Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex
Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several spec...
| Main Authors: | , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7682 https://www.sciencedirect.com/science/article/pii/S0304401719302985 https://doi.org/10.1016/j.vetpar.2019.109017 |
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| author | Avila, Héctor Gabriel Mozzoni, Cecilia Trangoni, Marcos David Cravero, Silvio Lorenzo Pedro Pérez, Verónica Mirtha Valenzuela, Federico Gertiser, María Laura Butti, Marcos Javier Kamenetzky, Laura Jensen, Oscar Rosenzvit, Mara Cecilia |
| author_browse | Avila, Héctor Gabriel Butti, Marcos Javier Cravero, Silvio Lorenzo Pedro Gertiser, María Laura Jensen, Oscar Kamenetzky, Laura Mozzoni, Cecilia Pérez, Verónica Mirtha Rosenzvit, Mara Cecilia Trangoni, Marcos David Valenzuela, Federico |
| author_facet | Avila, Héctor Gabriel Mozzoni, Cecilia Trangoni, Marcos David Cravero, Silvio Lorenzo Pedro Pérez, Verónica Mirtha Valenzuela, Federico Gertiser, María Laura Butti, Marcos Javier Kamenetzky, Laura Jensen, Oscar Rosenzvit, Mara Cecilia |
| author_sort | Avila, Héctor Gabriel |
| collection | INTA Digital |
| description | Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7682 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA76822020-08-06T17:22:31Z Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex Avila, Héctor Gabriel Mozzoni, Cecilia Trangoni, Marcos David Cravero, Silvio Lorenzo Pedro Pérez, Verónica Mirtha Valenzuela, Federico Gertiser, María Laura Butti, Marcos Javier Kamenetzky, Laura Jensen, Oscar Rosenzvit, Mara Cecilia Echinococcus granulosus Equinococosis Epidemiología Zoonosis Diagnóstico Echinococcosis Epidemiology Zoonoses Diagnosis Cystic echinococcosis represents a significant problem in human and animal health and constitutes one of the most severe Neglected Tropical Diseases prioritized by the World Health Organization. The etiological agent is the complex Echinococcus granulosus sensu lato (s. l.), composed of several species/genotypes. Diagnosis in the definitive host and molecular epidemiology studies are important points for cystic echinococcosis control. Here we developed a new copro-LAMP assay, LAMP EGSL, for diagnosis in the definitive host for simultaneous detection of Echinococcus granulosus sensu stricto (s. s.), Echinococcus ortleppi, and Echinococcus canadensis species. Also, the analytical sensitivity, specificity and plausibility of performance in a rural context of a previously reported species-specific LAMP reaction, was evaluated. Both reactions showed high analytical sensitivity values (10 fg-100 fg DNA) and did not show cross reaction with DNA from host or other helminthic parasites. LAMP EGSL was performed with samples from an endemic area. In addition, the alkaline hydrolysis of one E. granulosus s. s. adult parasite followed by specific LAMP to E. granulosus s. s. was performed in a laboratory with low resources from another cystic echinococcosis endemic area. The results obtained suggest that LAMP EGSL represents a potential tool for canine diagnosis that could be useful for cystic echinococcosis control programs. In addition, we showed that LAMP reaction for E. granulous s. s., E. ortleppi and E. canadensis specific detection, could be useful for molecular epidemiology studies applicable to the definitive host. Both reactions were performed in endemic, rural areas without sophisticated equipment. Instituto de Biotecnología Fil: Avila, Héctor Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Centro de Investigación en Zoonosis (Chubut); Argentina Fil: Mozzoni, Cecilia. Santa Cruz (Argentina : provincia). Ministerio de Salud y Ambiente. Hospital Zonal Caleta Olivia; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Brucella, Campylobacter & Microbiota; Argentina. Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Laboratorio de Brucella, Campylobacter & Microbiota; Argentina Fil: Pérez, Verónica Mirtha. San Juan (Argentina : provincia). División Zoonosis; Argentina Fil: Valenzuela, Federico. San Juan (Argentina : provincia). División Zoonosis; Argentina Fil: Gertiser, María Laura. Centro de Investigación en Zoonosis (Chubut); Argentina Fil: Butti, Marcos Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Parasitología Comparada. Laboratorio de Parasitosis Humanas y Zoonosis Parasitarias; Argentina Fil: Kamenetzky, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Fil: Jensen, Oscar. Centro de Investigación en Zoonosis (Chubut); Argentina Fil: Rosenzvit, Mara Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. 2020-08-06T16:47:31Z 2020-08-06T16:47:31Z 2020-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7682 https://www.sciencedirect.com/science/article/pii/S0304401719302985 0304-4017 https://doi.org/10.1016/j.vetpar.2019.109017 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Parasitology 277 : 109017 (January 2020) |
| spellingShingle | Echinococcus granulosus Equinococosis Epidemiología Zoonosis Diagnóstico Echinococcosis Epidemiology Zoonoses Diagnosis Avila, Héctor Gabriel Mozzoni, Cecilia Trangoni, Marcos David Cravero, Silvio Lorenzo Pedro Pérez, Verónica Mirtha Valenzuela, Federico Gertiser, María Laura Butti, Marcos Javier Kamenetzky, Laura Jensen, Oscar Rosenzvit, Mara Cecilia Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title | Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title_full | Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title_fullStr | Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title_full_unstemmed | Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title_short | Development of a copro-LAMP assay for detection of several species of Echinococcus granulosus sensu lato complex |
| title_sort | development of a copro lamp assay for detection of several species of echinococcus granulosus sensu lato complex |
| topic | Echinococcus granulosus Equinococosis Epidemiología Zoonosis Diagnóstico Echinococcosis Epidemiology Zoonoses Diagnosis |
| url | http://hdl.handle.net/20.500.12123/7682 https://www.sciencedirect.com/science/article/pii/S0304401719302985 https://doi.org/10.1016/j.vetpar.2019.109017 |
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