A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp.
Introduction: Babesiosis is a protozoan tick-borne infection associated with anemia and life-threatening disease in humans, domestic and wildlife animals. Dogs are infected by at least six well-characterized Babesia spp. that cause clinical disease. Infection with a piroplasmid species was detected...
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| Format: | Artículo |
| Language: | Inglés |
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BMC
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7584 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-3995-5 https://doi.org/10.1186/s13071-020-3995-5 |
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| author | Baneth, Gad Nachum‑Biala, Yaarit Birkenheuer, Adam Joseph Schreeg, Megan Elizabeth Prince, Hagar Florin-Christensen, Monica Schnittger, Leonhard Aroch, Itamar |
| author_browse | Aroch, Itamar Baneth, Gad Birkenheuer, Adam Joseph Florin-Christensen, Monica Nachum‑Biala, Yaarit Prince, Hagar Schnittger, Leonhard Schreeg, Megan Elizabeth |
| author_facet | Baneth, Gad Nachum‑Biala, Yaarit Birkenheuer, Adam Joseph Schreeg, Megan Elizabeth Prince, Hagar Florin-Christensen, Monica Schnittger, Leonhard Aroch, Itamar |
| author_sort | Baneth, Gad |
| collection | INTA Digital |
| description | Introduction: Babesiosis is a protozoan tick-borne infection associated with anemia and life-threatening disease in humans, domestic and wildlife animals. Dogs are infected by at least six well-characterized Babesia spp. that cause clinical disease. Infection with a piroplasmid species was detected by light microscopy of stained blood smears from five sick dogs from Israel and prompted an investigation on the parasite’s identity.
Methods: Genetic characterization of the piroplasmid was performed by PCR amplification of the 18S rRNA and the cytochrome c oxidase subunit 1 (cox1) genes, DNA sequencing and phylogenetic analysis. Four of the dogs were co-infected with Borrelia persica (Dschunkowsky, 1913), a relapsing fever spirochete transmitted by the argasid tick Ornithodoros tholozani Laboulbène & Mégnin. Co-infection of dogs with B. persica raised the possibility of transmission by O. tholozani and therefore, a piroplasmid PCR survey of ticks from this species was performed.
Results: The infected dogs presented with fever (4/5), anemia, thrombocytopenia (4/5) and icterus (3/5). Comparison of the 18S rRNA and cox1 piroplasmid gene sequences revealed 99–100% identity between sequences amplified from different dogs and ticks. Phylogenetic trees demonstrated a previously undescribed species of Babesia belonging to the western group of Babesia (sensu lato) and closely related to the human pathogen Babesia duncani Conrad, Kjemtrup, Carreno, Thomford, Wainwright, Eberhard, Quick, Telfrom & Herwalt, 2006 while more moderately related to Babesia conradae Kjemtrup, Wainwright, Miller, Penzhorn & Carreno, 2006 which infects dogs. The piroplasm forms detected included tetrads (Maltese cross), merozoite and trophozoite stages whose average size was larger than stages of other canine Babesia spp. belonging to the Babesia (s.l.) and B. gibsoni Patton, 1910, and smaller than other canine Babesia (sensu stricto) spp. Of 212 O. tholozani ticks surveyed, 11 (5.2%) harbored DNA of the new species of Babesia.
Conclusions: Babesia negevi n. sp. is described based on morphological and genetic characterization and phylogenetic analyses. The species is named after the Negev desert of southern Israel, where the first infected dog originated from. Despite co-infection in four dogs, the fifth dog had fatal disease attesting that B. negevi n. sp. infection requires clinical attention. Incriminating O. tholozani or another tick species as the vector of Babesia negevi n. sp., would require additional studies. |
| format | Artículo |
| id | INTA7584 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | INTA75842020-07-21T14:53:30Z A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. Baneth, Gad Nachum‑Biala, Yaarit Birkenheuer, Adam Joseph Schreeg, Megan Elizabeth Prince, Hagar Florin-Christensen, Monica Schnittger, Leonhard Aroch, Itamar Babesia Babesiosis Enfermedades de los Animales Perro Identificación Animal Diseases Dogs Identification Babesia negevi Piroplasmas Introduction: Babesiosis is a protozoan tick-borne infection associated with anemia and life-threatening disease in humans, domestic and wildlife animals. Dogs are infected by at least six well-characterized Babesia spp. that cause clinical disease. Infection with a piroplasmid species was detected by light microscopy of stained blood smears from five sick dogs from Israel and prompted an investigation on the parasite’s identity. Methods: Genetic characterization of the piroplasmid was performed by PCR amplification of the 18S rRNA and the cytochrome c oxidase subunit 1 (cox1) genes, DNA sequencing and phylogenetic analysis. Four of the dogs were co-infected with Borrelia persica (Dschunkowsky, 1913), a relapsing fever spirochete transmitted by the argasid tick Ornithodoros tholozani Laboulbène & Mégnin. Co-infection of dogs with B. persica raised the possibility of transmission by O. tholozani and therefore, a piroplasmid PCR survey of ticks from this species was performed. Results: The infected dogs presented with fever (4/5), anemia, thrombocytopenia (4/5) and icterus (3/5). Comparison of the 18S rRNA and cox1 piroplasmid gene sequences revealed 99–100% identity between sequences amplified from different dogs and ticks. Phylogenetic trees demonstrated a previously undescribed species of Babesia belonging to the western group of Babesia (sensu lato) and closely related to the human pathogen Babesia duncani Conrad, Kjemtrup, Carreno, Thomford, Wainwright, Eberhard, Quick, Telfrom & Herwalt, 2006 while more moderately related to Babesia conradae Kjemtrup, Wainwright, Miller, Penzhorn & Carreno, 2006 which infects dogs. The piroplasm forms detected included tetrads (Maltese cross), merozoite and trophozoite stages whose average size was larger than stages of other canine Babesia spp. belonging to the Babesia (s.l.) and B. gibsoni Patton, 1910, and smaller than other canine Babesia (sensu stricto) spp. Of 212 O. tholozani ticks surveyed, 11 (5.2%) harbored DNA of the new species of Babesia. Conclusions: Babesia negevi n. sp. is described based on morphological and genetic characterization and phylogenetic analyses. The species is named after the Negev desert of southern Israel, where the first infected dog originated from. Despite co-infection in four dogs, the fifth dog had fatal disease attesting that B. negevi n. sp. infection requires clinical attention. Incriminating O. tholozani or another tick species as the vector of Babesia negevi n. sp., would require additional studies. Instituto de Patobiología Fil: Baneth, Gad. Hebrew University. Koret School of Veterinary Medicine; Israel Fil: Nachum‑Biala, Yaarit. Hebrew University. Koret School of Veterinary Medicine; Israel Fil: Birkenheuer, Adam Joseph. North Carolina State University; Estados Unidos Fil: Schreeg, Megan Elizabeth. North Carolina State University; Estados Unidos Fil: Prince, Hagar. Hebrew University. Koret School of Veterinary Medicine; Israel Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Aroch, Itamar. Hebrew University. Koret School of Veterinary Medicine; Israel 2020-07-21T14:47:00Z 2020-07-21T14:47:00Z 2020-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7584 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-3995-5 1756-3305 https://doi.org/10.1186/s13071-020-3995-5 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BMC Parasites & Vectors 13 : article number: 130 (2020) |
| spellingShingle | Babesia Babesiosis Enfermedades de los Animales Perro Identificación Animal Diseases Dogs Identification Babesia negevi Piroplasmas Baneth, Gad Nachum‑Biala, Yaarit Birkenheuer, Adam Joseph Schreeg, Megan Elizabeth Prince, Hagar Florin-Christensen, Monica Schnittger, Leonhard Aroch, Itamar A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title | A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title_full | A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title_fullStr | A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title_full_unstemmed | A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title_short | A new piroplasmid species infecting dogs: morphological and molecular characterization and pathogeny of Babesia negevi n. sp. |
| title_sort | new piroplasmid species infecting dogs morphological and molecular characterization and pathogeny of babesia negevi n sp |
| topic | Babesia Babesiosis Enfermedades de los Animales Perro Identificación Animal Diseases Dogs Identification Babesia negevi Piroplasmas |
| url | http://hdl.handle.net/20.500.12123/7584 https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-3995-5 https://doi.org/10.1186/s13071-020-3995-5 |
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