Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7664 https://www.sciencedirect.com/science/article/abs/pii/S0147957120301119 https://doi.org/10.1016/j.cimid.2020.101522 |
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| author | Fiorani, Franco Hecker, Yanina Cirone, Karina Mariela Armendano, Joaquín Ignacio Gual, Ignacio Campero, Lucía María Wilkowsky, Silvina Elizabeth Cobo, Eduardo Rubén Corva, Pablo Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin |
| author_browse | Armendano, Joaquín Ignacio Campero, Lucía María Canton, German Jose Cirone, Karina Mariela Cobo, Eduardo Rubén Corva, Pablo Fiorani, Franco Gual, Ignacio Hecker, Yanina Moore, Prando Dadin Morrell, Eleonora Lidia Wilkowsky, Silvina Elizabeth |
| author_facet | Fiorani, Franco Hecker, Yanina Cirone, Karina Mariela Armendano, Joaquín Ignacio Gual, Ignacio Campero, Lucía María Wilkowsky, Silvina Elizabeth Cobo, Eduardo Rubén Corva, Pablo Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin |
| author_sort | Fiorani, Franco |
| collection | INTA Digital |
| description | Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral responders |
| format | Artículo |
| id | INTA7664 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA76642020-08-04T11:29:30Z Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies Fiorani, Franco Hecker, Yanina Cirone, Karina Mariela Armendano, Joaquín Ignacio Gual, Ignacio Campero, Lucía María Wilkowsky, Silvina Elizabeth Cobo, Eduardo Rubén Corva, Pablo Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Vaca Neospora Caninun Prueba Cutáneas Hipersensibilidad Retardada Respuesta Inmunológica Cows Skin Tests Delayed Hypersensitivity Immune Response Heifers Novilla Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral responders EEA Balcarce Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil. Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina Fil.: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA) Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA.) Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina. Fil: Wilkowsky, Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine; Canada Fil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina Fil: Morrell, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina 2020-08-04T11:17:06Z 2020-08-04T11:17:06Z 2020-07-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7664 https://www.sciencedirect.com/science/article/abs/pii/S0147957120301119 0147-9571 https://doi.org/10.1016/j.cimid.2020.101522 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 72 : 101522 (2020) |
| spellingShingle | Vaca Neospora Caninun Prueba Cutáneas Hipersensibilidad Retardada Respuesta Inmunológica Cows Skin Tests Delayed Hypersensitivity Immune Response Heifers Novilla Fiorani, Franco Hecker, Yanina Cirone, Karina Mariela Armendano, Joaquín Ignacio Gual, Ignacio Campero, Lucía María Wilkowsky, Silvina Elizabeth Cobo, Eduardo Rubén Corva, Pablo Morrell, Eleonora Lidia Canton, German Jose Moore, Prando Dadin Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title | Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title_full | Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title_fullStr | Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title_full_unstemmed | Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title_short | Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies |
| title_sort | delayed type hypersensitivity skin test against neospora caninum in heifers with undetectable specific antibodies |
| topic | Vaca Neospora Caninun Prueba Cutáneas Hipersensibilidad Retardada Respuesta Inmunológica Cows Skin Tests Delayed Hypersensitivity Immune Response Heifers Novilla |
| url | http://hdl.handle.net/20.500.12123/7664 https://www.sciencedirect.com/science/article/abs/pii/S0147957120301119 https://doi.org/10.1016/j.cimid.2020.101522 |
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