Circadian oscillation of gibberellin signaling in Arabidopsis

Circadian clocks are endogenous timekeeping mechanisms that allow organisms to anticipate rhythmic, daily environmental changes. Temporal coordination of transcription results in a set of gene expression patterns with peak levels occurring at precise times of the day. An intriguing question is how...

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Autores principales: Arana, María Veronica, Marín de la Rosa, Nora, Maloof, Julin N., Blazquez, Miguel Angel, Alabadí, David
Formato: Artículo
Lenguaje:Inglés
Publicado: National Academy of Sciences 2020
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/7683
https://www.pnas.org/content/108/22/9292
https://doi.org/10.1073/pnas.1101050108
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author Arana, María Veronica
Marín de la Rosa, Nora
Maloof, Julin N.
Blazquez, Miguel Angel
Alabadí, David
author_browse Alabadí, David
Arana, María Veronica
Blazquez, Miguel Angel
Maloof, Julin N.
Marín de la Rosa, Nora
author_facet Arana, María Veronica
Marín de la Rosa, Nora
Maloof, Julin N.
Blazquez, Miguel Angel
Alabadí, David
author_sort Arana, María Veronica
collection INTA Digital
description Circadian clocks are endogenous timekeeping mechanisms that allow organisms to anticipate rhythmic, daily environmental changes. Temporal coordination of transcription results in a set of gene expression patterns with peak levels occurring at precise times of the day. An intriguing question is how a single clock can generate different oscillatory rhythms, and it has been proposed that hormone signaling might act in plants as a relay mechanism to modulate the amplitude and the phase of output rhythms. Here we show that the circadian clock gates gibberellin (GA) signaling through transcriptional regulation of the GA receptors, resulting in higher stability of DELLA proteins during daytime and higher GA sensitivity at night. Oscillation of GA signaling appears to be particularly critical for rhythmic growth, given that constitutive expression of the GA receptor expands the daily growth period in seedlings, and complete loss of DELLA function causes continuous, arrhythmic hypocotyl growth. Moreover, transcriptomic analysis of a pentuple della KO mutant indicates that the GA pathway mediates the rhythmic expression of many clock-regulated genes related to biotic and abiotic stress responses and cell wall modification. Thus, gating of GA sensitivity by the circadian clock represents an additional layer of regulation that might provide extra robustness to the diurnal growth rhythm and constitute a regulatory module that coordinates the circadian clock with additional endogenous and environmental signals.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA76832020-08-06T19:30:52Z Circadian oscillation of gibberellin signaling in Arabidopsis Arana, María Veronica Marín de la Rosa, Nora Maloof, Julin N. Blazquez, Miguel Angel Alabadí, David Plasticidad Fenotípica Fotoperiodismo Arabidopsis Phenotypic Plasticity Photoperiodicity Reloj Circadiano Circadian clocks are endogenous timekeeping mechanisms that allow organisms to anticipate rhythmic, daily environmental changes. Temporal coordination of transcription results in a set of gene expression patterns with peak levels occurring at precise times of the day. An intriguing question is how a single clock can generate different oscillatory rhythms, and it has been proposed that hormone signaling might act in plants as a relay mechanism to modulate the amplitude and the phase of output rhythms. Here we show that the circadian clock gates gibberellin (GA) signaling through transcriptional regulation of the GA receptors, resulting in higher stability of DELLA proteins during daytime and higher GA sensitivity at night. Oscillation of GA signaling appears to be particularly critical for rhythmic growth, given that constitutive expression of the GA receptor expands the daily growth period in seedlings, and complete loss of DELLA function causes continuous, arrhythmic hypocotyl growth. Moreover, transcriptomic analysis of a pentuple della KO mutant indicates that the GA pathway mediates the rhythmic expression of many clock-regulated genes related to biotic and abiotic stress responses and cell wall modification. Thus, gating of GA sensitivity by the circadian clock represents an additional layer of regulation that might provide extra robustness to the diurnal growth rhythm and constitute a regulatory module that coordinates the circadian clock with additional endogenous and environmental signals. Fil: Arana, María Veronica. Intituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marín de la Rosa, Nora. Universitat Politecnica de Valencia. Instituto de Biología Molecular y Celular de Plantas; España Fil: Maloof, Julin N. University of California. Department of Plant Biology; Estados Unidos Fil: Blazquez, Miguel Angel. Universidad Politécnica de Valencia. Consejo Superior de Investigaciones Científicas. Institute of Molecular and Plant Cellular Biology; España Fil: Alabadí, David. Universitat Politecnica de Valencia. Instituto de Biología Molecular y Celular de Plantas; España 2020-08-06T19:21:42Z 2020-08-06T19:21:42Z 2011-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7683 https://www.pnas.org/content/108/22/9292 1091-6490 0027-8424 https://doi.org/10.1073/pnas.1101050108 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf National Academy of Sciences PNAS 108 (22) : 9292-9297 (Mayo 2011)
spellingShingle Plasticidad Fenotípica
Fotoperiodismo
Arabidopsis
Phenotypic Plasticity
Photoperiodicity
Reloj Circadiano
Arana, María Veronica
Marín de la Rosa, Nora
Maloof, Julin N.
Blazquez, Miguel Angel
Alabadí, David
Circadian oscillation of gibberellin signaling in Arabidopsis
title Circadian oscillation of gibberellin signaling in Arabidopsis
title_full Circadian oscillation of gibberellin signaling in Arabidopsis
title_fullStr Circadian oscillation of gibberellin signaling in Arabidopsis
title_full_unstemmed Circadian oscillation of gibberellin signaling in Arabidopsis
title_short Circadian oscillation of gibberellin signaling in Arabidopsis
title_sort circadian oscillation of gibberellin signaling in arabidopsis
topic Plasticidad Fenotípica
Fotoperiodismo
Arabidopsis
Phenotypic Plasticity
Photoperiodicity
Reloj Circadiano
url http://hdl.handle.net/20.500.12123/7683
https://www.pnas.org/content/108/22/9292
https://doi.org/10.1073/pnas.1101050108
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