A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms
Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strate...
| Autores principales: | , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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BMC
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7673 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-3 https://doi.org/10.1186/s12917-020-02476-3 |
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| author | Vega, Celina Guadalupe Bok, Marina Ebinger, Maren Rocha, Lucía Alejandra Rivolta, Alejandra Antonella González Thomas, Valeria Muntadas, Pilar D’Aloia, Ricardo Pinto, Verónica Parreño, Viviana Wigdorovitz, Andres |
| author_browse | Bok, Marina D’Aloia, Ricardo Ebinger, Maren González Thomas, Valeria Muntadas, Pilar Parreño, Viviana Pinto, Verónica Rivolta, Alejandra Antonella Rocha, Lucía Alejandra Vega, Celina Guadalupe Wigdorovitz, Andres |
| author_facet | Vega, Celina Guadalupe Bok, Marina Ebinger, Maren Rocha, Lucía Alejandra Rivolta, Alejandra Antonella González Thomas, Valeria Muntadas, Pilar D’Aloia, Ricardo Pinto, Verónica Parreño, Viviana Wigdorovitz, Andres |
| author_sort | Vega, Celina Guadalupe |
| collection | INTA Digital |
| description | Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time.
Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves.
Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7673 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA76732022-08-12T13:55:43Z A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms Vega, Celina Guadalupe Bok, Marina Ebinger, Maren Rocha, Lucía Alejandra Rivolta, Alejandra Antonella González Thomas, Valeria Muntadas, Pilar D’Aloia, Ricardo Pinto, Verónica Parreño, Viviana Wigdorovitz, Andres Ganado de Leche Enfermedades de los Animales Respuesta Inmunológica Anticuerpos Rota Virus Diarrea de Terneros Dairy Cattle Animal Diseases Immune Response Antibodies Calf Diarrhoea Rotavirus Diarrea Neonatal Background: Neonatal diarrhea remains one of the main causes of morbi-mortality in dairy calves under artificial rearing. It is often caused by infectious agents of viral, bacterial, or parasitic origin. Cows vaccination and colostrum intake by calves during the first 6 h of life are critical strategies to prevent severe diarrhea but these are still insufficient. Here we report the field evaluation of a product based on IgY antibodies against group A rotavirus (RVA), coronavirus (CoV), enterotoxigenic Escherichia coli, and Salmonella sp. This product, named IgY DNT, has been designed as a complementary passive immunization strategy to prevent neonatal calf diarrhea. The quality of the product depends on the titers of specific IgY antibodies to each antigen evaluated by ELISA. In the case of the viral antigens, ELISA antibody (Ab) titers are correlated with protection against infection in calves experimentally challenged with RVA and CoV (Bok M, et al., Passive immunity to control bovine coronavirus diarrhea in a dairy herd in Argentina, 2017), (Vega C, et al., Vet Immunol Immunopathol, 142:156–69, 2011), (Vega C, et al., Res Vet Sci, 103:1–10, 2015). To evaluate the efficiency in dairy farms, thirty newborn Holstein calves were randomly assigned to IgY DNT or control groups and treatment initiated after colostrum intake and gut closure. Calves in the IgY DNT group received 20 g of the oral passive treatment in 2 L of milk twice a day during the first 2 weeks of life. Animals were followed until 3 weeks of age and diarrhea due to natural exposure to infectious agents was recorded during all the experimental time. Results: Results demonstrate that the oral administration of IgY DNT during the first 2 weeks of life to newborn calves caused a delay in diarrhea onset and significantly reduced its severity and duration compared with untreated calves. Animals treated with IgY DNT showed a trend towards a delay in RVA infection with significantly shorter duration and virus shedding compared to control calves. Conclusions: This indicates that IgY DNT is an effective product to complement current preventive strategies against neonatal calf diarrhea in dairy farms. Furthermore, to our knowledge, this is the only biological product available for the prevention of virus-associated neonatal calf diarrhea. Centro de Investigación en Ciencias Veterinarias y Agronómicas Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina Fil: Ebinger, Maren. El Mangrullo Farm; Argentina Fil: Rocha, Lucía Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: González Thomas, Valeria. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Muntadas, Pilar. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: D’Aloia, Ricardo. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Pinto, Verónica. Vetanco S.A.; Argentina Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Incuinta e Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Bioinnovo S.A.; Argentina 2020-08-05T16:54:58Z 2020-08-05T16:54:58Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7673 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-3 1746-6148 https://doi.org/10.1186/s12917-020-02476-3 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC BMC Veterinary Research 16 : article number: 264 (2020) |
| spellingShingle | Ganado de Leche Enfermedades de los Animales Respuesta Inmunológica Anticuerpos Rota Virus Diarrea de Terneros Dairy Cattle Animal Diseases Immune Response Antibodies Calf Diarrhoea Rotavirus Diarrea Neonatal Vega, Celina Guadalupe Bok, Marina Ebinger, Maren Rocha, Lucía Alejandra Rivolta, Alejandra Antonella González Thomas, Valeria Muntadas, Pilar D’Aloia, Ricardo Pinto, Verónica Parreño, Viviana Wigdorovitz, Andres A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title | A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title_full | A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title_fullStr | A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title_full_unstemmed | A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title_short | A new passive immune strategy based on IgY antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| title_sort | new passive immune strategy based on igy antibodies as a key element to control neonatal calf diarrhea in dairy farms |
| topic | Ganado de Leche Enfermedades de los Animales Respuesta Inmunológica Anticuerpos Rota Virus Diarrea de Terneros Dairy Cattle Animal Diseases Immune Response Antibodies Calf Diarrhoea Rotavirus Diarrea Neonatal |
| url | http://hdl.handle.net/20.500.12123/7673 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-020-02476-3 https://doi.org/10.1186/s12917-020-02476-3 |
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