Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina
Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalen...
| Autores principales: | , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7633 https://link.springer.com/article/10.1007/s11262-020-01783-z https://doi.org/10.1007/s11262-020-01783-z |
| _version_ | 1855484046941880320 |
|---|---|
| author | Trucco, Veronica Milagros Cabrera Mederos, Dariel Lenardon, Sergio Luis Giolitti, Fabian |
| author_browse | Cabrera Mederos, Dariel Giolitti, Fabian Lenardon, Sergio Luis Trucco, Veronica Milagros |
| author_facet | Trucco, Veronica Milagros Cabrera Mederos, Dariel Lenardon, Sergio Luis Giolitti, Fabian |
| author_sort | Trucco, Veronica Milagros |
| collection | INTA Digital |
| description | Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide. |
| format | Artículo |
| id | INTA7633 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA76332020-07-29T12:39:43Z Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina Trucco, Veronica Milagros Cabrera Mederos, Dariel Lenardon, Sergio Luis Giolitti, Fabian Medicago sativa Virus de las Plantas Enamovirus Distribución Geográfica Genética Vectores Argentina Plant Viruses Geographical Distribution Genetics Vectors Alfalfa Lucerne Alfalfa (Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide. Instituto de Patología Vegetal Fil: Trucco, Verónica Milagros. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina Fil: Lenardon, Sergio Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina Fil: Giolitti, Fabián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal. Unidad de Fitopatología y Modernización Agrícola -UFyMA- CONICET;Argentina 2020-07-29T12:20:58Z 2020-07-29T12:20:58Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7633 https://link.springer.com/article/10.1007/s11262-020-01783-z 0920-8569 1572-994X https://doi.org/10.1007/s11262-020-01783-z eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Virus Genes (2020) |
| spellingShingle | Medicago sativa Virus de las Plantas Enamovirus Distribución Geográfica Genética Vectores Argentina Plant Viruses Geographical Distribution Genetics Vectors Alfalfa Lucerne Trucco, Veronica Milagros Cabrera Mederos, Dariel Lenardon, Sergio Luis Giolitti, Fabian Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title | Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title_full | Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title_fullStr | Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title_full_unstemmed | Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title_short | Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina |
| title_sort | geographical distribution genetic studies and vector transmission of alfalfa enamovirus 1 infecting alfalfa crop in argentina |
| topic | Medicago sativa Virus de las Plantas Enamovirus Distribución Geográfica Genética Vectores Argentina Plant Viruses Geographical Distribution Genetics Vectors Alfalfa Lucerne |
| url | http://hdl.handle.net/20.500.12123/7633 https://link.springer.com/article/10.1007/s11262-020-01783-z https://doi.org/10.1007/s11262-020-01783-z |
| work_keys_str_mv | AT truccoveronicamilagros geographicaldistributiongeneticstudiesandvectortransmissionofalfalfaenamovirus1infectingalfalfacropinargentina AT cabreramederosdariel geographicaldistributiongeneticstudiesandvectortransmissionofalfalfaenamovirus1infectingalfalfacropinargentina AT lenardonsergioluis geographicaldistributiongeneticstudiesandvectortransmissionofalfalfaenamovirus1infectingalfalfacropinargentina AT giolittifabian geographicaldistributiongeneticstudiesandvectortransmissionofalfalfaenamovirus1infectingalfalfacropinargentina |