Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice
In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotypi...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7629 https://link.springer.com/article/10.1007/s10681-020-02661-3 https://doi.org/10.1007/s10681-020-02661-3 |
| _version_ | 1855035894074966016 |
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| author | Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_browse | Arguissain, Gustavo Gabriel Bonell, Martha Lucrecia Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_facet | Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia |
| author_sort | Bonell, Martha Lucrecia |
| collection | INTA Digital |
| description | In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7629 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA76292020-07-28T17:23:01Z Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos In Argentina, the yield potential of rice (Oryza sativa L.) under suboptimal temperatures of temperate regions and during early sowing in tropical regions depends on its low-temperature germinability (LTG) and subsequent cold tolerance at the seedling stage (CTS). This study focused on the phenotyping and identification of contrasting henotypes for LTG and CTS of 18 rice genotypes genotyped with 49 markers previously linked to cold tolerance. The selected markers revealed phenotypic variability among the genotypes evaluated. The results also demonstrated that both traits are regulated by different genes and that CTS is associated with differential alleles of candidate gene (CG)-based markers. Biplot clustering analysis allowed the selection of two cultivars, H298a/90 and Puitá INTA CL, contrasting for LTG, CTS and CG alleles. An F2 population (n = 128) derived from these two cultivars was genotyped with 43 SSR- and CG-based markers and 89 F2:3 families (~ 1800 seedlings) phenotyped for CTS. Single-marker analysis detected two major QTL that had been previously reported for CTS: qCTS11, which explained 18.35% of the phenotypic variance at 7 days of treatment, and qCTS12, which explained 26.62% and 47.94% of the phenotypic variance at 7 and 14 days of treatment, respectively. Marker-assisted introgression of QTL alleles favorable for CTS into Puitá INTA CL, which has high LTG, could contribute to obtaining breeding lines with early seedling vigor adapted to the sowing season in Argentina. EEA Concepción del Uruguay Fil: Bonell, Martha Lucrecia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Arguissain, Gustavo Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Crepy, Maria Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Fernandez, Paula Del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heinz, Ruth Amelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-07-28T17:15:57Z 2020-07-28T17:15:57Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7629 https://link.springer.com/article/10.1007/s10681-020-02661-3 0014-2336 1573-5060 https://doi.org/10.1007/s10681-020-02661-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Euphytica 216 : article number: 128 (2020) |
| spellingShingle | Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos Bonell, Martha Lucrecia Arguissain, Gustavo Gabriel Crepy, Maria Andrea Fernandez, Paula Del Carmen Heinz, Ruth Amelia Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title | Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_full | Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_fullStr | Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_full_unstemmed | Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_short | Phenotyping and QTL mapping for cold tolerance at the germination and seedling stages in Argentine temperate rice |
| title_sort | phenotyping and qtl mapping for cold tolerance at the germination and seedling stages in argentine temperate rice |
| topic | Arroz Tolerancia al Frío Fenotipos Loci de Rasgos Cuantitativos Argentina Rice Cold Tolerance Phenotypes Quantitative Trait Loci QTL (quantitative trait loci) Genes Candidatos |
| url | http://hdl.handle.net/20.500.12123/7629 https://link.springer.com/article/10.1007/s10681-020-02661-3 https://doi.org/10.1007/s10681-020-02661-3 |
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