucocyte Antigen Eirin, Maria Emilia Carignano, Hugo Adrian Shimizu, Ernesto Pando, Maria de los Angeles Zumarraga, Martin Jose Magnano, Gabriel Macías, Analía Garbaccio, Sergio Gabriel Huertas, Pablo Sebastian Morsella, Claudia Graciela Ferrara Muñiz, Ximena Cataldi, Angel Adrian Paolicchi, Fernando Poli, Mario Andres BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title_full | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title_fullStr | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title_full_unstemmed | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title_short | BoLA-DRB3 exon2 polymorphisms among tuberculous cattle: Nucleotide and functional variability and their association with bovine tuberculosis pathology |
| title_sort | bola drb3 exon2 polymorphisms among tuberculous cattle nucleotide and functional variability and their association with bovine tuberculosis pathology |
| topic | Ganado Bovino Enfermedades de los Animales Polimorfismo Tuberculosis Mycobacterium bovis Cattle Animal Diseases Polymorphism Mycobacterium bovis Infections Antigens Infeccion por Mycobacterium bovis Antígenos Tuberculosis Bovina Bovine Leucocyte Antigen |
| url | http://hdl.handle.net/20.500.12123/7706 https://www.sciencedirect.com/science/article/abs/pii/S0034528819305624 https://doi.org/10.1016/j.rvsc.2020.03.001 |
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